Detection of Epstein Barr virus in formalin-fixed paraffin tissues by fluorescent direct in situ PCR

Marziliano, Nicola; Arbustini, Eloisa; Gasperis, Marco Rossi de; Crovella, Sérgio

doi:10.4081/958

Cited by 4 publications

(2 citation statements)

References 10 publications

(6 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this case, it was stated that the viral presence in spermatozoa pellet samples might be due to residual NSC or alternatively, to false positives due to non-specific hybridization of in situ PCR (Persico et al, 2006). Both explanations seem unlikely, as unequivocal sperm identification is guaranteed by its specific morphological features, and in situ PCR, when correctly performed along with positive and negative controls, is considered a powerful, highly reliable technique for the detection of viral DNA in human tissue sections and cells (Marziliano et al, 2005;Trincado et al, 2005;Comar et al, 2006;Nuovo, 2006;Tanji et al, 2006). HIV-1 DNA was revealed in 10 and 5 out of 12 subjects by in situ PCR hybridization and by nested PCR analysis, respectively.…”

Section: Discussionmentioning

confidence: 99%

HIV-1 viral DNA is present in ejaculated abnormal spermatozoa of seropositive subjects

et al. 2007

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

HIV-1 viral DNA is present in ejaculated abnormal spermatozoa of seropositive subjects

et al. 2007

View full text Add to dashboard Cite

show abstract

“…For qRT-PCR [ 29 ], the total RNA of clinical or cell samples was reverse-transcribed with EasyScript cDNA Synthesis SuperMix, and qRT-PCR was performed with SYBR Premix Ex Taq™ II (TaKaRa, Japan), according to the manufacturer's protocol. Primer sequences are presented in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

The Long Noncoding RNA Cytoskeleton Regulator RNA (CYTOR)/miRNA-24-3p Axis Facilitates Nasopharyngeal Carcinoma Progression by Modulating GAD1 Expression

Peng

et al. 2023

Journal of Oncology

View full text Add to dashboard Cite

Nasopharyngeal carcinoma (NPC) is a head and neck epithelial carcinoma that is unusually prevalent in Southeast Asia. Noncoding RNAs, including lncRNA and miRNA, and their target genes are considered vital regulators of tumorigenesis and the progression of NPC. However, the detailed underlying mechanisms of GAD1 involved in the regulation of NPC need to be further elucidated. In the present study, we identified that GAD1 was significantly upregulated in NPC tissues. GAD1 overexpression is promoted, while genetic knockdown of GAD1 suppresses proliferation, colony formation, migration, and invasion of NPC cells. Bioinformatics analysis and a luciferase reporter assay demonstrated that GAD1 is a direct target gene of miR-24-3p. In NPC tissues, miR-24-3p was downregulated and the lncRNA CYTOR was upregulated. CYTOR was sponged to suppress the function of miR-24-3p. CYTOR regulates GAD1 expression via modulating miR-24-3p. The CYTOR/miR-24-3p/GAD1 axis is converged to modulate the growth, migration, and invasion of NPC cells. In conclusion, the study identified a novel axis for the regulation of NPC cell growth, providing new insights into the understanding of NPC.

show abstract

Histochemistry through the years, browsing a long-established journal: novelties in traditional subjects

Pellicciari¹

2010

Eur J Histochem

View full text Add to dashboard Cite

Histochemical journals represent a traditional forum where methodological and technological improvements can be presented and validated in view of their applications to investigate not only cytology and histology in normal and diseased conditions but to test as well hypotheses on more basic issues for life sciences, such as comparative and evolutionary biology. The earliest scientific journals on histochemistry began their publication in the first half of the ‘50s of the last century, and their readership did not probably change over the years; rather, the authors’ interests may have progressively been changing as well as the main topics of their articles. This hypothesis is discussed, based on the subjects of the article published in the first and last ten years in the European Journal of Histochemistry, as an example of old journal which started publication in 1954, being since then the official organ of the Italian Society of Histochemistry. This survey confirmed that histochemistry has provided and still offers unique opportunities for studying the structure, chemical composition and function of cells and tissues in a wide variety of living organisms, especially when the topological distribution of specific molecular components has diagnostic or predictive significance, as it occurs in human and veterinary biology and pathology. Some subjects (e.g. histochemistry applied to muscle cells or to mineralized tissues) have recently become rather popular, whereas a wider application of the histochemical approach may be envisaged for plant cells and tissues.

show abstract

Detection of Epstein Barr virus in formalin-fixed paraffin tissues by fluorescent direct in situ PCR

Cited by 4 publications

References 10 publications

HIV-1 viral DNA is present in ejaculated abnormal spermatozoa of seropositive subjects

HIV-1 viral DNA is present in ejaculated abnormal spermatozoa of seropositive subjects

The Long Noncoding RNA Cytoskeleton Regulator RNA (CYTOR)/miRNA-24-3p Axis Facilitates Nasopharyngeal Carcinoma Progression by Modulating GAD1 Expression

Histochemistry through the years, browsing a long-established journal: novelties in traditional subjects

Contact Info

Product

Resources

About