Detection of ergopeptine alkaloids in endophyte-infected perennial ryegrass by tandem mass spectrometry

Rowan, Daryl D.; Shaw, G. John

doi:10.1080/00480169./1987.35448

Cited by 37 publications

(34 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Variations between site types were not significant and changes over time were not addressed. Further insight into these factors, as well as information on other compounds such as paxilline and ergovaline that are present in A. lolii-infected ryegrass (Weedon & Mantle 1987;Rowan & Shaw 1987), is also required so that the appropriate management, which aims to minimise, if not eliminate, any adverse impact on livestock performance, can be devised.…”

Section: Discussionmentioning

confidence: 99%

Distributions of the fungal endophyteAcremonium lolii,and of the alkaloids lolitrem B and peramine, within perennial ryegrass

Keogh¹,

Tapper²,

Fletcher³

1996

New Zealand Journal of Agricultural Research

View full text Add to dashboard Cite

Ryegrass tillers, from a ryegrass staggers-prone pasture, were dissected into constituent live leaves of different ages. Leaves were further divided into three blade and two sheath portions. Acremonium lolii, lolitrem B, and peramine concentrations were determined for each defined portion. The mean % distribution and concentrations of A. lolii and lolitrem B in the sheath were much higher, and the mean % distribution of peramine in the sheath was much lower, than in the blade portions. Within leaves, both the % distribution and the concentration of A. lolii increased progressively from blade tip to lower sheath; the patterns for % distribution and concentration of lolitrem B were similar to those for A. lolii, except that lower sheath values were less than those of upper sheath portions; the % distribution of peramine was higher in the lower blade portions than elsewhere, and peramine concentrations were higher in portions adjacent to the ligule. Within tillers, A. lolii concentrations were lower in the youngest and oldest leaves; peramine concentrations were lower for older than for younger leaves; and lolitrem B concentrations increased progressively from youngest to oldest leaves.

show abstract

Section: Discussionmentioning

confidence: 99%

Distributions of the fungal endophyteAcremonium lolii,and of the alkaloids lolitrem B and peramine, within perennial ryegrass

Keogh¹,

Tapper²,

Fletcher³

1996

New Zealand Journal of Agricultural Research

View full text Add to dashboard Cite

show abstract

“…In ryegrass, peramine (Rowan & Gaynor 1986), lolitrems (Gallagher et al 1981) and ergot alkaloids (Rowan & Shaw 1987) are produced. All have known effects against insect herbivores ranging from direct toxicity to larvae as for lolitrem B (Prestidge & Gallagher 1985), to adult deterrency as for peramine (Rowan & Gaynor 1986;Rowan et al 1990) and ergovaline (Popay et al 1990).…”

Section: Introductionmentioning

confidence: 99%

Field resistance to Argentine stem weevil(Listronotus bonariensis)in different ryegrass cultivars infected with an endophyte deficient in lolitrem B

Popay

Hume

Mainland

et al. 1995

New Zealand Journal of Agricultural Research

View full text Add to dashboard Cite

A spaced plant trial, a small plot trial, and a field trial evaluated an endophyte (Acremonium spp.) (AR6) which does not produce lolitrem B in the tetraploid cultivars Grasslands Moata (Lolium multiflorum) and Grasslands Greenstone (L. multiflorum × perenne), and in the perennial cultivars (L. perenne) Grasslands Pacific and Grasslands Nui. The endophyte was compared to three lolitrem-producing isolates viz. AR41, Waiau, and the wildtype. In all trials Argentine stem weevil (ASW) (Listronotus bonariensis) adult feeding, oviposition, and larval damage were recorded at regular intervals between October and March. ASW attack was highest on the tetraploid cultivars, Greenstone and Moata. In most instances, all endophytes reduced adult ASW feeding and oviposition in the different cultivars compared with their respective endophyte-free (E-) controls. Feeding and oviposition on endophyte-infected (E+) Moata and Greenstone was in some instances similar to, or higher than, that on E-Nui or Pacific. All endophyte isolates also significantly reduced the incidence and severity of ASW larval damage. ASW damage levels were higher in summer than in spring. Differences as a result of endophyte A95056Received 25 August 1995; accepted 9 November 1995 infection were much greater in summer than in spring. In summer on E+ plants nearly all ASW oviposition and larval damage occurred on Moata and/or Greenstone. There were few significant differences between the different endophyte isolates. In the field trial, the AR6 endophyte gave protection against ASW attack similar to that provided by the wildtype endophyte.

show abstract

“…13,14,18 The second group of toxic alkaloids present in endophyte-infected perennial ryegrass is the ergopeptine alkaloids. 3,17 Ergopeptine alkaloids are also present in tall fescue (Festuca arudinacea) infected with the endophytic fungus Neotiphodium festuca, with ergovaline being the predominant alkaloid. 1,2,15,24 Ergovaline is considered to be a causitive toxin for diseases such as summer slump, a syndrome associated with tall fescue and characterized by poor growth and increased sensitivity to heat stress.…”

mentioning

confidence: 99%

Correlation of Ergovaline and Lolitrem B Levels in Endophyte-Infected Perennial Ryegrass (Lolium Perenne)

Hovermale

Craig

2001

J VET Diagn Invest

View full text Add to dashboard Cite

Abstract. The varieties of perennial ryegrass (Lolium perenne) infected with the endophytic fungus Neotiphodium lolii contain several classes of toxic alkaloids, including ergopeptide alkaloids and lolitrem alkaloids. Lolitrem B, a potent tremorgen, is generally considered to be the predominant alkaloid in endophyte-infected perennial ryegrass. Ergovaline, a vasoconstrictor normally associated with endophyte-infected tall fescue (Festuca arudinacea), is also present in endophyte infected perennial ryegrass. Clinical signs of animals ingesting endophyte-infected perennial ryegrass are consistent with the presence of lolitrem B. However, clinical signs normally associated with ergovaline poisoning are not usually observed in animals ingesting endophyte-infected perennial ryegrass. A survey was conducted to quantitate both lolitrem B and ergovaline in 459 perennial ryegrass straw samples received at the Oregon State University College of Veterinary Medicine. Samples were analyzed for each alkaloid using separate high-performance liquid chromatography analyses. A strong positive correlation between the 2 alkaloids (r 2 ϭ 0.7335) was observed, especially in the samples containing Ͻ3,000 ppb (ng/g) lolitrem B. The threshold levels above which clinical signs typically occur are 2,000 ppb lolitrem B and 300-400 ppb ergovaline. All of the samples analyzed contained Ͻ425 ppb ergovaline.

show abstract

Detection of ergopeptine alkaloids in endophyte-infected perennial ryegrass by tandem mass spectrometry

Cited by 37 publications

References 6 publications

Distributions of the fungal endophyteAcremonium lolii,and of the alkaloids lolitrem B and peramine, within perennial ryegrass

Distributions of the fungal endophyteAcremonium lolii,and of the alkaloids lolitrem B and peramine, within perennial ryegrass

Field resistance to Argentine stem weevil(Listronotus bonariensis)in different ryegrass cultivars infected with an endophyte deficient in lolitrem B

Correlation of Ergovaline and Lolitrem B Levels in Endophyte-Infected Perennial Ryegrass (Lolium Perenne)

Contact Info

Product

Resources

About