Detection of environmental pollutants using optical biosensor with immobilized algae cells

Frense, Dieter; Müller, Adrian; Beckmann, D.

doi:10.1016/s0925-4005(98)00203-2

Cited by 101 publications

(55 citation statements)

References 5 publications

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“…At high flow rate, the overall response time tends to approach the effective biosensor response time, which is about 10 min. This agrees with data reported by Frense et al (1998). I II II I.…”

Section: Resultssupporting

confidence: 93%

“…Excitation maximum corresponds to the Soret band maximum absorption of chlorophyll b (Hipkins and Baker, 1986) present in large amounts in green algae. Emission maximum corresponds to the main emission peak (fluorescent PSII band) for C. vulgaris (Govindjee and Satoh, 1986) and Scenedesmus subspicatus (Frense et al, 1998). This biosensor has been designed from practical considerations.…”

Section: Resultsmentioning

confidence: 99%

“…Some biosensing systems for herbicides detection use isolated chloroplasts or intact cells of algae to measure changes in chlorophyll fluorescence (Samson and Popovic, 1988;Arsalane et al, 1993;Conrad et al, 1993;Yoneyama et al, 1993;Merz et al, 1996;El Jay et al, 1997 andVan der Heever andGrobbelaar, 1998). These reagentless biosensors are compatible for in-field use or on line monitoring (Weston and Robinson, 1991;Frense et al, 1998 andNaessens et al, 2000). However, so far the limit of detection for atrazine reported with biosensors varied from 1 to 103 μg l −1 depending on the bioreceptor used (Giardi et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Optical whole-cell biosensor using Chlorella vulgaris designed for monitoring herbicides

Védrine

Leclerc²,

Durrieu

et al. 2003

Biosensors and Bioelectronics

155

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. Optical whole-cell biosensor using Chlorella vulgaris designed for monitoring herbicides. Biosensors and Bioelectronics, Elsevier, 2003, 18 (4), pp.547-463. <10.1016/S0956-5663(02)00157-4>. & Bioelectronics, 2003& Bioelectronics, , 18(4), 457-63, doi:10.1016 Biosensors AbstractAn optical biosensor was designed for determination of herbicides as aquatic contaminants. Detection was obtained with immobilised Chlorella vulgaris microalgae entrapped on a quartz microfibre filter and placed in a five-membrane-home-made-flow cell. The algal chlorophyll fluorescence modified by the presence of herbicides was collected at the tip of an optical fibre bundle and sent to a fluorimeter. A continuous culture was set up to produce algal cells in reproducible conditions for measurement optimisation. Effects of flow rate, algal density, temperature, and pH on the biosensor response to atrazine were studied. Reversibility and detection limits were determined for DNOC and atrazine, simazine, isoproturon, diuron. Detection of photosystem II (PSII) herbicides was achieved at sub-ppb concentration level. K Ke ey yw wo or rd ds s: :

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Optical whole-cell biosensor using Chlorella vulgaris designed for monitoring herbicides

Védrine

Leclerc²,

Durrieu

et al. 2003

Biosensors and Bioelectronics

155

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“…For the microalgae-based detection of toxicants, immobilization in connection with entrapment in a cross-linking matrix is required when incorporating microalgae in a biosensor, in particular when algae will be exposed to a flowing solution. However, cell entrapment was shown to decrease the sensitivity of microalgal biosensors by an order of magnitude compared with nonentrapped cells [14,15]. Limited previous studies on algal sensor chips demonstrated that the photosynthesis inhibition was the physiological end point most often chosen as the response signal to toxicants [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Microalgal motility measurement microfluidic chip for toxicity assessment of heavy metals

2012

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A polydimethylsiloxane microfluidic chip has been developed for the estimation of toxic heavy metals based on measurement of mobility of marine microalgae. The chip is mainly composed of an upstream concentration gradient generator and a downstream perfusion-based chemotatic module. The processes of toxic liquid dilution and diffusion, microalgal culturing, cell stimulation, and online screening can be integrated in this chip, which makes it an attractive approach to simplify toxicity testing procedures. The microalgal motility was adopted as a microfluidic bioassay signal and was evaluated as the percentage of motile cells, curvilinear velocity, average path velocity, and straight line velocity. Two mobile marine microalgae, Platymonas subcordiformis and Platymonas helgolandica var. tsingtaoensis, were confined in the chemotatic module and stimulated by the eight concentration gradients of Cu and Cd generated by the concentration gradient generator. In all cases, a toxic response was detected (i.e., a dose-related inhibition of motility was observed). Only 1.5 h was needed to predict EC 50 values. Thus, the microfluidic chip developed was proved to be useful as a simple and rapid approach in heavy metal detection and might be expanded as a conventional test method in environmental toxicity assessment.

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“…Taking advantage of this feature, photosystem II (PSII)-based biosensors are reported to be able to detect herbicides in the environment (Giardi et al, 2001). For example, an optical fiber based biosensor was developed for atrazine and endrine monitoring in water using Scenedesmus subspicatus cells, immobilized on filter paper and covered with a thin alginate layer hardened with calcium chloride (Frense et al, 1998). Chlorella vulgaris was immobilized at the tip of an optical fiber bundle placed inside a homemade microcell (Naessens et al, 2000), or in a rotating support holding up to five different membranes to increase the number of assays (Védrine et al, 2003), and used for the detection of herbicides affecting photosystem II (PSII) such as triazines (atrazine, simazine) or phenylureas (diuron, isoproturon) herbicides at sub-µgL -1 concentration level.…”

Section: Microalgal Whole Cell Biosensorsmentioning

confidence: 99%

Highly Specific Biosensors to Herbicides, Based on Sensitive- and Resistant-Mutants of Microalgae

Hernández-Allica¹,

Carrera-Martínez²,

López‐Rodas³

et al. 2011

Herbicides and Environment

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Detection of environmental pollutants using optical biosensor with immobilized algae cells

Cited by 101 publications

References 5 publications

Optical whole-cell biosensor using Chlorella vulgaris designed for monitoring herbicides

Optical whole-cell biosensor using Chlorella vulgaris designed for monitoring herbicides

Microalgal motility measurement microfluidic chip for toxicity assessment of heavy metals

Highly Specific Biosensors to Herbicides, Based on Sensitive- and Resistant-Mutants of Microalgae

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