Detection of Early Prostate Cancer Using a Hepsin-Targeted Imaging Agent

Kelly, Kimberly A.; Setlur, Sunita R.; Ross, Robert W.; Anbazhagan, R.; Waterman, Peter; Rubin, Mark A.; Weissleder, Ralph

doi:10.1158/0008-5472.can-07-1349

Cited by 105 publications

(80 citation statements)

References 25 publications

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“…The NEB linear 7-mer phage library was selected against PC3 prostate cancer cells that were transfected to express hepsin on their surface (Kelly et al 2008b). Negative selections against untransfected PC3 cells were included to remove library members that bound to unwanted cell surface molecules for improved target specificity.…”

Section: Molecular Imaging Agents Obtained Using Cell Lines Expressinmentioning

confidence: 99%

Phage display and molecular imaging: expanding fields of vision in living subjects

Cochran

2010

Biotechnology and Genetic Engineering Reviews

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In vivo molecular imaging enables non-invasive visualization of biological processes within living subjects, and holds great promise for diagnosis and monitoring of disease. The ability to create new agents that bind to molecular targets and deliver imaging probes to desired locations in the body is critically important to further advance this field. To address this need, phage display, an established technology for the discovery and development of novel binding agents, is increasingly becoming a key component of many molecular imaging research programs. This review discusses the expanding role played by phage display in the field of molecular imaging with a focus on in vivo applications. Furthermore, new methodological advances in phage display that can be directly applied to the discovery and development of molecular imaging agents are described. Various phage library selection strategies are summarized and compared, including selections against purified target, intact cells, and ex vivo tissue, plus in vivo homing strategies. An outline of the process for converting polypeptides obtained from phage display library selections into successful in vivo imaging agents is provided, including strategies to optimize in vivo performance. Additionally, the use To whom correspondence may be addressed (cochran1@stanford.edu) Abbreviations: scFv, single chain variable fragment; Fab, fragment antigen binding; ELISA, Enzyme-Linked Immunosorbent Assay; NEB, New England Biolabs; PET, positron emission tomography; SPECT, single photon emission computed tomography; NIR, near infrared; PEG, polyethylene glycol; SPARC, secreted protein acidic and rich in cysteine; VCAM-1, vascular cell adhesion molecule; MRI, magnetic resonance imaging; DOTA, 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid; IC 50 , half maximal inhibitory concentration; CT, computed tomography; K D , equilibrium dissociation constant; EGFR, epidermal growth factor receptor; EGFR-ECD, EGFR extracellular domain; Aβ 42 , amyloid-beta; MMP, matrix metalloprotease; uPAR, urokinase-type plasminogen activator receptor; VEGF, vascular endothelial growth factor; IL-11, Interleukin-11; IL-11αR, Interleukin-11 α-receptor. 58F.V. CoChran and J.r. CoChran of phage particles as imaging agents is also described. In the latter part of the review, a survey of phage-derived in vivo imaging agents is presented, and important recent examples are highlighted. Other imaging applications are also discussed, such as the development of peptide tags for site-specific protein labeling and the use of phage as delivery agents for reporter genes. The review concludes with a discussion of how phage display technology will continue to impact both basic science and clinical applications in the field of molecular imaging.

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Section: Molecular Imaging Agents Obtained Using Cell Lines Expressinmentioning

confidence: 99%

Phage display and molecular imaging: expanding fields of vision in living subjects

Cochran

2010

Biotechnology and Genetic Engineering Reviews

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“…Recently, several types of fluorescent nanoparticles have been employed for the detection of biomarkers (Goutayer et al, 2010;Kelly et al, 2008;Tsai et al, 2011;Wu et al, 2008). For this purpose conjugated polyelectrolytes (CPEs) have been employed since their photophysical properties are known to vary upon interaction with proteins (Li and Liu, 2010;Song et al, 2011).…”

Section: Synthesis Of Polymeric Nanoparticlesmentioning

confidence: 99%

Polymeric nanoparticles for optical sensing

Canfarotta

Whitcombe

Piletsky

2013

Biotechnology Advances

121

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Nanotechnology is a powerful tool for use in diagnostic applications. For these purposes a variety of functional nanoparticles containing fluorescent labels, gold and quantum dots at their cores have been produced, with the aim of enhanced sensitivity and multiplexing capabilities. This work will review progress in the application of polymeric nanoparticles in optical diagnostics, both for in vitro and in vivo detection, together with a discussion of their biodistribution and biocompatibility.

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“…Antibodies (Serada et al 2007) and aptamers (Wang et al 2008) targeting the prostate-specific membrane antigen expressed on prostate cancer cells have been conjugated to SPIO, facilitating selective binding and uptake. Selective imaging of prostate cancer cells in vivo was also recently achieved by the conjugation of SPIO to peptides targeting hepsin, a prostate cancer biomarker (Kelly et al 2008). …”

Section: Introductionmentioning

confidence: 99%

Synthesis of bombesin-functionalized iron oxide nanoparticles and their specific uptake in prostate cancer cells

et al. 2009

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The imaging of molecular markers associated with disease offers the possibility for earlier detection and improved treatment monitoring. Receptors for gastrin-releasing peptide are overexpressed on prostate cancer cells offering a promising imaging target, and analogs of bombesin, an amphibian tetradecapeptide have been previously demonstrated to target these receptors. Therefore, the pan-bombesin analog [β-Ala11, Phe13, Nle14]bombesin-(7-14) was conjugated through a linker to dye-functionalized superparamagnetic iron oxide nanoparticles for the development of a new potential magnetic resonance imaging probe. The peptide was conjugated via click chemistry, demonstrating a complementary alternative methodology to CIHR Author ManuscriptCIHR Author Manuscript CIHR Author Manuscript conventional peptide-nanoparticle conjugation strategies. The peptide-functionalized nanoparticles were then demonstrated to be selectively taken up by PC-3 prostate cancer cells relative to unfunctionalized nanoparticles and this uptake was inhibited by the presence of free peptide, confirming the specificity of the interaction. This study suggests that these nanoparticles have the potential to serve as magnetic resonance imaging probes for the detection of prostate cancer.

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Detection of Early Prostate Cancer Using a Hepsin-Targeted Imaging Agent

Cited by 105 publications

References 25 publications

Phage display and molecular imaging: expanding fields of vision in living subjects

Phage display and molecular imaging: expanding fields of vision in living subjects

Polymeric nanoparticles for optical sensing

Synthesis of bombesin-functionalized iron oxide nanoparticles and their specific uptake in prostate cancer cells

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