Detection of Escherichia coli adhesins with DNA probes

Lanser, J.B.K.; Anargyros, Peter

doi:10.1128/jcm.22.3.425-427.1985

Cited by 12 publications

(1 citation statement)

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“…ETEC identification by DNA hybridization was compared with immunofluorescence for F4, F5, F6, and F41 fimbrial antigens. The correlation between the results of hybridization with F4, F5, F6, and F41 probes and phenotypic testing for fimbrial antigen production confirmed the suitability of the probe for detecting fimbrial genes in porcine ETEC, as reported for some fimbriae (23,30). Tests for the detection of F4 fimbrial antigen and genes showed perfect agreement.…”

Section: Discussionsupporting

confidence: 68%

Detection of genes for fimbrial antigens and enterotoxins associated with Escherichia coli serogroups isolated from pigs with diarrhea

et al. 1991

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pigs with diarrhea were examined for serogroup and fimbrial antigen F4 (K88) production. Four main patterns of isolation of the various serogroups were observed, depending on the ages of the pigs from which isolates were obtained and the production of F4. In pattern I, serogroups 08:K"S16", 09:K35, 09/0101:K30, 09/0101:K103, 09 (group), 020:K101, and 064:K"V142" were predominant in pigs aged 0 to 6 days (41.9% of isolates) and were less frequent in pigs aged 7 to 27 days (24.6% of isolates) but were rarely found in pigs aged 28 to 60 days (4.0% of isolates). In pattern II, the F4-associated serogroups 08:K"4627", 0157:K"V17", 0149:K91, and 0147:K89 were predominant in pigs aged 7 to 27 days (29.8% of isolates) and in pigs aged 28 to 60 days (35.0% of isolates). In pattern III, serogroups 08 (group), 0115:K"V165", and 0147:K89 were rarely isolated from pigs aged 0 to 6 days but were equally distributed in pigs aged 7 to 27 days (10.1% of isolates) and in pigs aged 28 to 60 days (10.9% of isolates). In pattern IV, serogroups 0138:K81, 0139:K82, 0141:K85ac, 045:K"E65", and 026:K60 were most frequently isolated in pigs aged 28 to 60 days (19.3% isolates). Over the period from 1979 to 1989, the proportion of isolates belonging to serogroups of pattern II and the proportion of F4 isolates within the serogroup 0157:K"V17" declined, whereas the proportion of isolates of serogroups 0147:K89, 08:K"S16", and 09:K35 increased. For 228 isolates selected from the most important serogroups, good agreement was observed between the results of gene probes and immunofluorescence for the detection of fimbrial antigens F4 (K88), F5 (K99), F6 (987P), and F41 and between the results of gene probes and biological assays for the detection of heat-labile enterotoxin (LT) and heat-stable enterotoxins a and b (STa and STb). The STa gene was mostly associated with isolates of pattern I serogroups, which had the F5, F6, and F41 genes alone or in various combinations. The LT and/or STb genes, with the F4 gene, mostly were observed in isolates of pattern II serogroups. The STb gene alone was observed mostly in isolates of pattern III serogroups, although isolates were negative for all fimbrial antigen genes. Similarly, isolates of pattern IV serogroups were negative for all fimbrial antigen genes and rarely positive for the enterotoxin genes. However, verotoxin production was associated with isolates of serogroups 0138:K81 and 0139:K82. The most important pathotypes among * Corresponding author.

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Section: Discussionsupporting

confidence: 68%