Detection of colorectal cancer cells in peripheral blood by reverse‐transcriptase polymerase chain reaction for cytokeratin 20

Wyld, David; Selby, Peter J.; Perren, Timothy J.; Jonas, S.; Allen-Mersh, T G; Wheeldon, John; Burchill, Susan A.

doi:10.1002/(sici)1097-0215(19980619)79:3<288::aid-ijc14>3.3.co;2-2

Cited by 31 publications

(44 citation statements)

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“…The mRNAs of CK19 and CK20 have been successfully used to detect CTCs in the peripheral blood of patients with breast cancer, lung cancer, and gastrointestinal carcinoma (Mori et al, 1996;Denis et al, 1997;Peck et al, 1998). Wlyd et al (1998) suggested that CK20 may be a useful target for RT-PCR detection of epithelial-derived cancers than previously described in cytokeratins.…”

Section: Discussionmentioning

confidence: 94%

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Evaluation of MUC1, CK20, and hTERT expression in peripheral blood of gastrointestinal cancer patients in search of diagnostic criteria

Küçükyıldırım¹,

Erdem²,

Saglar³

et al. 2014

Turk J Biol

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The goal of this study was to identify the optimal marker or marker combinations for detection of gastrointestinal malignancies using reverse-transcriptase polymerase chain (RT-PCR) reaction. To detect the presence of tumors, we analyzed mucin 1 (MUC1), cytokeratin 19 (CK19), cytokeratin 20 (CK20), and human telomerase reverse transcriptase (hTERT) mRNA in the peripheral blood of 31 patients with gastrointestinal (esophagus, stomach, and colorectal) cancer and 30 healthy individuals. In RT-PCR analysis of the peripheral blood, 77.4% (24/31), 61.29% (19/31), and 45.16% (14/31) of cancer patients were positive for MUC1, CK20, and hTERT mRNA, respectively. According to our results, any one of these mRNA markers is a predictor of the presence of gastrointestinal tumors (P < 0.001) and colorectal tumors (P < 0.05). However, they did not have predictive potential for presence of metastasis in gastrointestinal tumors. As a result, combination of these 3 tumor-specific mRNA markers would increase the detection rate and may be clinically helpful in predicting tumor presence.

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Section: Discussionmentioning

confidence: 94%

“…Understandably, CKs have served as the most widely used target for detection of metastasis from epithelial-derived tumors in a variety of sites (Wlyd, 1998).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of MUC1, CK20, and hTERT expression in peripheral blood of gastrointestinal cancer patients in search of diagnostic criteria

Küçükyıldırım¹,

Erdem²,

Saglar³

et al. 2014

Turk J Biol

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“…Eight studies were excluded as they reported only on preoperative blood sampling (Bessa et al, 2001;Fujita et al, 2001;Wong et al, 2001;Zhang et al, 2005;Douard et al, 2006;Iinuma et al, 2006;Wang et al, 2006;Friederichs et al, 2007). Nine studies were excluded as they contained data from which outcomes could not be extracted, of which four were excluded as they combined positive results of molecular detection of multiple samples and their effect on prognosis (Hardingham et al, 2000;Guller et al, 2002;Bosch et al, 2003;Koyanagi et al, 2008), and five were excluded as it was impossible to extract the data for outcomes of interest (White and Griffiths, 1976;Funaki et al, 1998;Wyld et al, 1998;Conzelmann et al, 2005;Wang et al, 2007). Five studies were excluded as they were reviews or systematic reviews, which did not report on prognostic outcomes (Tsavellas et al, 2001;Sleijfer et al, 2007;Riethdorf et al, 2008;Sergeant et al, 2008;Tsouma et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

Prognostic significance of circulating tumour cells following surgical resection of colorectal cancers: a systematic review

et al. 2010

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Background: The role of adjuvant chemotherapy after resection of colorectal cancers (CRCs) is well understood for patients with stage-I or stage-III disease. Its efficacy for those with stage-II disease remains much less clear. Many investigators have sought to identify prognostic markers that might clarify which patients have the highest risk of recurrence and would, therefore, be most likely to benefit from chemotherapy. This systematic review examines evidence for the use of peripherally sampled, circulating tumour cells (CTCs) as such a prognostic marker. Methods: A comprehensive literature search was used to identify studies reporting on the significance of CTCs in the postoperative blood of CRC patients. Results: Fourteen studies satisfied the inclusion criteria. Six of the nine studies that took blood samples 24 h or more postoperatively found detection of postoperative CTCs to be an independent predictor of cancer recurrence. Conclusion: The presence of CTCs in peripheral blood at least 24 h after resection of CRCs is an independent prognostic marker of recurrence. Further studies are needed to clarify the optimal time point for blood sampling and determine the benefit of chemotherapy in CTC-positive patients with stage-II disease.

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“…tyrosinase or prostatespecific antigen (PSA) (Gomella et al, 1997;Ghossein et al, 1998), or lineage-specific mRNAs like the cytokeratins that characterize cells of epithelial origin. Assays designed for the detection of epithelial cells mainly use mRNA of the cytokeratins 18, 19 and 20 Tschentscher et al, 1997;Futamura et al, 1998;Wyld et al, 1998).…”

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confidence: 99%

Detection of micrometastasis by cytokeratin 20 RT-PCR is limited due to stable background transcription in granulocytes

et al. 1999

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SummaryThe reverse transcription polymerase chain reaction (RT-PCR) amplification of cytokeratin 20 (CK20) mRNA is considered a promising candidate method for the detection of circulating tumour cells in bone marrow and peripheral blood of cancer patients. In this study we have investigated the diagnostic specificity of the CK20 mRNA detection in samples from healthy donors (HD; n = 33), intensive care units patients (ICU; n = 20) and bone marrow obtained from patients suffering from chronic inflammatory diseases (CID; n = 14). RNAs purified from stabilized lysates showed positive results in 24% of the HD group (8/33), 35% of the ICU group (8/20) and in 40% of the CID group (5/14). The use of Ficoll gradients to separate nucleated cells completely restored the specificity of this CK20 RT-PCR assay. The CK20-expressing cells are positively identified to belong to the granulocyte fraction of leucocytes, which appear to express the gene on a background level. Our results demonstrate for the first time that CK20 mRNA expression is not limited to epithelium. Its occurrence in normal granulocytes has to be considered in tests designed to detect circulating cancer cells or micrometastases.

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Detection of colorectal cancer cells in peripheral blood by reverse‐transcriptase polymerase chain reaction for cytokeratin 20

Cited by 31 publications

References 0 publications

Evaluation of MUC1, CK20, and hTERT expression in peripheral blood of gastrointestinal cancer patients in search of diagnostic criteria

Evaluation of MUC1, CK20, and hTERT expression in peripheral blood of gastrointestinal cancer patients in search of diagnostic criteria

Prognostic significance of circulating tumour cells following surgical resection of colorectal cancers: a systematic review

Detection of micrometastasis by cytokeratin 20 RT-PCR is limited due to stable background transcription in granulocytes

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