Detection of DNA damage in individual cells from marine organisms using the single cell gel assay

Nacci, Diane; Cayula, Stephanie; Jackim, Eugene

doi:10.1016/0166-445x(96)00016-1

Cited by 149 publications

(71 citation statements)

References 29 publications

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“…This assay has been applied in aquatic environments both on vertebrate and invertebrate organisms [43][44][45][46]. In particular, in vivo data on fish were obtained with redbreast sunfish (Lepomis auritus), hard head cat fish (Anus felis), bullhead (Ameirurus nebulosus) and carp (C. carpio) [45,47].…”

Section: Introductionmentioning

confidence: 99%

Comet assay and micronucleus test in circulating erythrocytes of Cyprinus carpio specimens exposed in situ to lake waters treated with disinfectants for potabilization

Buschini

Martino

Gustavino

et al. 2004

Mutation Research/Genetic Toxicology and Environmental Mutagene

114

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The detection of a possible genotoxic effect of surface water treated with disinfectants for potabilization is the aim of the present work. The Comet assay and the micronucleus test were applied in circulating erythrocytes of Cyprinus carpio. Young specimens (20-30 g) were exposed in experimental basins, built within the potabilization plant of Castiglione del Lago (Perugia, Italy). In this plant the water of the Trasimeno Lake is treated and disinfected for potabilization before it is distributed to the people in the net of drinkable water. A continuous flow of water at a constant rate was supplied to basins; the water was continuously treated at a constant concentration with one of the three tested disinfectants (sodium hypochlorite, peracetic acid and chloride dioxide), one control basin being supplied with untreated water. Three sampling campaigns were performed:

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Section: Introductionmentioning

confidence: 99%

Comet assay and micronucleus test in circulating erythrocytes of Cyprinus carpio specimens exposed in situ to lake waters treated with disinfectants for potabilization

Buschini

Martino

Gustavino

et al. 2004

Mutation Research/Genetic Toxicology and Environmental Mutagene

114

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“…They have also served as aquatic pollution indicators. In addition, they may be a source or exposure for local consumers (Nacci et al, 1996;Rinderhagen et al, 2000;Rigonato et al, 2005;Külköylüolu 2004;Regoli et al, 2006). Otherwise, spatial distribution of pollutants on sediments and biota in aquatic ecosystems have been related to a great variety of biological answers in populations and fish/invertebrates communities, with the purpose to determine a possible relation between pollutants in the environment and health in the organisms (Adams et al, 1999).…”

Section: Fish and Invertebratesmentioning

confidence: 99%

Exposure Assessment to Persistent Organic Pollutants in Wildlife: The Case Study of Coatzacoalcos, Veracruz, Mexico

Espinosa-Reyes¹,

González-Mille²,

Ilizaliturri-Hernández³

et al. 2012

Organic Pollutants Ten Years After the Stockholm Convention - Environmental and Analytical Update

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“…One of the most used methods is the alkaline comet assay that was developed by Singh et al (1988), which can detect breaks in the DNA strands, alkali-sensitive sites, and incomplete repair sites (Nacci et al, 1996;Belpaeme et al, 1998). The assay (Koppen and Verschaeve, 1996) and its effectiveness were shown by using the nuclei of Tradescantia (Alvarez-Moya et al, 2001); additionally, the assay could be used to examine the genotoxic activity of several natural products.…”

Section: Introductionmentioning

confidence: 99%

Assessing the genotoxicities of sparteine and compounds isolated from Lupinus mexicanus and L. montanus seeds by using comet assay

Silva¹,

Alvarez²,

Garcia³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The genus Lupinus is widely distributed. Its seeds are used for animal and human food, and Lupinus possesses pharmacological potential because of its high content of quinolizidine alkaloids and flavonoids; however, there is little available information about its genotoxicity. We used the comet assay and staminal nuclei of Tradescantia (clone 4430) to evaluate the in vitro genotoxicity of 4 concentrations (0.01, 0.1, 0.5, and 1.0 mM) of alkaloid extracts of Lupinus mexicanus and Lupinus montanus, flavonoids of L. mexicanus, and commercial sparteine; nitrosodiethylamine was used as a positive control and untreated nuclei were used as a negative control. All concentrations of L. mexicanus and L. montanus showed significant genotoxic activity (P ≤ 0.05). A similar behavior was observed for flavonoid extracts of L. montanus except the 1.0 mM concentration. Sparteine showed genotoxic activity only at 0.5 mM. The order of Genotoxicities of Lupinus extracts genotoxicity of the compounds studied was as follows: L. mexicanus > L. montanus > flavonoids of L. montanus > sparteine. There is evident genotoxic activity in the compounds that were studied, particularly at lower concentrations (0.01 and 0.1 mM). Given the limited information about the genotoxicity of the compounds of L. mexicanus and L. montanus, further studies are necessary.

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Detection of DNA damage in individual cells from marine organisms using the single cell gel assay

Cited by 149 publications

References 29 publications

Comet assay and micronucleus test in circulating erythrocytes of Cyprinus carpio specimens exposed in situ to lake waters treated with disinfectants for potabilization

Comet assay and micronucleus test in circulating erythrocytes of Cyprinus carpio specimens exposed in situ to lake waters treated with disinfectants for potabilization

Exposure Assessment to Persistent Organic Pollutants in Wildlife: The Case Study of Coatzacoalcos, Veracruz, Mexico

Assessing the genotoxicities of sparteine and compounds isolated from Lupinus mexicanus and L. montanus seeds by using comet assay

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