Detection of Escherichia coli with a label-free impedimetric biosensor based on lectin functionalized mixed self-assembled monolayer

Yang, Haiying; Hong, Zhou; Hao, Haoyong; Gong, Qiaojuan; Nie, Kai

doi:10.1016/j.snb.2015.08.034

Cited by 79 publications

(61 citation statements)

References 46 publications

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“…For example, lectins have been investigated as biorecognition elements for pathogen detection through their ability to selectively bind glycosylated proteins on the surfaces of viruses and cells (Reina et al 2008). Concanavalin A (ConA) lectin has been extensively investigated for E. coli detection (see Table 1) (Jantra et al 2011;Saucedo et al 2019;Xi et al 2011;Yang et al 2016b). While not yet widely investigated for pathogen detection using electrochemical biosensors, Etayash et al recently showed that oligopeptides also provide attractive biorecognition elements for real-time biosensor-based detection of breast cancer cells (Etayash et al 2015).…”

Section: Carbohydrate-binding Proteinsmentioning

confidence: 99%

Electrochemical biosensors for pathogen detection

Cesewski

Johnson

2020

Biosensors and Bioelectronics

635

358

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A B S T R A C TRecent advances in electrochemical biosensors for pathogen detection are reviewed. Electrochemical biosensors for pathogen detection are broadly reviewed in terms of transduction elements, biorecognition elements, electrochemical techniques, and biosensor performance. Transduction elements are discussed in terms of electrode material and form factor. Biorecognition elements for pathogen detection, including antibodies, aptamers, and imprinted polymers, are discussed in terms of availability, production, and immobilization approach. Emerging areas of electrochemical biosensor design are reviewed, including electrode modification and transducer integration. Measurement formats for pathogen detection are classified in terms of sample preparation and secondary binding steps. Applications of electrochemical biosensors for the detection of pathogens in food and water safety, medical diagnostics, environmental monitoring, and bio-threat applications are highlighted. Future directions and challenges of electrochemical biosensors for pathogen detection are discussed, including wearable and conformal biosensors, detection of plant pathogens, multiplexed detection, reusable biosensors for process monitoring applications, and low-cost, disposable biosensors.

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Section: Carbohydrate-binding Proteinsmentioning

confidence: 99%

Electrochemical biosensors for pathogen detection

Cesewski

Johnson

2020

Biosensors and Bioelectronics

635

358

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“…Although the principles differ among the different methods, most of the sensors under development require a first step aimed at retaining the targeted bacterium with a ligand-coated surface [23] and a second step of identification. Lectins, such as concavalin A, have become one of the selected ligands [24]. The extremely high affinity of BLL2 against a broad spectrum of bacteria could have biotechnological applications, such as trapping bacteria in biosensors, but also in other types of applications involving bacterial capture (i.e., in air or water monitoring).…”

Section: Discussionmentioning

confidence: 99%

High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae)

2017

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Lectins are carbohydrate-interacting proteins that play a pivotal role in multiple physiological and developmental aspects of all organisms. They can specifically interact with different bacterial and viral pathogens through carbohydrate-recognition domains (CRD). In addition, lectins are also of biotechnological interest because of their potential use as biosensors for capturing and identifying bacterial species. In this work, three C-type lectins from the Lepidoptera Spodoptera exigua were produced as recombinant proteins and their bacterial agglutination properties were characterized. The lowest protein concentration producing bacterial agglutination against a panel of different Gram+ and Gram− as well as their carbohydrate binding specificities was determined for the three lectins. One of these lectins, BLL2, was able to agglutinate cells from a broad range of bacterial species at an extremely low concentration, becoming a very interesting protein to be used as a biosensor or for other biotechnological applications involving bacterial capture.

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“…Although the principles are different among the different methods, most of the sensors under development require a first step aimed to retain the targeted bacterium with a ligand-coated surface [23] and a second step of identification. Lectins, such as concavalin A have become one of the selected ligands [24]. The extremely high affinity of BLL2 against a broad spectrum of bacteria could have biotechnological applications, such as to trap bacteria in biosensors, but also in other types of applications involving bacterial capturing (i.e, in air or water monitoring).…”

Section: Discussionmentioning

confidence: 99%

High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from <em>Spodoptera exigua</em> (Lepidoptera: Noctuidae)

Gasmi

Ferré

Herrero

2017

Preprint

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Abstract:Lectins are carbohydrate-interacting proteins playing a pivotal role in multiple physiological and developmental aspects of all organisms. They can specifically interact with different bacterial and viral pathogens through the carbohydrate-recognition domains (CRD). In addition, lectins are also of biotechnological interest because of their potential use as biosensor for capturing and identification of bacterial species. In this work, we have characterized the bacterial agglutination properties of three C-type lectins from the Lepidoptera Spodoptera exigua. One of these lectins, BLL2, was able to agglutinate cells from a broad range of bacterial species at an extremely low concentration, becoming a very interesting protein to be used as biosensor or other biotechnological applications involving bacterial capturing.

show abstract

Detection of Escherichia coli with a label-free impedimetric biosensor based on lectin functionalized mixed self-assembled monolayer

Cited by 79 publications

References 46 publications

Electrochemical biosensors for pathogen detection

Electrochemical biosensors for pathogen detection

High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae)

High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from <em>Spodoptera exigua</em> (Lepidoptera: Noctuidae)

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