Detection of Circulating Tumor Cells Using a Novel Immunomagnetic Bead Method in Lung Cancer Patients

Ji, Jinling; Jiang, Yahui; Tang, Q Y; He, Xianghuo; Shen, Zuojun; Baiyin, Zhang

doi:10.1002/jcla.21918

Cited by 8 publications

(4 citation statements)

References 22 publications

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“… 14 The isolation and characterization of CTCs remain a technically challenging issue. As the half-life of CTCs is <3 h 15 and CTCs occur at very low concentrations, in the background of millions of normal hematopoietic cells, their identification and capturing require extremely sensitive and specific methods, which usually consist of a combination of enrichment and detection procedures. 16…”

Section: Introductionmentioning

confidence: 99%

Detection of circulating tumor cells in liquid biopsy from Ewing sarcoma patients

Benini¹,

Gamberi²,

Cocchi³

et al. 2018

CMAR

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BackgroundCirculating tumor cells (CTCs) analysis is a promising new diagnostic field to estimate risk and monitor treatment efficacy, metastatic relapse, and progression in cancer patients. The study aim was to isolate and characterize CTCs in blood samples of Ewing sarcoma (ES) patients exploiting two main characteristics: CD99 expression and presence of chromosomal translocations.Materials and methodsThe method isolated CTCs from peripheral blood (PB) of ES patients. Cell-surface CD99 was a useful marker for CTCs determined using immunomagnetic separation with microbeads and CD99 monoclonal antibody. We tested sensitivity and specificity by detecting CTCs in blood collected from healthy donors and randomly during therapy from 18 ES patients. Evidence of CTCs was confirmed by detection of specific molecular markers using quantitative and digital reverse transcription-polymerase chain reaction targeting EWSR1/FLI1 type 1 and type 2 or EWSR1/ETS-related gene transcripts type 1 and type 9e.ResultsFeasibility of finding CTCs in PB of ES patients by immunoseparation with CD99 antibody and magnetic microbeads was demonstrated for the first time. At molecular analysis, three PB specimens tested positive for chimeric EWSR1/FLI1 type 2 and one PB for chimeric EWSR1/FLI1 type 2. CTCs detection was found above a limit of detection of 1 cell/mL of PB.ConclusionCTCs in PB of ES patients can be identified by this method and in ES CTCs analysis can be used as a liquid biopsy approach for prognostic and predictive purposes. The potential clinical implications of CTCs in PB samples detected by the platform for CTC isolation with molecular confirmation during therapy require further evaluation.

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Section: Introductionmentioning

confidence: 99%

Detection of circulating tumor cells in liquid biopsy from Ewing sarcoma patients

Benini¹,

Gamberi²,

Cocchi³

et al. 2018

CMAR

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“…The sampling time was divided into two time points: namely, pre- and post-treatment. Both time points were included in 12 studies [ 9 – 11 , 13 – 21 ], pretreatment alone in 15 studies [ 8 , 12 , 22 – 34 ] and post-treatment alone in 3 studies [ 35 – 37 ]. Nine studies used the reverse-transcriptase polymerase chain reaction (RT-PCR) method, and 21 studies used other methods.…”

Section: Resultsmentioning

confidence: 99%

Clinicopathological and prognostic significance of circulating tumor cells in patients with lung cancer: a meta-analysis

Shen

Cheng

et al. 2017

Oncotarget

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BackgroundThe prognostic significance of circulating tumor cells in patients with lung cancer is controversial. Therefore, we aimed to comprehensively and quantitatively assess the prognostic role of CTCs in patients with lung cancer.MethodsThe relevant literature was searched using PubMed, the Cochrane database and the China National Knowledge Internet database (up to June 2016). Using Review Manager 5.1.2, a meta-analysis was performed using hazard ratio (HR), odds ratio (OR) and 95% confidence interval (CI) as effect values.ResultsThirty studies comprising 2,060 patients with lung cancer were analyzed. The pooled HR values showed that circulating tumor cells were significantly correlated with overall survival (HR =2.63, 95% CI [2.04, 3.39]) and progression-free survival (HR =3.74, 95% CI [2.49, 5.61]) in these patients. Further subgroup analyses were conducted and categorized by sampling time, detection method, and histological type; these analyses showed the same trend. The pooled OR values showed that circulating tumor cells were associated with non small cell lung cancer stage(OR = 2.11, 95% CI [1.42, 3.14]), small cell lung cancer stage (OR = 10.91, 95% CI [4.10, 29.06]), distant metastasis (OR =7.06, 95%CI [2.82, 17.66]), lymph node metastasis (OR =2.31, 95% CI [1.19,4.46]), and performance status(OR =0.42, 95%CI [0.22, 0.78]).ConclusionThe detection of circulating tumor cells in the peripheral blood of patients with lung cancer can be indicative of a poor prognosis.

