Detection of choline in biological fluids from patients on haemodialysis by an amperometric biosensor based on a novel anti-interference bilayer

Guerrieri, Antonio; Ciriello, Rosanna; Crispo, Fabiana; Bianco, Giuliana

doi:10.1016/j.bioelechem.2019.05.009

Cited by 16 publications

(18 citation statements)

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“…Furthermore, the presence of the underlying overoxidized polypyrrole film guaranteed a notable rejection of interferent compounds, electroactive at the high overpotential required to detect H 2 O 2 , thus allowing the application of the sensor to assay Cholinesterase in real samples as complex as human serum [29]. Electrochemical, XPS, and ESEM characterizations of these biosensors were already described elsewhere [28][29][30][32][33][34][35]. Based on these important achievements, in the present work we wanted to employ such a biosensor to develop a novel PLD assay method.…”

Section: Scheme 1 Fabrication Steps Of the Biosensor (A) And Currentmentioning

confidence: 99%

Assay of Phospholipase D Activity by an Amperometric Choline Oxidase Biosensor

Ciriello

Guerrieri

2020

Sensors

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A novel electrochemical method to assay phospholipase D (PLD) activity is proposed based on the employment of a choline biosensor realized by immobilizing choline oxidase through co-crosslinking on an overoxidized polypyrrole film previously deposited on a platinum electrode. To perform the assay, an aliquot of a PLD standard solution is typically added to borate buffer containing phosphatidylcholine at a certain concentration and the oxidation current of hydrogen peroxide is then measured at the rotating modified electrode by applying a detection potential of +0.7 V vs. SCE. Various experimental parameters influencing the assay were studied and optimized. The employment of 0.75% (v/v) Triton X-100, 0.2 mM calcium chloride, 5 mM phosphatidylcholine, and borate buffer at pH 8.0, ionic strength (I) 0.05 M allowed to achieve considerable current responses. In order to assure a controlled mass transport and, at the same time, high sensitivity, an electrode rotation rate of 200 rpm was selected. The proposed method showed a sensitivity of 24 (nA/s)⋅(IU/mL)−1, a wide linear range up to 0.33 IU/mL, fast response time and appreciable long-term stability. The limit of detection, evaluated from the linear calibration curve, was 0.005 IU/mL (S/N = 3). Finally, due to the presence of overoxidized polypyrrole film characterized by notable rejection properties towards electroactive compounds, a practical application to real sample analysis can be envisaged.

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Section: Scheme 1 Fabrication Steps Of the Biosensor (A) And Currentmentioning

confidence: 99%

Assay of Phospholipase D Activity by an Amperometric Choline Oxidase Biosensor

Ciriello

Guerrieri

2020

Sensors

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“…The electrochemical interface was integrated with poly-m-phenylenediamine membrane. This permselective layer was designed to minimize interfering species [119][120][121]. This is an important factor when developing modern biosensors that will be used directly in complex biological samples.…”

Section: Developing Wearable Integrated Sensors For Multiplex Detectionmentioning

confidence: 99%

Applying Nanomaterials to Modern Biomedical Electrochemical Detection of Metabolites, Electrolytes, and Pathogens

2020

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Personal biosensors and bioelectronics have been demonstrated for use in out-of-clinic biomedical devices. Such modern devices have the potential to transform traditional clinical analysis into a new approach, allowing patients or users to screen their own health or warning of diseases. Researchers aim to explore the opportunities of easy-to-wear and easy-to-carry sensors that would empower users to detect biomarkers, electrolytes, or pathogens at home in a rapid and easy way. This mobility would open the door for early diagnosis and personalized healthcare management to a wide audience. In this review, we focus on the recent progress made in modern electrochemical sensors, which holds promising potential to support point-of-care technologies. Key original research articles covered in this review are mainly experimental reports published from 2018 to 2020. Strategies for the detection of metabolites, ions, and viruses are updated in this article. The relevant challenges and opportunities of applying nanomaterials to support the fabrication of new electrochemical biosensors are also discussed. Finally, perspectives regarding potential benefits and current challenges of the technology are included. The growing area of personal biosensors is expected to push their application closer to a new phase of biomedical advancement.

