Detection of disseminated tumour cells in blood and bone marrow samples of patients undergoing hepatic resection for metastasis of colorectal cancer

Vlems, F. A.; Diepstra, J. Heleen S.; Punt, Cornelis J.A.; Ligtenberg, M. J. L.; Cornelissen, Ine M. H. A.; Krieken, J.H.J.M. van; Wobbes, Th.; Muijen, G.N.P. van; Ruers, Theo J.M.

doi:10.1002/bjs.4161

Cited by 34 publications

(22 citation statements)

References 33 publications

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“…Whether these cells are responsible for a later relapse must be determined since no differences could be obtained when the detection rate of CK + cells in the survivor group was compared with those patients who died. Similar conclusions were demonstrated by Velms et al, who showed no correlation between CK-20 positivity in blood or bone marrow and later extrahepatic tumor recurrence (26).…”

Section: Discussionsupporting

confidence: 89%

Tumor cell dissemination in colon cancer does not predict extrahepatic recurrence in patients undergoing surgery for hepatic metastases

Schoppmeyer

Frühauf²,

Oldhafer³

et al. 2006

Oncol Rep

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Abstract. Patients undergoing resection of hepatic metastases of colorectal cancer have a high risk of extrahepatic recurrence, most likely caused by early tumor cell dissemination or the manipulation of liver tumors during surgical resection. Using immunocytochemistry, we studied 47 patients for cytokeratin (CK)-positive (+) cells in: a) bone marrow (BM) samples to determine whether tumor cell dissemination had already occurred before surgery; and b) blood samples directly taken from the hepatic vein before and during surgery of liver metastases. In addition, normal and malignant liver tissues were evaluated for markers known to be involved in tumor progression and metastasis [urokinase plasminogen activator (uPA), Her-2/neu, epidermal growth factor receptor (EGF-R)] using sandwich enzyme immunoassays. CK + cells were detected in the BM of 26/47 patients (55%), in blood samples of 14/47 patients (30%) before surgery and 11/47 patients (23%) during surgery with a median detection rate of 1 (range, 1-14) CK + cell per 4x10 6 MNC. No CK + cells were found in 15/47 patients (32%) in any sample studied. Tumor tissue was obtained from 32/47 patients and normal liver tissue from 24/32 patients. While no differences were found for EGF-R and Her-2/neu, a 9-fold higher expression of uPA could be demonstrated in tumor tissue of 20/32 patients (63%) compared to normal liver tissue. When all obtained results were correlated with clinical outcome, neither the detection of CK + cells nor the expression pattern in the tumor tissue, or the combination of both, was predictive for extrahepatic recurrence or overall survival after a mean observation time of 43 months (range, 26-54 months). Although uPa is overexpressed in liver metastases of colorectal cancer, and dissemination of CK + cells during surgery of these metastases is a frequent event in colon cancer, these findings do not predict extrahepatic recurrence. Further characterization of single cells, especially those spread during surgery, will help to identify those patients with an increased risk of later relapse.

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Section: Discussionsupporting

confidence: 89%

Tumor cell dissemination in colon cancer does not predict extrahepatic recurrence in patients undergoing surgery for hepatic metastases

Schoppmeyer

Frühauf²,

Oldhafer³

et al. 2006

Oncol Rep

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“…Furthermore, recurrence may also develop from circulating tumour cells that are shed into the circulation during surgery [7,8] . In the past decades, many prognostic factors of recurrence and survival after hepatectomy for metastatic colorectal cancer have been documented, including both preoperative [9,10] and peri-operative variables [11][12][13][14] .…”

Section: Introductionmentioning

confidence: 99%

Prolonged portal triad clamping during liver surgery for colorectal liver metastases is associated with decreased time to hepatic tumour recurrence

Nijkamp

Bilt

Snoeren

et al. 2010

European Journal of Surgical Oncology (EJSO)

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. M A N U S C R I P T A C C E P T E D ARTICLE IN PRESSThe aim of this study was to evaluate the oncological outcome of portal triad clamping during hepatectomy in colorectal cancer patients.METHODS: 160 patients with colorectal liver metastases underwent a partial hepatectomy with curative intent. Data were collected in a prospective database and were retrospectively analyzed for time to liver recurrence (TTLiR) and time to overall recurrence (TTR). The prognostic significance of portal triad clamping of any type and severe ischemia due to prolonged portal triad clamping was determined by Cox regression models.RESULTS: TTLiR was reduced after clamping of any type, although not statistically significant (p=0.061). Severe ischemia due to prolonged portal triad clamping significantly decreased TTLiR (p=0.022), but not TTR. Furthermore, severe ischemia independently predicted TTLiR in a multivariable analysis (p= 0.038).CONCLUSIONS: Severe ischemia due to prolonged portal triad clamping during hepatic resection for colorectal liver metastases appears to be associated with decreased TTLiR.Further research remains necessary to determine the causative effect of prolonged vascular clamping on liver tumour recurrence.

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“…mRNA based studies frequently use the same markers and, in addition, guanylcyclase, which is expressed at the apical brush border of colonic cells [14][15][16][17]. To increase the sensitivity and to avoid false positive results, it has been proposed that multiple markers should be used simultaneously [14,[18][19][20][21]. Screening studies for disseminated colorectal cancer cells in the liver based on aberrant DNA have also been used and they mostly evaluate point mutations of K-ras and p53 and fragment length polymorphism.…”

Section: Colorectal Cancer and Metastasismentioning

confidence: 98%

The Colorectal Cancer Initiating Cell: Markers and Their Role in Liver Metastasis

Zöller

Jung

2011

Liver Metastasis: Biology and Clinical Management

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Colorectal cancer is one of the leading cancers in the Western world with a steadily increasing incidence. The most common site of metastasis is the liver, where the appearance of metastases strongly correlates with survival time and survival rates. After a brief overview on colorectal cancer and the metastatic process, this chapter will focus on the possible impact of the cancer initiating cell and the markers EpCAM and CD44 on metastasis formation. This will include a discussion on the importance of the tumor matrix and of exosomes in angiogenesis and in preparing the metastatic niche.

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Detection of disseminated tumour cells in blood and bone marrow samples of patients undergoing hepatic resection for metastasis of colorectal cancer

Cited by 34 publications

References 33 publications

Tumor cell dissemination in colon cancer does not predict extrahepatic recurrence in patients undergoing surgery for hepatic metastases

Tumor cell dissemination in colon cancer does not predict extrahepatic recurrence in patients undergoing surgery for hepatic metastases

Prolonged portal triad clamping during liver surgery for colorectal liver metastases is associated with decreased time to hepatic tumour recurrence

The Colorectal Cancer Initiating Cell: Markers and Their Role in Liver Metastasis

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