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“…In this study, only one patient with metastatic disease was positive at baseline for CD99 + t, CD45 − cells. This is likely due to the short half‐life of CTC, which is less than 3 hours 15 . Because they are present at very low concentrations, their detection and collection require highly sensitive and effective methods, which usually include a combination of enrichment and retrieval procedures 12 …”

Section: Discussionmentioning

confidence: 99%

Prognostic value of detection of CD99⁺, CD45⁻ cells in peripheral blood by flow cytometry in children with Ewing sarcoma

Zakzok

El-Shanshory

Zekri

et al. 2021

Pediatric Blood & Cancer

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Background Early detection of metastasis and recurrence of Ewing sarcoma (ES) is important for early management. This work aimed to detect CD99+, CD45− cells in peripheral blood by flow cytometry (FC) before and during chemotherapy and evaluate their prognostic significance. Procedure This prospective cohort study was carried out on 60 children newly diagnosed with ES at Children Cancer Hospital‐Egypt 57357 and 40 healthy children control group. Detection of CD99+, CD45− cells in peripheral blood was accomplished by FC at baseline before treatment and after five cycles of chemotherapy. Samples were classified as positive if they had more than the upper limit of cells observed in the control cases. Correlation between FC results and relapse and overall survival (OS) after one year was performed. Results Median percentage of CD99+, CD45− cells was significantly increased in patients compared with controls (0.002% vs 0%, respectively, P < 0.001). Post‐cycle 5 CD99+, CD45− cells were increased in 12 patients, of them 11 patients’ disease had either relapsed or progressed. Post‐cycle 5 CD99+; CD45− cells had a 73.3% sensitivity and 97.8% specificity for predicting relapse or progression, whereas baseline only had 6.7% sensitivity and 77.8% specificity. The hazard ratio for mortality in the post‐cycle 5 positive group was 18.4 [95% confidence interval (1.86 to 181.46)] times that of the negative group. One year OS was 91.67%. Conclusion Post‐cycle 5 CD99+, CD45− cells in peripheral blood by FC is a strong predictor for relapse, progression, and mortality whereas baseline is a poor predictor in newly diagnosed patients with ES.

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Detection of Circulating Tumor Cells Using a Novel Immunomagnetic Bead Method in Lung Cancer Patients

Abstract: This novel turbulence device provides a new tool allowing for feasible and specific detection of CTCs in lung cancer patients. It is likely clinically useful in diagnosis and monitoring of lung cancer and may have a role in clinical decision making.

Cited by 8 publications

References 22 publications

Detection of circulating tumor cells in liquid biopsy from Ewing sarcoma patients

Detection of circulating tumor cells in liquid biopsy from Ewing sarcoma patients

Clinicopathological and prognostic significance of circulating tumor cells in patients with lung cancer: a meta-analysis

Prognostic value of detection of CD99⁺, CD45⁻ cells in peripheral blood by flow cytometry in children with Ewing sarcoma

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Detection of Circulating Tumor Cells Using a Novel Immunomagnetic Bead Method in Lung Cancer Patients

Abstract: This novel turbulence device provides a new tool allowing for feasible and specific detection of CTCs in lung cancer patients. It is likely clinically useful in diagnosis and monitoring of lung cancer and may have a role in clinical decision making.

Cited by 8 publications

References 22 publications

Detection of circulating tumor cells in liquid biopsy from Ewing sarcoma patients

Detection of circulating tumor cells in liquid biopsy from Ewing sarcoma patients

Clinicopathological and prognostic significance of circulating tumor cells in patients with lung cancer: a meta-analysis

Prognostic value of detection of CD99+, CD45− cells in peripheral blood by flow cytometry in children with Ewing sarcoma

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Prognostic value of detection of CD99⁺, CD45⁻ cells in peripheral blood by flow cytometry in children with Ewing sarcoma