Detection of Dengue Virus NS1 Antigen in Infected Aedes aegypti Using a Commercially Available Kit

Abstract

Abstract. Epidemic dengue has emerged throughout the tropical world. In the continued absence of a vaccine against dengue virus (DENV), mosquito vector surveillance and control programs are essential to reduce human infections. An effective test to detect DENV in infected mosquitoes would be a valuable addition to the surveillance effort. We investigated DENV detection in infected Aedes aegypti using a commercially available DENV non-structural protein 1 (NS1) ELISA kit (Platelia Dengue NS1 Ag), and by reverse transcription-polymerase chain reaction (RT-PCR) and virus isolation assays. The DENV-infected mosquitoes were subjected to field-relevant conditions and assayed individually and pooled with uninfected mosquitoes. Overall, DENV NS1 antigen was detected in 98% of infected mosquitoes/ pools versus 79% for RT-PCR and 29% for virus isolation. Our results indicate that NS1 is an excellent analyte for detection of DENV in Ae. aegypti and that the tested NS1 antigen kit provides a sensitive, rapid, and convenient test for DENV surveillance in mosquitoes.

Experimental Inoculation of Artibeus jamaicensis Bats with Dengue Virus Serotypes 1 or 4 Showed No Evidence of Sustained Replication

“…Mosquitoes were maintained under controlled conditions, and 7 days after the blood meal, each pool was macerated in phosphate buffered saline, the mixture clarified by low speed centrifugation and the clarified supernatant tested for the presence of soluble NS1 by a commercial enzyme-linked immunosorbent assay (ELISA). 26 Mosquito strains. Aedes aegypti mosquitoes (Rockefeller strain), were maintained under controlled conditions at 26 to 28 C and 70-80% relative humidity with 12-h/12-h light-dark cycle at the insectary of the National Public Health Institute (INSP), Cuernavaca, Mexico.…”

Improving dengue viral antigens detection in dengue patient serum specimens using a low pH glycine buffer treatment

“…3 Maximal extracellular virus titers are not usually observed until approximately 24 hours postinfection. In DENV-infected mosquitos 4 or mammalian cell culture, a soluble hexameric form of NS1 protein is secreted into the cultured supernatant in a glycosylation-dependent fashion and in free-form association with virion particles, 5,6 and the levels of secreted NS1 (sNS1) correlate with infectious virus titers. 7 The circulating sNS1 during the acute phase of the disease is higher in patients' serum specimens experiencing primary rather than secondary infection.…”

Anti-Viral Effects of Medicinal Plants in the Management of Dengue: A Systematic Review

“…Dengue is considered one of the most important of these diseases (Gubler, 2002;Voge et al, 2013WHO, 2016. Dengue is transmitted by the bite of the Aedes aegypti mosquito infected with one of the four dengue virus (DENV 1 -4) serotypes (genus Flavivirus, family Flaviviridae) (Voge et al, 2013;Halstead, 2007;Gubler, 2002;Guzman and Isturiz, 2010;WHO, 2016). Infection with one DENV serotype produces only specific antibody against that serotype.…”