Detection of dengue viruses in field-caught Aedes aegypti (Diptera: Culicidae) in Maracay, Aragua state, Venezuela by type-specific polymerase chain reaction

Urdaneta, Ludmel; Herrera, Flor; Pernalete, Martha; Zoghbi, Normig; Rubio-Palis, Yasmín; Barrios, Roybel; Rivero, José; Comach, Guillermo; Jiménez, Matilde; Salcedo, M.

doi:10.1016/j.meegid.2004.09.004

Cited by 58 publications

(56 citation statements)

References 12 publications

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“…In contrast, studies using adult females collected in the fi eld have obtained high MIRs, such as the 8.52% detected by Lourenço-de-Oliveira et al, 10 the 16‰ detected by Urdaneta et al, 17 who collected adults from the homes of persons infected with dengue and neighboring households, and the 57.6 ‰ detected by Chow et al 1 Therefore, screening for presence of virus in Ae. aegypti larvae is not the most adequate method for predicting epidemics, given its low rate of infection when compared to the screening of adult females.…”

Section: Discussionmentioning

confidence: 97%

“…18 Detection and serotyping of dengue virus by reverse transcription followed by polymerase chain reaction (RT-PCR) using material from clinical or vector samples is a powerful tool for epidemiological surveillance. 1,10,17 Presence of virus in mosquitoes collected in the fi eld allows for the detection of epidemics from six to eight weeks in advance of their onset. 1,17 The aim of the present study was to identify the presence of dengue virus in Aedes aegypti larvae and to associate presence of vector with rainfall and number of cases of dengue in the period.…”

Section: Introductionmentioning

confidence: 99%

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Vírus dengue em larvas de Aedes aegypti e sua dinâmica de infestação, Roraima, Brasil

Zeidler¹,

Acosta²,

Barrêto³

et al. 2008

Rev. Saúde Pública

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OBJECTIVE:To detect the presence of dengue virus in larval forms of Aedes aegypti and to associate vector presence with rainfall and incidence of disease. METHODS:Eighteen households were randomly selected for egg collection in a neighborhood of the city of Boa Vista, Roraima, in Northern Brazil. Two oviposition traps were installed per home, and removed after one week. This was repeated on a monthly basis between November 2006 and May 2007. Trap positivity rate and egg density were calculated. Following the eclosion of 1,422 eggs, 44 pools of at least 30 larvae each were formed, which were evaluated for presence of dengue virus using RT-PCR and hemi-nested PCR. Dengue incidence rates in the period were correlated with rainfall rates. The association between these two variables and the number of eggs collected was determined using Pearson correlation. RESULTS:None of the pools tested positive for presence of dengue virus, despite the high incidence of dengue in the neighborhood during the studied period. The density of Ae. aegypti increased with rainfall, but was not correlated with incidence of dengue. CONCLUSIONS:The results suggest that transovarial transmission of dengue virus in mosquitoes occurs at a very low frequency, and therefore virus persistence in urban settings may not depend on such transmission. The mosquito population increased during the rainy season due to increased formation of breeding sites; the lack of correlation with incidence of dengue may be due to underestimation of incidence data during epidemics.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Vírus dengue em larvas de Aedes aegypti e sua dinâmica de infestação, Roraima, Brasil

Zeidler¹,

Acosta²,

Barrêto³

et al. 2008

Rev. Saúde Pública

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“…However, the introduction of DENV3, found the whole population to be susceptible to it, which perhaps contributed to dispersion, thus becoming easier to be detected and isolated. In fact, earlier experiments showed that when a new dengue serotype is introduced into a community, occurs a natural decrease of circulation of other previously existing dengue serotypes (WHO 1997, Rigau-Pérez et al 2002, Urdaneta et al 2005. Moreover, the official data of the Ministry of Health, confirmed these findings, since only DENV3 isolations were officially recorded in 2003 (MS 2003).…”

Section: Discussionmentioning

confidence: 88%

“…The RT-PCR technique for detecting dengue virus in mosquito tissues has been performed in several regions of the world since the 1990s (Chungue et al 1993, Chow et al 1998, Harris et al 1998, Romero-Vivas et al 2000, Kow et al 2001, Chung & Pang 2002, Urdaneta et al 2005. Mostly of these studies were conducted in Asia, where the four serotypes occur and the incidence of DHF/DSS is very high.…”

Section: Discussionmentioning

confidence: 99%

“…Besides preventing the DENV3 dispersion in the population, the epidemiological surveillance must be able to monitor the circulation of other serotypes and also be able to early detect the introduction of the DENV4 that has been already isolated in Venezuela (Urdaneta et al 2005), a bordering country with terrestrial connection to Manaus through a highway. Figueiredo LTM 2003.…”

Section: Discussionmentioning

confidence: 99%

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Detection of dengue virus serotype 3 by reverse transcription-polymerase chain reaction in Aedes aegypti (Diptera, Culicidae) captured in Manaus, Amazonas

Pinheiro

Tadei

Barros

et al. 2005

Mem. Inst. Oswaldo Cruz

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Genetic deviation in geographically close populations of the dengue vector Aedes aegypti (Diptera: Culicidae): influence of environmental barriers in South India

et al. 2015

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Mosquitoes are vectors of devastating pathogens and parasites, causing millions of deaths every year. Dengue is a mosquito-borne viral infection found in tropical and subtropical regions around the world. Recently, dengue transmission has strongly increased in urban and semiurban areas, becoming a major international public health concern. Aedes aegypti (Diptera: Culicidae) is a primary vector of dengue. Shedding light on genetic deviation in A. aegypti populations is of crucial importance to fully understand their molecular ecology and evolution. In this research, haplotype and genetic analyses were conducted using individuals of A. aegypti from 31 localities in the north, southeast, northeast and central regions of Tamil Nadu (South India). The mitochondrial DNA region of cytochrome c oxidase 1 (CO1) gene was used as marker for the analyses. Thirty-one haplotypes sequences were submitted to GenBank and authenticated. The complete haplotype set included 64 haplotypes from various geographical regions clustered into three groups (lineages) separated by three fixed mutational steps, suggesting that the South Indian Ae. aegypti populations were pooled and are linked with West Africa, Columbian and Southeast Asian lineages. The genetic and haplotype diversity was low, indicating reduced gene flow among close populations of the vector, due to geographical barriers such as water bodies. Lastly, the negative values for neutrality tests indicated a bottle-neck effect and supported for low frequency of polymorphism among the haplotypes. Overall, our results add basic knowledge to molecular ecology of the dengue vector A. aegypti, providing the first evidence for multiple introductions of Ae. aegypti populations from Columbia and West Africa in South India.

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Detection of dengue viruses in field-caught Aedes aegypti (Diptera: Culicidae) in Maracay, Aragua state, Venezuela by type-specific polymerase chain reaction

Cited by 58 publications

References 12 publications

Vírus dengue em larvas de Aedes aegypti e sua dinâmica de infestação, Roraima, Brasil

Vírus dengue em larvas de Aedes aegypti e sua dinâmica de infestação, Roraima, Brasil

Detection of dengue virus serotype 3 by reverse transcription-polymerase chain reaction in Aedes aegypti (Diptera, Culicidae) captured in Manaus, Amazonas

Genetic deviation in geographically close populations of the dengue vector Aedes aegypti (Diptera: Culicidae): influence of environmental barriers in South India

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