Detection of Excretory/Secretory Coproantigens in Experimental Hookworm Infection

Bungiro, Richard D.; Cappello, Michael

doi:10.4269/ajtmh.2005.73.915

Cited by 37 publications

(30 citation statements)

References 33 publications

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“…Sharing similar antigens of over 70% of homology between O. viverrini cathepsin F and other flukes [18] likely explain the weak cross reactivity. Our overall results is in consistent with the studies of coproantigen detection for other helminthic infection including hookworm [37], Fasciola hepatica in cattle and sheep [38,39] as well as detection of circulating antigen of F. gigantica using sandwich ELISA [21,35,39,40]. …”

Section: Discussionsupporting

confidence: 91%

Chicken IgY-based coproantigen capture ELISA for diagnosis of human opisthorchiasis

Teimoori

Arimatsu

Laha

et al. 2017

Parasitology International

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Diagnosis of Opisthorchis viverrini infection by conventional stool examination is increasingly difficult due to the low intensity of the infection after several rounds of control programmes in endemic regions as well as coinfections with intestinal flukes. Therefore sensitive and specific diagnostic test is needed. In this study, a coproantigen sandwich ELISA using recombinant O. viverrini cathepsin F (rOv-CF) was developed. This sandwich ELISA employing chicken IgY raised against rOv-CF in combination with rabbit IgG antibody to the somatic O. viverrini antigens showed a lower detection limit (LLD) of 70 ng native O. viverrini somatic antigens by spiking the parasite antigens into control feces. When applied to the diagnosis, the IgY-based sandwich ELISA exhibited sensitivity and specificity of 93.3% and 76.7%, respectively, in an investigation of 90 human cases positive or negative for opisthorchiasis. The positive predictive value (PPV) and negative predictive value (NPV) for this coproantigen detection were 66.7% and 95.2%, respectively. This IgY-based sandwich ELISA using parasite cathepsin F detection shows a promising immunodiagnostic alternative for human opisthorchiasis in endemic regions.

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Section: Discussionsupporting

confidence: 91%

Chicken IgY-based coproantigen capture ELISA for diagnosis of human opisthorchiasis

Teimoori

Arimatsu

Laha

et al. 2017

Parasitology International

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“…Hookworm eggs can be detected in the feces of hamsters infected with A. ceylanicum as early as day 14 PI, with egg counts increasing sharply over the next several days (26). In the buffer-treated animals, fecal egg counts increased from a mean of 917 Ϯ 84 eggs per g (epg) of feces on day 16 PI to 1,800 Ϯ 167 epg by day 19 PI (Fig.…”

Section: Cry5b Treatment Reduces Fecal Egg Excretion and Intestinal Wormmentioning

confidence: 92%

A purified Bacillus thuringiensis crystal protein with therapeutic activity against the hookworm parasite Ancylostoma ceylanicum

Cappello

Bungiro

Harrison

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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113

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Crystal (Cry) proteins produced by the soil bacterium Bacillus thuringiensis (Bt) are harmless to vertebrates, but they are highly toxic to insects and nematodes. Their value in controlling insects that destroy crops and transmit human diseases is well established. Although it has recently been demonstrated that a few individual Bt Cry proteins, such as Cry5B, are toxic to a wide range of free-living nematodes, the potential activity of purified Cry proteins against parasitic nematodes remains largely unknown. We report here studies aimed at characterizing in vitro and in vivo anthelminthic activities of purified recombinant Cry5B against the hookworm parasite Ancylostoma ceylanicum, a bloodfeeding gastrointestinal nematode for which humans are permissive hosts. By using in vitro larval development assays, Cry5B was found to be highly toxic to early stage hookworm larvae. Exposure of adult A. ceylanicum to Cry5B was also associated with significant toxicity, including a substantial reduction in egg excretion by adult female worms. To demonstrate therapeutic efficacy in vivo, hamsters infected with A. ceylanicum were treated with three daily oral doses of purified Cry5B, the benzimidazole anthelminthic mebendazole, or buffer. Compared with control (buffer-treated) animals, infected hamsters that received Cry5B showed statistically significant improvements in growth and blood hemoglobin levels as well as reduced worm burdens that were comparable to the mebendazole-treated animals. These data demonstrate that Cry5B is highly active in vitro and in vivo against a globally significant nematode parasite and that Cry5B warrants further clinical development for human and veterinary use.anthelminthic ͉ Cry5B ͉ nematode

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“…78,79 Also, the observation that levels of IgG correlate with infection status, at least in children, validates the use of A. ceylanicum as a source of potentially relevant hookworm antigens that can be identified based on reactivity with human sera. Because the laboratory model of A. ceylanicum infection recapitulates the clinical features of human hookworm infection, 19,21,80 specific ES antigens recognized by infected individuals may represent valuable targets for human drug and vaccine development.…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of Hookworm Infection in Kintampo North Municipality, Ghana: Patterns of Malaria Coinfection, Anemia, and Albendazole Treatment Failure

Humphries

Mosites²,

Otchere³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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110

100

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Abstract.A cross-sectional pilot study of hookworm infection was carried out among 292 subjects from 62 households in Kintampo North, Ghana. The overall prevalence of hookworm infection was 45%, peaking in those 11-20 years old (58.5%). In children, risk factors for hookworm infection included coinfection with malaria and increased serum immunoglobulin G reactivity to hookworm secretory antigens. Risk factors for infection in adults included poor nutritional status, not using a latrine, not wearing shoes, and occupation (farming). Although albendazole therapy was associated with an overall egg reduction rate of 82%, 37 subjects (39%) remained infected. Among those who failed therapy, treatment was not associated with a significant reduction in egg excretion, and nearly one-third had higher counts on repeat examination. These data confirm a high prevalence of low-intensity hookworm infection in central Ghana and its association with poor nutritional status. The high rate of albendazole failure raises concern about emerging resistance.

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Detection of Excretory/Secretory Coproantigens in Experimental Hookworm Infection

Cited by 37 publications

References 33 publications

Chicken IgY-based coproantigen capture ELISA for diagnosis of human opisthorchiasis

Chicken IgY-based coproantigen capture ELISA for diagnosis of human opisthorchiasis

A purified Bacillus thuringiensis crystal protein with therapeutic activity against the hookworm parasite Ancylostoma ceylanicum

Epidemiology of Hookworm Infection in Kintampo North Municipality, Ghana: Patterns of Malaria Coinfection, Anemia, and Albendazole Treatment Failure

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