Detection of DNA damage in pigeon erythrocytes using a chromatin dispersion assay

Cortés‐Gutiérrez, Elva I.; García-Salas, Juan A.; Dávila-Rodríguez, Martha I.; Ceyca-Contreras, Juan P; Cortez-Reyes, Michel; Fernández, José Luis Fernández; Gosálvez, Jaime

doi:10.1080/15376516.2019.1701596

Cited by 8 publications

(3 citation statements)

References 54 publications

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“…DNA damage is frequently observed in apoptotic cells ( Wu & Crowe, 2020 ). When apoptosis occurs, DNA breakage, nuclear chromatin agglutination, and nuclear membrane rupture are usually induced ( CortésGutiérrez et al., 2020 ). ROS accumulation has been implicated in causing DNA damage and apoptosis in diverse cancers ( Barzilai & Yamamoto, 2004 ; Guachalla & Rudolph, 2010 ; Guo et al, 2014 ; Sadiq, 2023 ).…”

Section: Resultsmentioning

confidence: 99%

Phenethyl isothiocyanate inhibits the carcinogenic properties of hepatocellular carcinoma Huh7.5.1 cells by activating MAPK/PI3K-Akt/p53 signaling pathways

Du,

Zhang,

Chen

et al. 2024

PeerJ

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Background Hepatocellular carcinoma (HCC) is an aggressive malignancy with limited effective treatment options. Phenethyl isothiocyanate (PEITC) is a bioactive substance present primarily in the cruciferous vegetables. PEITC has exhibited anti-cancer properties in various cancers, including lung, bile duct, and prostate cancers. It has been demonstrated that PEITC can inhibit the proliferation, invasion, and metastasis of SK-Hep1 cells, while effectively inducing apoptosis and cell cycle arrest in HepG2 cells. However, knowledge of its anti-carcinogenic effects on Huh7.5.1 cells and its underlying mechanism remains elusive. In the present study, we aim to evaluate the anti-carcinogenic effects of PEITC on human HCC Huh7.5.1 cells. Methods MTT assay and colony formation assay was performed to investigate the anti-proliferative effects of PEITC against Huh7.5.1 cells. The pro-apoptosis effects of PEITC were determined by Annexin V-FITC/PI double staining assay by flow cytometry (FCM), mitochondrial transmembrane potential (MMP) measurement, and Caspase-3 activity detection. A DAPI staining and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay was conducted to estimate the DNA damage in Huh7.5.1 cells induced by PEITC. Cell cycle progression was determined by FCM. Transwell invasion assay and wound healing migration assay were performed to investigate the impact of PEITC on the migration and invasion of Huh7.5.1 cells. In addition, transcriptome sequencing and gene set enrichment analysis (GSEA) were used to explore the potential molecular mechanisms of the inhibitory effects of PEITC on HCC. Quantitative real-time PCR (qRT-PCR) analysis was performed to verify the transcriptome data. Results MTT assay showed that treatment of Huh7.5.1 cells with PEITC resulted in a dose-dependent decrease in viability, and colony formation assay further confirmed its anti-proliferative effect. Furthermore, we found that PEITC could induce mitochondrial-related apoptotic responses, including a decrease of mitochondrial transmembrane potential, activation of Caspase-3 activity, and generation of intracellular reactive oxygen species. It was also observed that PEITC caused DNA damage and cell cycle arrest in the S-phase in Huh7.5.1 cells. In addition, the inhibitory effect of PEITC on the migration and invasion ability of Huh7.5.1 cells was assessed. Transcriptome sequencing analysis further suggested that PEITC could activate the typical MAPK, PI3K-Akt, and p53 signaling pathways, revealing the potential mechanism of PEITC in inhibiting the carcinogenic properties of Huh7.5.1 cells. Conclusion PEITC exhibits anti-carcinogenic activities against human HCC Huh7.5.1 cells by activating MAPK/PI3K-Akt/p53 signaling pathways. Our results suggest that PEITC may be useful for the anti-HCC treatment.

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Section: Resultsmentioning

confidence: 99%

Phenethyl isothiocyanate inhibits the carcinogenic properties of hepatocellular carcinoma Huh7.5.1 cells by activating MAPK/PI3K-Akt/p53 signaling pathways

Du,

Zhang,

Chen

et al. 2024

PeerJ

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“…Recently, in a study with pigeons with the DBD-FISH technique, the presence of substantial damage in both MNs and buds due to the effect of physical and chemical genotoxic agents has been proven (Cortés-Gutiérrez et al 2019).…”

Section: Discusionmentioning

confidence: 99%

“…Regarding the selection of the house sparrow as a bioindicator of environments, it can be stated that given their peridomestic habits they represent suitable organisms when it is intended to evaluate the genotoxic effects of atmospheric pollutants affecting human populations (Cortés-Gutiérrez et al 2019). The bioaccumulation of heavy metals in sparrow tissues suggested considering the house sparrow as an indicator of environmental pollution (Millaku et al 2015).…”

Section: Discusionmentioning

confidence: 99%

Erythrocyte micronucleus cytome assay in Passer domesticus and environmental remote sensing for inferring the quality of wild, rural, and urban areas

Mendez

Quero²,

Gorla³

2022

Environ Monit Assess

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The study of biomarkers in free-living birds can help indicate the degree of contamination in distinct environments. In addition, these environments can be characterized through the information provided by satellite images. The objectives of the present study were to analyze the types and quantity of cytogenetic biomarkers in Passer domesticus (sparrow) from three different environments; wild, rural and urban; and to analyze them in the context of land use and anthropogenic actions. Five thousand erythrocytes per bird were analyzed for the following nuclear alterations (NA): micronuclei (MN), nuclear buds, notched nuclei, binucleated cells, nucleoplasmic bridges, nuclear tails, peripheral nuclei and enucleated cells. In the study, wild birds exhibited five types of NA, seven types were found in rural birds; and all types were encountered in urban birds. The only NA that exceeded a frequency of 2 NA/ 1000 erythrocytes were peripheral nuclei in birds from the rural and urban sites, the latter environment characterized by 87% urban soil and air pollution. The highest frequencies of MN, peripheral nuclei and enucleated erythrocytes were recorded in sparrows from the rural site (p≤0.05).This area had been sprayed with chlorpyrifos 48%, the most widely used organophosphate in the region. Sparrows from the wild site, made up of 100% native forest, had higher frequencies of notched nuclei (p≤0.05). A precedent is set for the use of environmental remote sensing in a complementary manner with cytogenetic biomarker studies in birds for a joint analysis in environmental assessment.

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Chromatin Dispersion Test to Asses DNA Damage in Cervical Epithelial Cells

Cortés‐Gutiérrez

Fernández

Dávila-Rodríguez

et al. 2022

Methods in Molecular Biology

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Detection of DNA damage in pigeon erythrocytes using a chromatin dispersion assay

Cited by 8 publications

References 54 publications

Phenethyl isothiocyanate inhibits the carcinogenic properties of hepatocellular carcinoma Huh7.5.1 cells by activating MAPK/PI3K-Akt/p53 signaling pathways

Phenethyl isothiocyanate inhibits the carcinogenic properties of hepatocellular carcinoma Huh7.5.1 cells by activating MAPK/PI3K-Akt/p53 signaling pathways

Erythrocyte micronucleus cytome assay in Passer domesticus and environmental remote sensing for inferring the quality of wild, rural, and urban areas

Chromatin Dispersion Test to Asses DNA Damage in Cervical Epithelial Cells

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