Detection of Dengue Fever Virus Serotype – 4 by using One-Step Real-Time RT-PCR in Hodeidah, Yemen

Alahdal, Murad; Al-Shabi, Jamal; Ogaili, Mahmoud; Abdullah, Qais; Alghalibi, Said; Jumaan, Aisha O.; AL-Kamarany, Mohammed Amood

doi:10.9734/bmrj/2016/24380

Cited by 9 publications

(10 citation statements)

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“…All three dengue viral serortyp1-3 were reported in Yemen but type 2 of the Dengue virus was the most predominant serotype in Yemen 12 . Interestingly DENV serotype 4 was identified and confirmed the first time in Al-Hudidah-Yemen by Alahdal et al, 13 . In the Taiz governorate, the infection rate varied from year to year.…”

Section: Introductionsupporting

confidence: 58%

Seroprevalence of Dengue Fever Virus Among Suspected Patients in Taiz Governorate-Yemen

et al. 2020

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Background: Dengue Fever virus (DENV) considers one of the most important mosquito-borne viral diseases in the world and it is endemic in more than 100 countries. Objective: This study aimed to determine the seroprevalence of DENV infection among suspected patients and to investigate some associated risk factors with dengue fever infection in Taiz governorate, Yemen. Methods: This study was cross-sectional, descriptive, and experimental, combining the use of a structured questionnaire and analysis of serum samples obtained from 300 suspected patients attending at many hospital and clinic centers in Taiz during the period from July to November 2016. The serum samples were tested for anti-dengue immunoglobulin (IgM) and (IgG) by Enzyme-linked Immunosorbent Assay (ELIZA). Results: Out of 300 suspected febrile cases, it was found that 49(16.3%), 68(22.7%), and 17(5.7%) cases were showed positive for the IgM, IgG, and both IgM and IgG antibodies, respectively, while 166 (55.3%) cases were negative. The incidence rate was more in males than in females. The most affected age group with dengue fever infection were (21–30) years. Dengue Fever was more frequent among patients coming from the urban area, having secondary school, and low-income status people. Also, there was statistical significant between DENV infections with a place of residencyand gender (P<0.05) and not-statistical significance between DENV infections and other factors (P>0.05). Conclusion: Taiz governorate become one of the endemic governorates in Yemen particularly the Taiz city which should be brought to the attention of public health authorities Peer Review History: Received: 5 September 2020; Revised: 15 October; Accepted: 26 October, Available online: 15 November 2020 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.0/10 Reviewer(s) detail: Dr. Rola Jadallah, Arab American University, Palestine, rola@aauj.edu Dr. Dulger Gorkem, Duzce University, Turkey, gorkemdulger@yandex.com Comments of reviewer(s): Similar Articles: QUALITY ASSESSMENT OF DIFFERENT BRANDS OF PARACETAMOL TABLETS IN YEMENI MARKET

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Section: Introductionsupporting

confidence: 58%

Seroprevalence of Dengue Fever Virus Among Suspected Patients in Taiz Governorate-Yemen

et al. 2020

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“…Before the current civil war began, all 4 DENV serotypes were present in Yemen, but only DENV-2 ( 10 ), DENV-3 ( 12 , 13 ), and DENV-4 ( 14 ) have been documented to cause outbreaks. Using partial nonstructural protein 1 gene sequences, Ciccozzi et al identified the DENV-2 Cosmopolitan genotype as the virus that caused the outbreak in Hodeidah during 2012 ( 15 ).…”

Section: Discussionmentioning

confidence: 99%

Dengue Outbreak during Ongoing Civil War, Taiz, Yemen

Alghazali¹,

Teoh²,

Loong³

et al. 2019

Emerg. Infect. Dis.

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“…Different studies showed increased frequency in male patients [10,15,17] including a study done in Hadhramaut 2010 [8]. But our results showed the reverse as male-to-female ratio was 0.9:1.…”

Section: Discussionmentioning

confidence: 39%

“…DENV-4 was the predominant serotype at 31.88% followed by DENV-2 at 23.18%, DENV-3 at 20.28%, and DENV-1 at 10.14% [15]. DENV-1 was confirmed in Alhodeidah governorate, whereas DENV-3 was confirmed in Hadhramaut governorate in the 2010 outbreak [7,8].…”

Section: Discussionmentioning

confidence: 91%

Clinical spectrum of dengue fever in hospitalized children in Mukalla city, Yemen after the attack of Chapala cyclone Nov. 2015

Dahman¹,

Hatem²

2017

IJMDC

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Detection of Dengue Fever Virus Serotype – 4 by using One-Step Real-Time RT-PCR in Hodeidah, Yemen

Cited by 9 publications

References 0 publications

Seroprevalence of Dengue Fever Virus Among Suspected Patients in Taiz Governorate-Yemen

Seroprevalence of Dengue Fever Virus Among Suspected Patients in Taiz Governorate-Yemen

Dengue Outbreak during Ongoing Civil War, Taiz, Yemen

Clinical spectrum of dengue fever in hospitalized children in Mukalla city, Yemen after the attack of Chapala cyclone Nov. 2015

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