Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR

Dutka‐Malen, Sylvie; Evers, Stefan; Courvalin, Patrice

doi:10.1128/jcm.33.1.24-27.1995

Cited by 1,184 publications

(421 citation statements)

References 35 publications

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“…A low resistance was found in the strain identified as Ent. gallinarum , as was expected for this species, which is known to possess a constitutive low‐level resistance to this antibiotic ( Dutka‐Malen et al. 1995 ).…”

Section: Discussionsupporting

confidence: 65%

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A survey of the enterococci isolated from an artisanal Italian goat's cheese (semicotto caprino)

et al. 2000

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. Enterococci were isolated from semicotto caprino cheese, a traditional cheese produced in Southern Italy: they were a signi®cant part of the microbial population of this cheese, con®rming the importance of the presence of these micro-organisms during cheese-making and ripening. They were also identi®ed and studied for their phenotypic and genotypic characteristics: Enterococcus faecalis and Ent. faecium were the most frequently isolated species, followed by Ent. durans, Ent. hirae and Ent. gallinarum. None of the isolates showed lipolytic activity, whereas they were characterized by a relevant proteolytic activity as well as an antagonistic activity towards Listeria innocua. One strain of Ent. gallinarum showed a low-level resistance to vancomycin, while six out of the 79 Ent. faecalis strains possessed b-haemolysis reaction. The highest acidifying potential in skim milk was obtained by Ent. faecalis isolates. Thirty enterococcal strains representative of the different species at different ripening times were analysed by means of RAPD-PCR, and revealed species-speci®c pro®les for all the considered species.

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Section: Discussionsupporting

confidence: 65%

“…gallinarum on the basis of its RAPD profile. When tested with the species‐specific primers described by Dutka‐Malen et al. (1995) , this isolate was identified as Ent.…”

Section: Resultsmentioning

confidence: 99%

A survey of the enterococci isolated from an artisanal Italian goat's cheese (semicotto caprino)

et al. 2000

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“…The final extension lasted for 10 min at 72°C. PCR was also used for detection of the vanB gene in accordance with previous descriptions (Dutka‐Malen et al. 1995).…”

Section: Methodsmentioning

confidence: 99%

Cross-transmission of clinicalEnterococcus faeciumin relation toespand antibiotic resistance

Billström

Sullivan

Lund

2008

Journal of Applied Microbiology

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Aims: To investigate clonality among clinical Enterococcus faecium isolates and normal intestinal microflora isolates as well as cross‐transmission between patients in relation to the presence of the esp gene and antibiotic resistance. Methods and Results: Blood‐culture isolates (n = 101) deriving from tertiary, secondary and primary hospitals were analysed. Antibiotic susceptibility was investigated. Polymerase chain reaction and pulsed‐field gel electrophoresis were used for detection of esp and genotyping, respectively. Nearly half (43%) of the patients included were involved in a cross‐transmission event with Ent. faecium. These strains disseminated both within and between all hospitals. The antibiotic resistance and presence of esp were highest in isolates from the tertiary hospital. Isolates harbouring esp showed less genetic diversity compared with esp negative ones. Conclusions: Cross‐transmission with Ent. faecium between patients was readily detected, indicating that hospital‐adapted clones circulate within and between hospitals. Acquired characteristics, such as antibiotic resistance and esp, seem to accumulate in the isolates disseminating in the tertiary hospital. Significance and Impact of the Study: It is important to characterize Ent. faecium isolates causing infections and to determine the extent of dissemination in order to prevent further spread of these pathogens.

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“…During 2 years, E. faecalis isolates were collected from patients with clinical symptoms of UTIs at two universityaffiliated hospitals (Kerman, Iran). Following identification and confirmation, 7,8 disk diffusion test was performed to detect susceptibility, in order to find MDR isolates (i.e. resistance to more than three different classes of antibiotics).…”

Section: Bacterial Population and Antimicrobial Susceptibility Testingmentioning

confidence: 99%

<p>Co-Incidence of Type II Topoisomerase Mutations and Efflux Expression in High Fluoroquinolone Resistant <em>Enterococcus faecalis</em> Isolated from Urinary Tract Infections</p>

Esfahani¹,

Ahmadrajabi²,

Mollaie³

et al. 2020

IDR

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Introduction: Enterococcus faecalis is one of the most common pathogens in urinary tract infections (UTIs). Fluoroquinolones have been frequently used to treat E. faecalis UTIs, and the emergence of fluoroquinolone-resistant E. faecalis strains has recently been reported in several countries. This study aimed to elucidate the mechanisms involved in fluoroquinolone resistance in clinical E. faecalis isolates by analyzing mutations in quinolone-resistance-determining regions (QRDRs) of gyrA and parC and investigating the role of some efflux pumps. Methods: In total, 70 clinical E. faecalis isolates collected from UTIs were identified by phenotypic and genotypic methods. Antimicrobial susceptibility testing was performed and multidrug-resistant (including ciprofloxacin resistant) isolates were studied for minimum inhibitory concentrations to ciprofloxacin, levofloxacin, and ofloxacin. In the following, mutations in QRDRs of gyrA and parC and expression of EfrA, EfrB, and EmeA efflux pumps were investigated in 20 high-level ciprofloxacin resistant and two ciprofloxacin susceptible isolates. Results: High-level resistance to ciprofloxacin was detected in 97.5% of isolates. Sequencing of QRDRs revealed that 65% and 75% of isolates carried mutations in gyrA and parC, respectively. The presence of efflux genes was detected in all studied isolates, but expression of efrA, emeA, and efrB was demonstrated in 50%, 40%, and 30% of resistant isolates, respectively. Neither QRDR mutation nor the expression of efflux genes showed any significant association with MIC. Conclusion: Co-incidence of mutation and efflux gene expression in more than half of isolates (13/20) suggests that both mechanisms may play a role in fluoroquinolone resistance. The other unknown mechanisms including different efflux pumps and probably other QRDRs mutations may contribute to fluoroquinolone resistance in E. faecalis.

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Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR

Cited by 1,184 publications

References 35 publications

A survey of the enterococci isolated from an artisanal Italian goat's cheese (semicotto caprino)

A survey of the enterococci isolated from an artisanal Italian goat's cheese (semicotto caprino)

Cross-transmission of clinicalEnterococcus faeciumin relation toespand antibiotic resistance

<p>Co-Incidence of Type II Topoisomerase Mutations and Efflux Expression in High Fluoroquinolone Resistant <em>Enterococcus faecalis</em> Isolated from Urinary Tract Infections</p>

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