Detection of fractalkine in human seminal plasma and its role in infertile patients

Zhang, Qing; Shimoya, Koichiro; Ohta, Yukinibu; Chin, Rika; Tenma, Kumiko; Isaka, Shigeyuki; Nakamura, Hitomi; Koyama, Masayasu; Azuma, Chihiro; Murata, Yuji

doi:10.1093/humrep/17.6.1560

Cited by 9 publications

(13 citation statements)

References 29 publications

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“…CXCL10 is able to inhibit progesterone synthesis in Leydig cells and proliferation of these cells 54 . The effect of CXCL8 on spermatozoal motility is unclear, but the high seminal plasma CXCL8 concentration was reported in the men with leukocytospermia 56 as well as the low CX 3 CL1 concentration in the subfertile men with asthenozoospermia 57 . These findings are in consistent with the results in murine autoimmune orchitis models 58 .…”

Section: Pathophysiological Roles Of Chemokines In Human Reproductionsupporting

confidence: 80%

Pathophysiological Roles of Chemokines in Human Reproduction: An Overview

Kitaya

Yamada

2010

American Journal of Reproductive Immunology

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Chemokines are a group of small cytokines that have an ability to induce leukocyte migration. Chemokines exert their functions by binding and activating specific G protein-coupled receptors. Studies have unveiled pleiotropic bioactivities of chemokines in various phenomena ranging from immunomodulation, embryogenesis, and homeostasis to pathogenesis. In the mammalian reproductive system, chemokines unexceptionally serve in multimodal events that are closely associated with establishment, maintenance, and deterioration of fecundity. The aim of this review is to update the knowledge on chemokines in male and female genital organs, with a focus on their potential pathophysiological roles in human reproduction.

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Section: Pathophysiological Roles Of Chemokines In Human Reproductionsupporting

confidence: 80%

Pathophysiological Roles of Chemokines in Human Reproduction: An Overview

Kitaya

Yamada

2010

American Journal of Reproductive Immunology

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“…In recent years, several reports have attempted to correlate different SP protein levels and semen parameters, such as sperm concentration, motility, and morphology (Baumgart et al 2002, Zhang et al 2002. We followed this objective in order to determine the possible diagnostic validity of MSMB concentration as a biochemical marker that could provide useful information on male fertility potential.…”

Section: B-microseminoprotein In Human Spermmentioning

confidence: 99%

β-Microseminoprotein in human spermatozoa and its potential role in male fertility

Franchi

Avendaño²,

Molina³

et al. 2008

Reproduction

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Abstractb-Microseminoprotein (MSMB) is one of the most abundant proteins in human seminal plasma. The objectives of this study were: (1) to purify MSMB from seminal plasma (SP) and generate antibodies against the pure protein; (2) to investigate the interaction of MSMB with ejaculated spermatozoa and its possible effect on the spontaneous acrosome reaction (AR); and (3) to quantify MSMB content in SP and examine its relationship with the clinical sperm parameters. MSMB was purified from SP and its presence on the sperm surface was examined by indirect immunofluorescence using a specific polyclonal antibody. The effect of MSMB on the AR was evaluated using guinea pig epididymal spermatozoa as a model. MSMB quantification assay was performed with a two-site binding ELISA using two polyclonal antibodies against MSMB. MSMB was assessed in semen samples from fertile donors (controls) and subfertile patients according to World Health Organization criteria. MSMB was detected on the sperm surface and mainly localized to the acrosomal region of the head and neck. A significant spontaneous AR inhibition was observed when guinea pig epididymal spermatozoa were preincubated with MSMB. Finally, MSMB was significantly increased in subfertile patients when compared with fertile controls (P!0.02). The association of MSMB to the sperm surface, the inhibitor effect on the spontaneous AR and the increased MSMB levels found in SP in subfertile men suggests a relationship between this protein and semen quality and a possible role in the process of fertilization. Reproduction (2008) 136 157-166

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“…RT-PCR was performed using an RT-PCR high kit (TOYOBO Co., Osaka, Japan), as previously described [10][11][12]. Amplification using CX 3 CR1-specific primers yielded a 653-bp DNA product, while amplification using the fractalkine-specific primers yielded a 597-bp DNA product (5).…”

Section: Rt-pcr Amplificationmentioning

confidence: 99%

“…CX 3 CR1-mediated signal transduction presumably plays a role in cell migration and adhesion [8,9]. In the reproductive system, this molecule is present in the seminal plasma and is associated with sperm motility [10]. We reported that fractalkine contributes to the immunodefense mechanism during pregnancy [11] and that this molecule is present in the Fallopian tube and that its receptor exists in the spermatozoa [12].…”

Section: Introductionmentioning

confidence: 98%