Detection of cow’s milk in Shaanxi goat’s milk with an ELISA assay

Song, Haiyan; Xue, Haiyan; Yan, Han

doi:10.1016/j.foodcont.2010.11.019

Cited by 45 publications

(22 citation statements)

References 22 publications

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“…This method identifies molecular masses to differentiate between proteins in the milk of cow and goat. Detection of addition of cow milk to goat and ewe milk has been described by (Abrantes et al 2014;Romero, PerezAndújar, Olmedo, & Jiménez, 1996;Song, Xue, & Han, 2011). Since, PDO (Protected Denomination of Origin) cheeses are products of high commercial value confined according to legislative and proper labelling rules, different analytical techniques have been developed to evaluate the authenticity.…”

Section: Milk Adulteration With Milk From Different Sourcesmentioning

confidence: 99%

Common milk adulteration and their detection techniques

2016

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Food adulteration is a global concern and developing countries are at higher risk associated with it due to lack of monitoring and policies. However, this is one of the most common phenomena that has been overlooked in many countries. Unfortunately, in contrast to common belief, milk adulterants can pose serious health hazards leading to fatal diseases. This paper presents a detailed review of common milk adulterants as well as different methods to detect the adulterants both qualitatively and quantitatively. This study is organized to be an 'adulterant based' study instead of 'techniques based' one, where qualitative detection for most of the common adulterants are enlisted and quantitative detection methods are limited to few major adulterants of milk. Apart from regular techniques, recent development in these detection techniques have also been reported. Nowadays milk is being adulterated in more sophisticated ways that demands for cutting edge research for the detection of the adulterants. This review intends to contribute towards the common knowledge base regarding possible milk adulterants and their detection techniques.

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Section: Milk Adulteration With Milk From Different Sourcesmentioning

confidence: 99%

Common milk adulteration and their detection techniques

2016

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“…This condition makes many traders adulterating goat's milk with cow milk to gain more profit. In other countries, adulteration of sheep milk or goat milk with cow milk was also practiced [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…Most of the methods were based on a laboratory intensive procedure such as ELISA, PCR, spectroscopy, 2 Journal of Sensors chromatography, and HPLC [6][7][8][9][10][11][12][13]. Those methods were successfully developed to identify milk adulteration.…”

Section: Introductionmentioning

confidence: 99%

Development of QCM Biosensor with Specific Cow Milk Protein Antibody for Candidate Milk Adulteration Detection

et al. 2016

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Adulteration of goat milk is usually done using cow's milk product. Cow milk is used as it is widely available and its price is cheaper compared to goat milk. This paper shows a development of candidate tools for milk adulteration using cow milk. A quartz crystal microbalance immunosensor was developed using commercial crystal resonator and polyclonal antibody specific to cow milk protein. A specific protein at 208 KDa is found only in cow milk and does not exist in goat milk. The existence of this protein can be used as an indicator of cow milk content in a target solution. To detect the PSS 208 kDa protein, antibody specific to the PSS 208 was developed. The purified antibody was immobilized on top of the sensor surface on a polystyrene layer. The fraction of the immobilized antibody on the sensor was found at 1.5% of the given antibody. Using a static reaction cell, the developed immunosensor could detect the specific cow milk protein in buffer solution. The detection limit is 1 ppm. A linear relationship between frequency change and specific protein of cow milk concentration is found from a concentration of 1 ppm to 120 ppm.

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“…Immunoassays based on antibody are sensitive and high throughput procedure for quantitative detection and also successfully applied to measurement of milk protein content, such as enzyme-linked immunosorbent assay (ELISA) for αs1-CN (Black and Reynolds, 1998) and β-CN (Song et al, 2011;Zhou et al, 2013), immunomagnetic beads-based immunoassay for β-CN , and nephelometric immunoassay for αs-CN and κ-CN (Collard-Bovy et al, 1991).…”

Section: Introductionmentioning

confidence: 99%