Use of nucleic acid probes in the diagnosis of diarrheal disorders

Echeverria, Peter; Taylor, David N.; Seriwatana, Jitvimol; Sethabutr, Orntipa; Chatkaeomorakot, Arunsri

doi:10.1007/bf02751228

Cited by 3 publications

(2 citation statements)

References 51 publications

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“…After fixation on Whatman no. 541 filters (11), E. coli colonies were tested for hybridization with the EAF probe (12) and with a probe to identify EIEC (16), as previously described (8). E.…”

Section: Methodsmentioning

confidence: 99%

Importance of salmonellae and Campylobacter jejuni in the etiology of diarrheal disease among children less than 5 years of age in a community in Bangkok, Thailand

et al. 1990

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The etiology of diarrhea in children <5 years of age in a low-income housing project in Bangkok, Thailand, was determined over 1 year. Nontyphoidal salmonella (13%), Campylobacterjejuni (12%), rotavirus (12%), enterotoxigenic Escherichia coli (7%), shigellae (6%), E. coli that hybridized with the enteropathogenic E. coli adherence factor probe (3%), and enteroinvasive E. coli (1%) were identified in 345 episodes of diarrhea in children <5 years of age. Salmonellae were identified in 17% and C. jejuni was identified in 15% of 54 children <6 months of age with diarrhea. Shigellae, enteroinvasive E. coli, enteropathogenic E. coli adherence factor, and enterotoxigenic E. coli were not isolated from children <6 months of age. Since salmonella and C. jejuni were the most common bacterial pathogens identified in children <6 months of age, efforts to prevent transmission of salmonella and campylobacter to young children should be a public health priority in Bangkok.

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Section: Methodsmentioning

confidence: 99%

Importance of salmonellae and Campylobacter jejuni in the etiology of diarrheal disease among children less than 5 years of age in a community in Bangkok, Thailand

et al. 1990

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“…Determinants controlling expression of all three fimbrial types are encoded by non-conjugative plasmids which display heterogeneity in molecular size [1,[3][4][5][6]. Determinants controlling expression of all three fimbrial types are encoded by non-conjugative plasmids which display heterogeneity in molecular size [1,[3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Expression of CS fimbriae in Escherichia coli strains of human and animal origin belonging to O-serovar 6, K-serovar 15 or H-serovar 16

Twohig¹

1988

FEMS Microbiology Letters

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A non‐conjugative CS‐fimbriae‐associated plasmid pCS001 was mobilized into rifampicin‐resistant mutants of strains of Escherichia coli of O‐serovar 6 or K‐serovar 15 or H‐serovar 16. By a variety of serological procedures only the production of CS3 fimbriae was detected in transoconjugants. The finding extend previous observations that only strains of E. coli of serotype O6:K15:H16 or H− and of appropriate biotype are able to express either CS1 or CS2 fimbriae, as even a recipient of serotype O6:K15:H31 possessing a rhamnose‐positive fermentation phenotype did not express either of these two fimbriae. The results indicate that expression of CS1 or CS2 fimbriae probably involves chromosomal determinants only found in strains of serotype O6:K15:H16 or H−.

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The Spectrum of Salmonella Infection

Goldberg

Rubin

1988

Infectious Disease Clinics of North America

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Use of nucleic acid probes in the diagnosis of diarrheal disorders

Cited by 3 publications

References 51 publications

Importance of salmonellae and Campylobacter jejuni in the etiology of diarrheal disease among children less than 5 years of age in a community in Bangkok, Thailand

Importance of salmonellae and Campylobacter jejuni in the etiology of diarrheal disease among children less than 5 years of age in a community in Bangkok, Thailand

Expression of CS fimbriae in Escherichia coli strains of human and animal origin belonging to O-serovar 6, K-serovar 15 or H-serovar 16

The Spectrum of Salmonella Infection

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