Detection of glycosyltransferases in the golden hamster (Mesocricetus auratus) oviduct and evidence for the regulation of O-glycan biosynthesis during the estrous cycle

McBride, Deborah S.; Brockhausen, Inka; Kan, Frederick W. K.

doi:10.1016/j.bbagen.2004.07.009

Cited by 14 publications

(7 citation statements)

References 34 publications

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“…Similar observations were reported for recombinant glycosylated human OVGP1 expressed in HEK293 cells where tyrosine phosphorylation of sperm proteins was enhanced, and the number of acrosome-reacted sperms was significantly increased [ 48 ]. It was reported that glycosylation differences detected in the estrous cycle could be responsible for a different biological role of the oviductal-secreted glycoproteins in golden hamster [ 49 , 50 ]. In the present study, the recombinant OVGP1 expressed in E. coli lacks the carbohydrate moieties, and this may be responsible for the variations observed in the effects of native and recombinant OVGP1 on different parameters of sperms i.e.…”

Section: Discussionmentioning

confidence: 99%

Effect of recombinant and native buffalo OVGP1 on sperm functions and in vitro embryo development: a comparative study

Choudhary

Kumaresan

Kumar

et al. 2017

J Animal Sci Biotechnol

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BackgroundAn oviduct- specific glycoprotein, OVGP1, is synthesized and secreted by non-ciliated epithelial cells of the mammalian oviduct which provides an essential milieu for reproductive functions. The present study reports the effects of recombinant buffalo OVGP1 that lacks post-translational modifications, and native Buffalo OVGP1 isolated from oviductal tissue, on frozen- thawed sperm functions and in vitro embryo development.ResultsThe proportion of viable sperms was greater (P < 0.05) in the recombinant OVGP1-treated group compared to the native OVGP1-treated group at 2 h, 3 h, and 4 h of incubation. The proportion of motile sperms at 3 h and 4 h of incubation; and membrane- intact sperms at 4 h was greater (P < 0.05) in the native OVGP1-treated group compared to the control and recombinant OVGP1-treated groups. The proportion of capacitated and acrosome- reacted sperms was greater (P < 0.05) in the native OVGP1-treated group compared to the recombinant OVGP1 group at 4 h. The rates of cleavage of embryos and their development to the blastocyst stage were greater (P < 0.05) in the presence of either native or recombinant OVGP1 in comparison to control at 10 μg/mL concentration as compared to 5 or 20 μg/mL.ConclusionsThe study suggests that both native and recombinant OVGP1 impart a positive effect on various sperm features and in vitro embryo development. However, native OVGP1 was found to have a more pronounced effect in comparison to recombinant non-glycosylated OVGP1 on various sperm functions except viability. Hence, our current findings infer that glycosylation of OVGP1 might be essential in sustaining the sperm functions but not the in vitro embryo development.Electronic supplementary materialThe online version of this article (doi:10.1186/s40104-017-0201-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Effect of recombinant and native buffalo OVGP1 on sperm functions and in vitro embryo development: a comparative study

Choudhary

Kumaresan

Kumar

et al. 2017

J Animal Sci Biotechnol

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“…However, GCNT3 expression was lowest at estrus, indicating that levels of Cores 2 and 4 may fall as mucin synthesis rises. In murine oviduct, the opposite expression pattern has been demonstrated for the ortholog of this gene (55). Transcripts encoding core synthase 3 were expressed at a very low level (Ͻ2 TPM) in bovine cervix and were excluded from our analysis.…”

Section: Discussionmentioning

confidence: 99%

Molecular aspects of mucin biosynthesis and mucus formation in the bovine cervix during the periestrous period

Pluta

McGettigan

Reid

et al. 2012

Physiological Genomics

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Mucus within the cervical canal represents a hormonally regulated barrier that reconciles the need to exclude the vaginal microflora from the uterus during progesterone dominance, while permitting sperm transport at estrus. Its characteristics change during the estrous cycle to facilitate these competing functional requirements. Hydrated mucin glycoproteins synthesized by the endocervical epithelium form the molecular scaffold of this mucus. This study uses the bovine cervix as a model to examine functional groups of genes related to mucin biosynthesis and mucus production over the periestrous period when functional changes in cervical barrier function are most prominent. Cervical tissue samples were collected from 30 estrus synchronized beef heifers. Animals were slaughtered in groups starting 12 h after the withdrawal of intravaginal progesterone releasing devices (controlled internal drug releases) until 7 days postonset of estrus (luteal phase). Subsequent groupings represented proestrus, early estrus, late estrus, metestrus, and finally the early luteal phase. Tissues were submitted to next generation RNA-seq transcriptome analysis. We identified 114 genes associated with biosynthesis and intracellular transport of mucins, and postsecretory modifications of cervical; 53 of these genes showed at least a twofold change in one or more experimental group in relation to onset of estrus, and the differences between groups were significant (P < 0.05). The majority of these genes showed the greatest alteration in their expression in the 48 h postestrus and luteal phase groups.

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“…Most of the carbohydrates are present in O‐linked chains but N‐linked chains have also been detected (Malette & Bleau, 1993). Glycosylation differences detected over the oestrous cycle could be responsible for a different biological role of oviductal‐secreted glycoproteins (McBride et al , 2004; McBride Brockhausen, & Kan, 2005). Third, OVGP1 activity could be affected by ZP composition.…”

Section: Pre‐fertilisation Zona Pellucida Hardeningmentioning

confidence: 99%

What controls polyspermy in mammals, the oviduct or the oocyte?

Coy

Sánchez

2010

Biological Reviews

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A block to polyspermy is required for successful fertilisation and embryo survival in mammals. A higher incidence of polyspermy is observed during in vitro fertilisation (IVF) compared with the in vivo situation in several species. Two groups of mechanisms have traditionally been proposed as contributing to the block to polyspermy in mammals: oviduct-based mechanisms, avoiding a massive arrival of spermatozoa in the proximity of the oocyte, and egg-based mechanisms, including changes in the membrane and zona pellucida (ZP) in reaction to the fertilising sperm. Additionally, a mechanism has been described recently which involves modifications of the ZP in the oviduct before the oocyte interacts with spermatozoa, termed "pre-fertilisation zona pellucida hardening". This mechanism is mediated by the oviductal-specific glycoprotein (OVGP1) secreted by the oviductal epithelial cells around the time of ovulation, and is reinforced by heparin-like glycosaminoglycans (S-GAGs) present in oviductal fluid. Identification of the molecules contributing to the ZP modifications in the oviduct will improve our knowledge of the mechanisms of sperm-egg interaction and could help to increase the success of IVF systems in domestic animals and humans.

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Detection of glycosyltransferases in the golden hamster (Mesocricetus auratus) oviduct and evidence for the regulation of O-glycan biosynthesis during the estrous cycle

Cited by 14 publications

References 34 publications

Effect of recombinant and native buffalo OVGP1 on sperm functions and in vitro embryo development: a comparative study

Effect of recombinant and native buffalo OVGP1 on sperm functions and in vitro embryo development: a comparative study

Molecular aspects of mucin biosynthesis and mucus formation in the bovine cervix during the periestrous period

What controls polyspermy in mammals, the oviduct or the oocyte?

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