Detection of colonic growth factors using a human colonic carcinoma cell line (LIM 1215)

Abstract

Although the colonic mucosa is one of the most rapidly proliferating epithelial tissues in the body, little is known about the factors that direct this proliferation. In this report we have studied the parameters of both a mitogenic and a clonogenic assay for detecting potential colonic growth factors (CGF). Using a colon carcinoma cell line (LIM1215), which has retained a number of the properties of normal colonic mucosa, we have assayed a range of mitogenic factors for CGF activity. 3H-thymidine incorporation by the LIM1215 cell line was stimulated by low concentrations of epidermal growth factor and basic fibroblast growth factor and, to a lesser extent, by higher concentrations of interleukin-1 and insulin-like growth factor 1. The cells did not respond to a range of other mitogens and lymphokines. Optimal clonogenic response in a soft-agar assay was obtained using a primary pituitary extract.

Mechanisms and experimental and epidemiological evidence relating dietary fibre (non-starch polysaccharides) and starch to protection against large bowel cancer

“…Other SCFA have little effect compared with butyrate (Whitehead et al 1986). The effects of butyrate, however, vary amongst different cell lines; in one line derived from familial adenomatous polyposis, butyrate seemed to select for increased malignancy.…”

“…Butyrate is also a known differentiation-inducing agent, reducing cloning efficiency and increasing the expression of membrane glycoproteins such as alkaline phosphatase (EC 3.1.3.1) and carcino-embryonic antigen in some human colo-rectal cancer cell lines (Chung et al 1985;Whitehead et al 1986). Other SCFA have little effect compared with butyrate (Whitehead et al 1986).…”

Identification of basic fibroblast growth factor sensitivity and receptor and ligand expression in human colon tumor cell lines

“…There was no apparent correlation between bFGF sensitivity and tumor cell aggressiveness or degree of differentiation. Whitehead et al (1990) have described a similar mitogenesis in response to exogenous bFGF in a human colon carcinoma cell line, LIM 1215. Interestingly, LIM 1215 also appears to be a fairly well-differentiated cell line.…”

Expression of PCNA, Basic Fibroblast Growth Factor, FGF-receptor and Vascular Endothelial Growth Factor in Adenomas and Carcinomas of Human Colon.

“…On the other hand, basic fibroblast growth factor (bFGF) is not only growth factor for various cells, but also an angiogenic factor in the growth of solid tumors, which recently has been shown to directly stimulate mitogenic activity in angiogenesis and several kinds of cancer cell lines in vitro [5,17,26]. Vascular endothelial growth factor (VEGF) is secreted by several kind of cells including tumor cells, and it is also known to induce an angiogenic response via a direct effect on endothelial cells [2,15,19,21,22].…”