Detection of Florfenicol Residues in Salmon Trout via GC–MS

Yikilmaz, Yeliz; Filazı, Ayhan

doi:10.1007/s12161-014-9982-8

Cited by 24 publications

(6 citation statements)

References 36 publications

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“…Compared to ethyl acetate, acetonitrile was stronger polar solvent, which could extract more polar compounds, leading to more matrix interferences and ion suppression for the polar target analytes, especially amitrole . As discussed above, ethyl acetate was used for extraction of the analytes from fish samples, which has been applied for diethylstilbestrol , florfenicol , and other chemicals from fish.…”

Section: Resultsmentioning

confidence: 99%

Determination of a broad spectrum of endocrine-disrupting pesticides in fish samples by UHPLC-MS/MS using the pass-through cleanup approach

et al. 2017

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We describe a new methodology for the simultaneous determination of the endocrine-disrupting herbicides (acetochlor, alachlor, amitrole, and atrazine), fungicides (carbendazim, triadimefon, penconazole, and propiconazole), and insecticides (carbaryl and carbofuran) in fish samples followed by high-performance liquid chromatography with tandem mass spectrometry. Samples were extracted and purified using the pass-through cleanup approach. The recoveries of the pesticides were in the range 71.8-116.5%, with relative standard deviations lower than 15.28%. Limits of quantitation were in the range of 0.03-2.50 μg/kg. Validation results on linearity, accuracy, and precision, as well as on application to the analysis of the endocrine-disrupting pesticides in 20 fish samples, demonstrated the applicability to screen the presence of pesticides in fish.

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Section: Resultsmentioning

confidence: 99%

Determination of a broad spectrum of endocrine-disrupting pesticides in fish samples by UHPLC-MS/MS using the pass-through cleanup approach

et al. 2017

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“…In recent years, several analytical methods based on high-performance liquid chromatography (HPLC) [7,9], high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) [3,[10][11][12][13], gas chromatography (GC) [14], and gas chromatography-mass spectrometry (GC-MS) [15] have been reported for the determination of FF and its metabolites in water, feed, and animal-derived food. On the other hand, the analysis of animal biological fuids has been less frequent, mainly involving serum [16], plasma [13,17], synovial fuid [18], and cerebrospinal fuid [19], but no study has focused on the pharmacokinetics of FF and FFA in seminal plasma.…”

Section: Introductionmentioning

confidence: 99%

Florfenicol and Florfenicol Amine Quantification in Bull Serum and Seminal Plasma by a Single Validated UHPLC‐MS/MS Method

Bardhi

Romano

Pagliuca

et al. 2023

Veterinary Medicine International

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Florfenicol is a broad-spectrum antibiotic belonging to the amphenicols class that inhibits protein synthesis by binding to bacteria's ribosomal subunits. This drug is commonly used in veterinary medicine to treat bacterial infectious diseases in cattle, swine, poultry, and fish. The proposed method uses a quick protein precipitation with acetonitrile for the extraction of florfenicol and florfenicol amine in serum and seminal plasma, followed by analysis in UHPLC-MS/MS for their simultaneous quantification. A BEH C18 reversed-phase column was chosen for analyte separation, allowing to obtaining sharp and symmetrical peak shapes in a chromatographic run of just 3.5 min under programmed conditions. Two specific transitions were observed for each analyte, and florfenicol-d3 was used as the internal standard. The approach was fully validated in each matrix over ranges suitable for field concentrations of florfenicol and florfenicol amine, showing good linearity during each day of testing (R2 always >0.99). Excellent accuracy and precision were demonstrated, for both analytes, by calculated bias always within ±15% and CV% always below 15% at all QC levels tested. The satisfactory outcomes obtained during recovery, matrix effect, and process efficiency investigations in serum and seminal plasma confirmed the strength of the method for the quantification of target compounds. To our knowledge, this is the first LC-MS/MS-validated approach for the quantification of florfenicol and florfenicol amine in serum and seminal plasma and was successfully applied for the determination of their concentration-time profiles in bulls. This paves the way to understanding the pharmacokinetics of this antibiotic and its active metabolite in bull’s seminal plasma, which will enable the design of more appropriate treatment protocols.

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“…A variety of amphenicol detection approaches have been extensively developed by immunosorbent assay [ 11 , 12 ], fluorescence sensors [ 13 , 14 , 15 ], electrochemical sensors [ 16 , 17 , 18 , 19 ], gas chromatography–mass spectrometry (GC/MS) [ 20 , 21 , 22 ], and high-performance liquid chromatography–mass spectrometry (HPLC–MS/MS), etc. [ 23 , 24 , 25 , 26 , 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Determination of Antibiotic Residues in Aquatic Products by High-Performance Liquid Chromatography–Tandem Mass Spectrometry

et al. 2022

Antibiotics

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Antibiotic drug residues are crucial to ensure food safety and minimize risk to human health. Herein, a sensitive high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed and validated for the determination of antibiotic residues (mainly amphenicols) consisting of chloramphenicol (CAP), thiamphenicol (TAP), florfenicol (FF), and florfenicol amine (FFA) in aquatic products. Amphenicols were well separated on a Kinetex F5 (100 mm × 3.0 mm, 2.6 µm) chromatographic column with the mobile phases of 1 mM ammonium acetate aqueous solution and methanol solution and measured after positive and negative electrospray ionizations using four internal standards. To our knowledge, it was the first time to report the good performance of F5 column and four internal standards for the determination of amphenicols. The established method featured a good linear relationship between chromatographic peak area ratios and the concentrations of amphenicols (R2 > 0.992), a wide and low detection matrix-based range of 0.01-5 μg/L, a low detection limit of 0.01 μg/kg, etc. The spiked assays evidenced the accuracy and reliability of the developed method with the recoveries between 84.0 and 105 %, the intraday relative standard deviations (RSDs) over the range of 0.769-13.7 %, and the interday RSDs over the range of 0.582-13.3 %. Finally, the proposed method was applied to investigate amphenicol residues in various aquatic products, including fish, shrimp, crab, shellfish, and other aquatic species.

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Detection of Florfenicol Residues in Salmon Trout via GC–MS

Cited by 24 publications

References 36 publications

Determination of a broad spectrum of endocrine-disrupting pesticides in fish samples by UHPLC-MS/MS using the pass-through cleanup approach

Determination of a broad spectrum of endocrine-disrupting pesticides in fish samples by UHPLC-MS/MS using the pass-through cleanup approach

Florfenicol and Florfenicol Amine Quantification in Bull Serum and Seminal Plasma by a Single Validated UHPLC‐MS/MS Method

Highly Sensitive Determination of Antibiotic Residues in Aquatic Products by High-Performance Liquid Chromatography–Tandem Mass Spectrometry

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