Detection of circulating E/S antigens in the sera of patients with fascioliasis by IELISA: a tool of serodiagnosis and assessment of cure

Abstract

SummaryAn IELISA was developed to evaluate the performance of Fasciola E/S antigens in diagnosis and cure assessment of human Fasciola infection. Twenty patients with acute (prepatent) fascioliasis and another 20 with patent infection were enrolled in the study. Patients were treated with TCZ and followed at 1, 3 and 6 months after therapy. At inspection, the sensitivity of the test to diagnose prepatent cases was 100% compared to 70% for patent infections. There was a gradual decrease of antigenaemia over the follow-up period in acute cases. In chronic cases antigen disappeared from 13 cases (65%) at 1 month; this proportion did not change at 3 or 6 months.keywords Human fascioliasis, E/S antigens, IELISA correspondence A.Y. Shehab,

Diagnostic potential ofFasciola gigantica-derived 14.5 kDa fatty acid binding protein in the immunodiagnosis of bubaline fascioliasis

“…There are three different known approaches for diagnosis of parasitic infections: direct parasitological methods, indirect methods relying on clinical and biochemical assays, and immunological methods measuring the immune response to certain parasitic antigens and/or detecting circulating parasitic antigens (Feldmeier & Poggensee, 1993). Direct parasitological methods usually lack sensitivity and reproducibility and are not reliable (Hillyer, 1988; Shehab et al , 1999; Carnevale et al , 2001). On the other hand, immunodiagnosis of fascioliasis by detecting antibody to the antigen produced from Fasciola worms has several disadvantages.…”