Detection of DNA using bioactive paper strips

Ali, M. Monsur; Aguirre, Sergio D.; Xu, Yaqin; Filipe, Carlos D. M.; Pelton, Robert; Li, Yingfu

doi:10.1039/b911559e

Cited by 111 publications

(81 citation statements)

References 34 publications

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“…A few other assay formats involving bioactive paper and related flow technology have also been reported. [148][149][150] Due to its simplicity (in fabrication and for use), lateral flow technology is one of the most successful platforms for developing bioanalytical assays. We speculate that this might be one of the first platforms for aptamer-based diagnosis to be commercialized.…”

Section: Electrochemistry Sensorsmentioning

confidence: 99%

Aptamer-based biosensors for biomedical diagnostics

Zhou

Huang

Ding

et al. 2014

Analyst

475

290

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Aptamers are single-stranded nucleic acids that selectively bind to target molecules. Most aptamers are obtained through a combinatorial biology technique called SELEX. Since aptamers can be isolated to bind to almost any molecule of choice, can be readily modified at arbitrary positions and they possess predictable secondary structures, this platform technology shows great promise in biosensor development. Over the past two decades, more than one thousand papers have been published on aptamer-based biosensors. Given this progress, the application of aptamer technology in biomedical diagnosis is still in a quite preliminary stage. Most previous work involves only a few model aptamers to demonstrate the sensing concept with limited biomedical impact. This Critical Review aims to summarize progresses that might enable practical applications of aptamer for biological samples. First, general sensing strategies based on the unique properties of aptamers are summarized. Each strategy can be coupled to various signaling methods. Among these, a few detection methods including fluorescence lifetime, flow cytometry, upconverting nanoparticles, nanoflare technology, magnetic resonance imaging, electronic aptamer-based sensors, and lateral flow devices have been discussed in more detail since they are more likely to work in a complex sample matrix. The current limitations of this field include the lack of high quality aptamers for clinically important targets. In addition, the aptamer technology has to be extensively tested in a clinical sample matrix to establish reliability and accuracy. Future directions are also speculated to overcome these challenges.3

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Section: Electrochemistry Sensorsmentioning

confidence: 99%

Aptamer-based biosensors for biomedical diagnostics

Zhou

Huang

Ding

et al. 2014

Analyst

475

290

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“…While glass and gold surfaces are among the most frequently used ones for DNA immobilization, other surfaces such as paper (17)(18)(19), diamond (20), silicon (21), and lipid bilayer (22) have also been tested. We seek to engineer surfaces that allow convenient operation like in homogeneous assays, can be observed by the naked eye with a low optical background, while still maintain the signal amplification capability of immobilized sensors.…”

Section: Introductionmentioning

confidence: 99%

DNA-Functionalized Monolithic Hydrogels and Gold Nanoparticles for Colorimetric DNA Detection

Baeissa

Dave

Smith

et al. 2010

ACS Appl. Mater. Interfaces

127

115

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Highly sensitive and selective DNA detection plays a central role in many fields of research and various assay platforms have been developed. Compared to homogeneous DNA detection, surface immobilized probes allow washing steps and signal amplification to give higher sensitivity. Previously research was focused on developing glass or gold based surfaces for DNA immobilization, we herein report hydrogel immobilized DNA. Specifically, acrydite-modified DNA was covalently functionalized to the polyacrylamide hydrogel during gel formation. There are several advantages of these DNAfunctionalized monolithic hydrogels. First, they can be easily handled in a way similar to that in homogeneous assays. Second, they have a low optical background where in combination with DNAfunctionalized gold nanoparticles, even ~0.1 nM target DNA can be visually detected. By using the attached gold nanoparticles to catalyze the reduction of Ag + , as low as 1 pM target DNA can be detected. The gels can be regenerated by a simple thermal treatment and the regenerated gels perform similarly compared to freshly prepared ones. The amount of gold nanoparticles adsorbed through DNA hybridization decreases with increasing gel percentage. Other parameters including DNA concentration, DNA sequence, ionic strength of the solution and temperature have also been systematically characterized in this study.

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“…Cellulose, the most abundant biopolymer, is a superior candidate for fabrication of bioactive papers because of its biocompatibility and the accumulated knowledge about the material. Cellulose has been used as an ideal support material for bioactive systems, which utilize the pores of the cellulosic matrix to entrap biomolecules [139][140][141][142][143]. The internal surface of pores can be chemically modified, and the functionalized surfaces offer a high selectivity and reactivity for molecular recognition of pathogens.…”

Section: Cellulosic Honeycomb Filmsmentioning

confidence: 99%

Post-polymerization Modification of Surface-Bound Polymers

Théato

2015

Controlled Radical Polymerization at and From Solid Surfaces

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Surfaces that have been intricately functionalized with reactive polymers have attracted scientific attention recently because of their potential use in a broad range of applications. Polymers containing chemically reactive functional groups can be utilized for subsequent modification of various surfaces. Reactive polymeric surfaces can be produced by surface-initiated polymerization, such as atom transfer radical polymerization, nitroxide-mediated polymerization, and ring-opening metathesis polymerization. Such surfaces can subsequently undergo postpolymerization modification to alter their physicochemical properties. Postpolymerization modification has a number of advantages, including the fact that diverse polymer structures are rapidly accessible without individual synthesis; polymerization of new functional monomers can produce a variety of surfaces and interfaces; and other materials can be easily modified, which would be difficult using conventional direct polymerization. In addition, the libraries of chemical reactions and materials that can be used in post-polymerization modifications are abundant. Therefore, post-polymerization modification opens up new platforms for the facile and versatile modification of various surfaces. This chapter focuses on a discussion of post-polymerization modification of various surface-bound polymers, from planar surfaces to three-dimensional objects, and on the extended applications of the reactive surfaces.

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Detection of DNA using bioactive paper strips

Cited by 111 publications

References 34 publications

Aptamer-based biosensors for biomedical diagnostics

Aptamer-based biosensors for biomedical diagnostics

DNA-Functionalized Monolithic Hydrogels and Gold Nanoparticles for Colorimetric DNA Detection

Post-polymerization Modification of Surface-Bound Polymers

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