Detection of Feline Leukaemia Virus in Blood and Bone Marrow of Cats with Varying Suspicion of Latent Infection

Herring, ES; Troy, Gregory C.; Te, Toth; Forrester, S. Dru; Weigt, LA; Herring, IP

doi:10.1053/jfms.2001.0132

Cited by 22 publications

(26 citation statements)

References 19 publications

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“…Performing this testing in association with polymerase chain reaction (PCR) not only enables identification of the provirus, independently of the presence of FIV antibodies or of FeLV antigenemia, but also makes it possible to determine the different outcomes from infection by FeLV (Herring et al, 2001;Hofmann-Lehmann et al, 2001;Englert et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Feline immunodeficiency virus and feline leukemia virus: frequency and associated factors in cats in northeastern Brazil

Lacerda¹,

Silva²,

Freitas³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Our aims were to determine the frequencies of feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) in owned and stray cats in the northeastern region of Brazil, ascertain the status of FeLV infection, and investigate potential associated factors among the owned cats. Blood samples from 200 asymptomatic owned cats and 30 stray cats were processed using nested PCR and commercial immunochromatographic tests to diagnose infections. To evaluate the factors associated with FIV and/or FeLV in owned cats, a semistructured interview was conducted with each owner about the animal's environment, and these data were subjected to unconditional logistic regression. The frequencies for owned cats were 6% (12/200) and 3% (6/200) for FIV and FeLV, respectively. No owned cat was positive for both viruses. Stray cats showed frequencies of 6.66% (2/30) and 0% (0/30) for FIV and FeLV, respectively. Contact with other cats and living in peri-urban areas were considered to be risk factors (P < 0.05) for FIV. We did not identify any factors associated with infections with 2 L.C. Lacerda et al. Genetics and Molecular Research 16 (2): gmr16029633FeLV. Our results confirm the presence of these two retroviruses in the region under study. Our use of different diagnostic techniques allowed us to determine the frequency of retroviruses in the feline population more accurately, particularly with regard to infections by FeLV, which have complex pathogenesis.

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Section: Introductionmentioning

confidence: 99%

Feline immunodeficiency virus and feline leukemia virus: frequency and associated factors in cats in northeastern Brazil

Lacerda¹,

Silva²,

Freitas³

et al. 2017

Genet. Mol. Res.

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“…With the advent of molecular diagnostics, conventional PCR to detect FeLV DNA was employed in attempt to better understand these discrepant results (Jackson et al, 1996;Miyazawa and Jarrett, 1997;Herring et al, 2001), but the assay did not appear to have increased sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Development and application of a quantitative real-time PCR assay to detect feline leukemia virus RNA

Torres¹,

O’Halloran²,

Larson³

et al. 2008

Veterinary Immunology and Immunopathology

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We previously defined four categories of FeLV infection, designated as abortive, regressive, latent, and progressive. To determine if detectable viral DNA is transcriptionally active in the absence of antigenemia, we developed and validated a real-time viral RNA qPCR assay. This assay proved to be highly sensitive, specific, reproducible, and allowed reliable quantitation. We then applied this methodology, together with real-time DNA qPCR and p27 capsid antigen capture ELISA, to examine cats challenged with FeLV. We found that circulating viral RNA and DNA levels were highly correlated and the assays were almost in perfect agreement. This indicates that the vast majority of viral DNA is transcriptionally active, even in the absence of antigenemia. The real-time qPCR assays are more sensitive than the most commonly used FeLV diagnostic assay, the p27 capsid antigen capture ELISA. Application of qPCR assays may add greater depth in understanding of FeLV-host relationships.

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“…2,11 Most FeLV-positive cats have progressive viremia with continuous shedding of the virus in saliva. 2,12 Detection of proviral DNA by polymerase chain reaction (PCR) is indicated in cases of suspected regressive infection, where ELISA and IFA results are negative. 1,12,13 In Brazil, only a few FeLV epidemiologic studies have been carried out with small numbers of samples from domestic cats tested by immunochromatographic assays.…”

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confidence: 99%

Prevalence of feline leukemia virus infection in domestic cats in Rio de Janeiro

Almeida

Danelli

Silva

et al. 2012

Journal of Feline Medicine and Surgery

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Peripheral blood smears of 1094 domestic cats were collected and tested by indirect immunofluorescence antibody assay for p27 antigen in cells to study the prevalence and risk factors for feline leukemia virus (FeLV) in the state of Rio de Janeiro. Sex, age, breed, outdoor access, neutering status, type of habitation (household, shelter, veterinary clinics and other places), number of household cats and clinical signs were registered on a form. Among the tested samples, 11.52% were positive. Risk factors for FeLV infection included outdoor access, age range between 1 and 5 years old, and cohabitation with numerous cats.

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Detection of Feline Leukaemia Virus in Blood and Bone Marrow of Cats with Varying Suspicion of Latent Infection

Cited by 22 publications

References 19 publications

Feline immunodeficiency virus and feline leukemia virus: frequency and associated factors in cats in northeastern Brazil

Feline immunodeficiency virus and feline leukemia virus: frequency and associated factors in cats in northeastern Brazil

Development and application of a quantitative real-time PCR assay to detect feline leukemia virus RNA

Prevalence of feline leukemia virus infection in domestic cats in Rio de Janeiro

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