Detection of fentanyl and derivatives using a lateral flow immunoassay

Angelini, Daniel J.; Biggs, Tracey D.; Prugh, Amber; Smith, Jessica A.; Hanburger, Jennifer A.; Llano, Bob; Avelar, Raquel; Ellis, Angela E.; Lusk, Brady; Naanaa, Abdallah Malik; Feasel, Michael G.; Sekowski, Jennifer W.

doi:10.1016/j.forc.2021.100309

Cited by 17 publications

(12 citation statements)

References 27 publications

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“…These results indicate that this combination of these three aptamers can broadly detect fentanyl and its analogues in various seized substances with broad cross-reactivity. In contrast, nine different previously studied commercially available immunoassays could detect furanyl fentanyl, valeryl fentanyl, and p -fluoroisobutyryl fentanyl with a cross-reactivity of <13% and could not detect p -methoxy butyryl fentanyl, cis -3-methyl fentanyl, and p -fluorofentanyl at all. − …”

Section: Resultsmentioning

confidence: 86%

Suite of Aptamer-Based Sensors for the Detection of Fentanyl and Its Analogues

et al. 2023

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Fentanyl and its analogues are potent synthetic opioids that are commonly abused and are currently the number one cause of drug overdose death in the United States. The ability to detect fentanyl with simple, rapid, and low-cost tools is crucial for forensics, medical care, and public safety. Conventional on-site testing options for fentanyl detectionincluding chemical spot tests, lateral-flow immunoassays, and portable Raman spectrometerseach have their own unique flaws that limit their analytical utility. Here, we have developed a series of new aptamer-based assays and sensors that can detect fentanyl as well as several of its analogues in a reliable, accurate, rapid, and economic manner. These include colorimetric, fluorescent, and electrochemical sensors, which can detect and quantify minute quantities of fentanyl and many of its analogues with no response to other illicit drugs, cutting agents, or adulterantseven in interferent-ridden binary mixtures containing as little as 1% fentanyl. Given the high performance of these novel analytical tools, we foresee the potential for routine use by medical and law enforcement personnel as well as the general public to aid in rapid and accurate fentanyl identification.

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Section: Resultsmentioning

confidence: 86%

Suite of Aptamer-Based Sensors for the Detection of Fentanyl and Its Analogues

et al. 2023

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“…The hydrogel material (Hydrogel DG‐1108) used for this study was obtained from KT Chemicals, Inc. (Richardson, TX) [25]. The polycarbonate surfaces (0.125″ thickness, 2″ × 2″) were obtained from the Advanced Design and Manufacturing Branch of the U.S. Army Combat Capabilities Development Command (DEVCOM) Chemical Biological Center (Edgewood Area‐Aberdeen Proving Ground) [26]. Human Jurkat genomic DNA (ThermoFisher Scientific) was used as the experimental DNA for this study.…”

Section: Methodsmentioning

confidence: 99%

Use of a commercially available hydrogel as a novel DNA surface sampling tool

Sekowski

Simmons

Lal-Paterson

et al. 2023

Journal of Forensic Sciences

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A common requirement in the military, law enforcement, and forensic mission space is the need to collect trace samples from surfaces using a method that not only readily captures the sample but also retains its integrity for downstream identification and characterization. Additionally, collecting samples from three-dimensional objects (e.g., shell casings) is a challenge for which there is currently no validated standardized approach. Recently, hydrogels have been shown to have the potential for surface collection of trace bacterial spores, amino acids, and DNA. To test whether these hydrogels can serve as a viable collection medium for sampling DNA from surfaces, we carried out a series of preliminary tests examining collection efficiency and suitability of hydrogel material to recover samples of diluted, dried human DNA on a smooth polycarbonate surface. The recovery of surface DNA using a commercially available hydrogel was examined, and the efficiency compared to samples collected using a standard foam collection swab. DNA collected using the hydrogel and swab methods was then examined using quantitative polymerase chain reaction (qPCR) and short tandem repeat (STR) analysis to determine whether the collection material was compatible with these downstream processes. The hydrogel material used for this study collected the experimental DNA with comparable efficiency to standard collection swabs. In addition, qPCR and STR analyses demonstrated compatibility with the hydrogel collection and extraction process. These data suggest that hydrogels have the potential to be used as sample collection materials and deserve further characterization to elucidate their utility in collection from irregularly shaped, three-dimensional surfaces/materials.

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“…A review of the literature indicates the value of portable methods. Evaluations of immunoassay kits indicate the method is useful for the detection of fentanyl and some of its analogs [23][24][25][26][27]. These studies evaluated these substances in a variety of liquid matrices and included results of experiments performed in the presence of interferents.…”

Section: Highlightsmentioning

confidence: 99%

Evaluation of portable gas chromatography–mass spectrometry (GC–MS) for the analysis of fentanyl, fentanyl analogs, and other synthetic opioids

Leary,

Kizzire,

Chan Chao

et al. 2023

Journal of Forensic Sciences

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Potent synthetic opioids including fentanyl and its analogs are frequently encountered in the field and require detection and identification by first responders to maintain the safety of drug abusers, first responders, health‐care providers, and the public at large. Due to the low concentration at which these substances may be encountered and the complicating matrices within which they may be dispersed, the use of portable gas chromatography–mass spectrometry (GC–MS) for their identification in the field offers great potential value. This research established that portable GC–MS is a useful method for the detection and identification of a large number of synthetic opioids, especially fentanyl and its analogs. In this study, 250 synthetic opioids and related substances including 210 fentanyl analogs were analyzed using portable GC–MS. It was concluded that 225 of the 250 (90.0%) opioids analyzed were successfully detected onboard at the time of analysis and identified as either the substance (55.2%) or an analog (34.8%). These outcomes have equivalent benefit for the field analysis of illicit drugs due to both initiating the same subsequent actions by first responders.

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Detection of fentanyl and derivatives using a lateral flow immunoassay

Cited by 17 publications

References 27 publications

Suite of Aptamer-Based Sensors for the Detection of Fentanyl and Its Analogues

Suite of Aptamer-Based Sensors for the Detection of Fentanyl and Its Analogues

Use of a commercially available hydrogel as a novel DNA surface sampling tool

Evaluation of portable gas chromatography–mass spectrometry (GC–MS) for the analysis of fentanyl, fentanyl analogs, and other synthetic opioids

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