Detection of elevated levels of 2-5A synthetase in serum from children with various infectious diseases

Sugino, Hirotoshi; Mitani, Ikuo; Koike, Makoto; Kodama, Takashi; Sokawa, Junko; Sawai, Hiroaki; Ishibashi, K; Itoh, Masao; Watanabe, Shōichi; Sokawa, Yoshihiro

doi:10.1128/jcm.24.3.478-481.1986

Cited by 24 publications

(3 citation statements)

References 15 publications

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“…One such intermediate is 2′‐5′ oligoadenylate synthetase (2‐5AS), an intracellular enzyme induced by all three types of IFNs – alfa, beta and gamma. 2‐5AS catalyzes the formation of 2′‐5′ oligoadenylate (2‐5A) from adenosine triphosphate in the presence of double‐stranded RNA 9–14 . 2‐5AS is thought to play an important role in the antiviral action of IFN through its control of the ribonuclease system, which is activated by 2‐5AS.…”

Section: Introductionmentioning

confidence: 99%

In vivo interferon system assessed by 2′‐5′ oligoadenylate synthetase activity in chronic hepatitis C virus patients treated with pegylated interferon and ribavirin

Shindo

Hamada

Morikawa

et al. 2008

Hepatology Research

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Section: Introductionmentioning

confidence: 99%

In vivo interferon system assessed by 2′‐5′ oligoadenylate synthetase activity in chronic hepatitis C virus patients treated with pegylated interferon and ribavirin

Shindo

Hamada

Morikawa

et al. 2008

Hepatology Research

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“…2'.5'-Oligoadenylate synthetase (2)(3)(4)(5), an enzyme specifically induced by IFN, is thought to reflect the in t,iL,o state of the IFN system (15-17). 2-5 AS activity in peripheral blood mononuclear cells (PBMC) and serum can be detected even in patients whose serum IFN level is undetectable (12, 14), and its measurement is easy and reproducible (18,19). It has been reported that patients with CHB have normal levels of 2-5 AS activity in PBMC and serum (15,19); however, 2-5 AS activity during acute exacerbation has not been shown.…”

mentioning

confidence: 99%

Elevated levels of 2′,5′-oligoadenylate synthetase activity in peripheral blood mononuclear cells and serum during acute exacerbation of chronic hepatitis B

Shindo¹,

Okuno²,

Arai³

et al. 1989

Hepatology

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We measured 2',5'-oligoadenylate synthetase activity in peripheral blood mononuclear cells and serum of 14 HBsAg- and HBeAg-positive patients with chronic hepatitis B with or without acute exacerbation. Elevated levels of 2',5'-oligoadenylate synthetase in peripheral blood mononuclear cells and serum were found in seven chronic hepatitis B patients with acute exacerbation, whereas in the remaining seven chronic hepatitis B patients without acute exacerbation, both levels were similar to those of normal subjects despite active hepatitis B virus multiplication. 2',5'-Oligoadenylate synthetase levels in peripheral blood mononuclear cells and serum, which were not statistically different from those of normal subjects prior to acute exacerbation, increased during acute exacerbation from 3- to 23-fold over initial levels following elevations in ALT activity. 2',5'-Oligoadenylate synthetase levels fluctuated over a normal range while ALT levels were elevated, and they returned to a baseline with ALT normalization. This suggests that the in vivo interferon system may be activated during acute exacerbation, and that this activation may not be a result of hepatitis B virus multiplication alone, but also of a host-immune response to hepatitis B virus multiplication. Three patients were treated with interferon during acute exacerbation. All three had elevated levels of 2',5'-oligoadenylate synthetase in peripheral blood mononuclear cells and serum just before treatment. 2',5'-Oligoadenylate synthetase levels increased only 1.1- to 2.2-fold over initial levels during treatment, with none of the patients clearing HBeAg during and after treatment.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…It has been shown that 2',5'-oligo(A) synthetase activity is increased in PBML during virus infection (2,4,7,20,22,37,38) or treatment with IFN (20,23,26) and that the increased activity persists for at least 24 h after administration of IFN (26). Activity of 2',5'-oligo(A) synthetase has therefore been measured to determine whether humans and other animals have been infected with virus or are responding to IFN (4,7,12,23,25,26,37,40,44). Viral diseases in cattle cause major economic losses throughout the world.…”

mentioning

confidence: 99%

Induction and measurement of 2',5'-oligoadenylate synthetase in Madin-Darby bovine kidney cells and in cattle

Short

1987

J Clin Microbiol

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2',5'-Oligoadenylate [2',5'-oligo(A)] was separated from 14C-labeled nucleosides produced in the 2',5'-oligo(A) synthetase assay by using 100-microliters columns of Dowex 1. No detectable nucleoside remained on the column after elution with 20 column volumes of water, whereas less than 1% of oligonucleotides were eluted from the column. At least 99% of oligonucleotides were eluted from the column with 1 M NaCl, pH 2. The major product had properties consistent with 2',5'-oligo(A). Exposure to alpha-1 bovine interferon (IFN) caused an increase in cellular 2',5'-oligo(A) synthetase activity which was proportional to the concentration of IFN in the medium up to 10(4) U of IFN per ml and then leveled off at about 15 X control activity. Under the assay conditions used, 2',5'-oligo(A) synthetase activity was directly proportional to the amount of cell extract over a 10-fold range. Cattle inoculated with IBR/BVD/PI-3 modified live virus vaccine showed an increase in 2',5'-oligo(A) synthetase activity in peripheral blood mononuclear leukocytes which persisted for at least 3 days postvaccination. Intramuscular injection of cattle with IFN caused a similar increase in 2',5'-oligo(A) synthetase activity. Changes in 2',5'-oligo(A) synthetase activity should be of value in (i) assessing the response of cattle to experimental viral infections or inoculations with viral vaccines or IFN or (ii) indicating a possible viral etiology in disease.

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Detection of elevated levels of 2-5A synthetase in serum from children with various infectious diseases

Abstract: By a sensitive radioimmunoassay method, (2'-5')oligoadenylate synthetase was detected in serum from patients with viral, bacterial, or mycoplasmal infections at elevated levels compared with enzyme levels in serum from healthy individuals and patients suffering from noninfectious diseases.

Cited by 24 publications

References 15 publications

In vivo interferon system assessed by 2′‐5′ oligoadenylate synthetase activity in chronic hepatitis C virus patients treated with pegylated interferon and ribavirin

In vivo interferon system assessed by 2′‐5′ oligoadenylate synthetase activity in chronic hepatitis C virus patients treated with pegylated interferon and ribavirin

Elevated levels of 2′,5′-oligoadenylate synthetase activity in peripheral blood mononuclear cells and serum during acute exacerbation of chronic hepatitis B

Induction and measurement of 2',5'-oligoadenylate synthetase in Madin-Darby bovine kidney cells and in cattle

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