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“…However, most of them are labor‐intensive, costly and time‐consuming. Amperometric choline oxidase (ChOx) based biosensors hold a great promise for monitoring of choline due to its advantages in simplicity, reliability, rapid response, portability, high sensitivity and economic feasibility [9, 10].…”

Section: Introductionmentioning

confidence: 99%

Choline Oxidase Based Composite ZrO₂@AuNPs with Cu₂O@MnO₂ Platform for Signal Enhancing the Choline Biosensors

et al. 2020

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A novel metal composite material based on zirconium dioxide decorated gold nanoparticles (ZrO2@AuNPs), copper (I) oxide at manganese (IV) oxide (Cu2O@MnO2) and immobilized choline oxidase (ChOx) onto a glassy carbon electrode (GCE) (ChOx/Cu2O@MnO2‐ZrO2@AuNPs/GCE) has been developed for enhancing the electro‐catalytic property, sensitivity and stability of the amperometric choline biosensor. The ChOx/Cu2O@MnO2‐ZrO2@AuNPs/GCE displayed an excellent electrocatalytic response to the oxidation of the byproduct H2O2 from the choline catalyzed reaction, which exhibited a charge transfer rate constant (Ks) of 0.97 s−1, a diffusion coefficient value (D) of 4.50×10−6 cm2 s−1, an electroactive surface area (Ae) of 0.97 cm2 and a surface concentration (γ) of 0.54×10−8 mol cm−2. The modified electrode also provided a wide linear range of choline concentration from 0.5 to 1,000.0 μM with good sensitivity (97.4 μA cm−2 mM−1) and low detection limit (0.3 μM). The apparent Michaelis‐Menten constant was found to be 0.08 mM with Imax of 0.67 μA. This choline biosensor presented high repeatability (%RSD=2.9, n=5), excellent reproducibility (%RSD=2.9, n=5), long time of use (n=28 with %I>50.0 %) and good selectivity without interfering effects from possible electroactive species such as ascorbic acid, aspirin, amoxicillin, caffeine, dopamine, glucose, sucrose and uric acid. This optimal method was successfully applied for choline measurement in prepared human blood samples which demonstrated accurate and excellent reliability in the recovery range from 96.7 to 102.0 %.

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Detection of choline in biological fluids from patients on haemodialysis by an amperometric biosensor based on a novel anti-interference bilayer

Cited by 16 publications

References 50 publications

Assay of Phospholipase D Activity by an Amperometric Choline Oxidase Biosensor

Assay of Phospholipase D Activity by an Amperometric Choline Oxidase Biosensor

Applying Nanomaterials to Modern Biomedical Electrochemical Detection of Metabolites, Electrolytes, and Pathogens

Choline Oxidase Based Composite ZrO₂@AuNPs with Cu₂O@MnO₂ Platform for Signal Enhancing the Choline Biosensors

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Detection of choline in biological fluids from patients on haemodialysis by an amperometric biosensor based on a novel anti-interference bilayer

Cited by 16 publications

References 50 publications

Assay of Phospholipase D Activity by an Amperometric Choline Oxidase Biosensor

Assay of Phospholipase D Activity by an Amperometric Choline Oxidase Biosensor

Applying Nanomaterials to Modern Biomedical Electrochemical Detection of Metabolites, Electrolytes, and Pathogens

Choline Oxidase Based Composite ZrO2@AuNPs with Cu2O@MnO2 Platform for Signal Enhancing the Choline Biosensors

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Product

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Choline Oxidase Based Composite ZrO₂@AuNPs with Cu₂O@MnO₂ Platform for Signal Enhancing the Choline Biosensors