Effect of recombinant and native buffalo OVGP1 on sperm functions and in vitro embryo development: a comparative study

Choudhary, Suman; Kumaresan, A.; Kumar, Manish; Chhillar, Shivani; Malik, Hruda Nanda; Kumar, Sudarshan; Kaushik, Jai K.; Datta, Tirtha Kumar; Mohanty, Ashok Kumar

doi:10.1186/s40104-017-0201-5

Cited by 25 publications

(23 citation statements)

References 48 publications

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“…Recently, a study in water buffalo also reported that there was no difference in terms of blastocyst production rate following IVF with frozen-thawed sperm of a cloned Murrah bull and its donor [ 6 ], although they did not examine cleavage rates and quality of produced blastocysts. This study had low blastocyst rates (~10%), which is in agreement with other IVF studies in buffalos [ 29 – 32 ]. The laboratory-to-laboratory variations, conditions, protocols, and other factors could have contributed variation in IVF results [ 33 ].…”

Section: Discussionsupporting

confidence: 92%

Semen parameters and fertility potency of a cloned water buffalo (Bubalus bubalis) bull produced from a semen-derived epithelial cell

et al. 2020

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Semen contains epithelial cells that can be cultured in vitro . For somatic cell nuclear transfer applications, it is essential to know whether clone(s) produced from semen-derived epithelial cells (SedECs) are healthy and reproductively competent. In this study, the semen and fertility profile of a cloned bull (C1) that was produced from a SedEC were compared with its donor (D1) and with two cloned bulls (C2, C3) that were produced from commonly used skin-derived fibroblast cells (SkdFCs). We observed variations in some fresh semen parameters (ejaculated volume and mass motility), frozen-thawed sperm parameters (plasma membrane integrity, and computer-assisted semen analysis (CASA) indices), but values are within the normal expected range. There was no difference in sperm concentration of ejaculated semen and frozen-thawed semen parameters which include sperm motility, percentage of live and normal morphology sperm, and distance traveled through oestrus mucus. Following in vitro fertilization (IVF) experiments, zygotes from C1 had higher ( P < 0 . 05 ) cleavage rates (81%) than C2, C3, and D1 (71%, 67%, and 75%, respectively); however, blastocyst development per cleaved embryo and quality of produced blastocysts did not differ. The conception rate of C1 was 46% (7/15) and C2 was 50% (8/15) following artificial insemination with frozen-thawed semen. Established pregnancies resulted in births of 7 and 6 progenies sired by C1 and C2, respectively, and all calves show no signs of phenotypical abnormalities. These results showed that semen from a cloned bull derived from SedECs is equivalent to semen from its donor bull and bulls cloned from SkdFCs.

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Section: Discussionsupporting

confidence: 92%

Semen parameters and fertility potency of a cloned water buffalo (Bubalus bubalis) bull produced from a semen-derived epithelial cell

et al. 2020

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“…Some roles played by oviductin on early embryo development were reported from in vitro studies. Consistent positive effects of purified oviductin were observed on blastocyst yield in goat [17], sheep [40], pig [41] and cattle [18]. Antibodies directed against the C-terminal peptide of rabbit oviductin were shown to inhibit mouse embryo development at the 2-cell stage, suggesting that in this species, oviductin has a function in overcoming the development block at this stage [42].…”

Section: Discussionmentioning

confidence: 71%

“…The proteins present in the OF, either within EVs or as soluble molecules, may play key roles in supporting embryo development. Indeed, the addition of purified or recombinant oviductin, an oviduct-specific estrus-induced glycoprotein, to the culture medium was reported to increase the rates of blastocyst development of goat [17] and cattle [18] embryos compared with non-supplemented controls. In another study, the presence of porcine recombinant oviductin did not enhance in vitro developmental rates but produced bovine blastocysts of higher quality regarding the expression of specific genes [19].…”

Section: Introductionmentioning

confidence: 99%

Identification of 56 Proteins Involved in Embryo–Maternal Interactions in the Bovine Oviduct

Banliat

Tsikis

Labas

et al. 2020

IJMS

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The bovine embryo develops in contact with the oviductal fluid (OF) during the first 4–5 days of pregnancy. The aim of this study was to decipher the protein interactions occurring between the developing embryo and surrounding OF. In-vitro produced 4–6 cell and morula embryos were incubated or not (controls) in post-ovulatory OF (OF-treated embryos) and proteins were then analyzed and quantified by high resolution mass spectrometry (MS) in both embryo groups and in OF. A comparative analysis of MS data allowed the identification and quantification of 56 embryo-interacting proteins originated from the OF, including oviductin (OVGP1) and several annexins (ANXA1, ANXA2, ANXA4) as the most abundant ones. Some embryo-interacting proteins were developmental stage-specific, showing a modulating role of the embryo in protein interactions. Three interacting proteins (OVGP1, ANXA1 and PYGL) were immunolocalized in the perivitelline space and in blastomeres, showing that OF proteins were able to cross the zona pellucida and be taken up by the embryo. Interacting proteins were involved in a wide range of functions, among which metabolism and cellular processes were predominant. This study identified for the first time a high number of oviductal embryo-interacting proteins, paving the way for further targeted studies of proteins potentially involved in the establishment of pregnancy in cattle.

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“…Here, we identified 63 ODF proteins that are important for the early reproductive processes. Some of these proteins, including LTF, HSPA5, and OVGP1 have previously been shown to modify reproductive processes in vitro. Changes in the abundance of diverse immunity proteins, such as CD109 and members of the S100 family, were also induced by insemination.…”

Section: Discussionmentioning

confidence: 99%

Identification of Rabbit Oviductal Fluid Proteins Involved in Pre‐Fertilization Processes by Quantitative Proteomics

Hackenbroch

Meyer

et al. 2019

Proteomics

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Oviductal fluid (ODF) proteins modulate and support reproductive processes in the oviduct. In the present study, proteins involved in the biological events that precede fertilization have been identified in the rabbit ODF proteome, isolated from the ampulla and isthmus of the oviduct at different time points within 8 h after intrauterine insemination. A workflow is used that integrates lectin affinity capture with stable‐isotope dimethyl labeling prior to nanoLC‐MS/MS analysis. In total, over 400 ODF proteins, including 214 lectin enriched glycoproteins, are identified and quantified. Selected data are validated by Western blot analysis. Spatiotemporal alterations in the abundance of ODF proteins in response to insemination are detected by global analysis. A subset of 63 potentially biologically relevant ODF proteins is identified, including extracellular matrix components, chaperones, oxidoreductases, and immunity proteins. Functional enrichment analysis reveals an altered peptidase regulator activity upon insemination. In addition to protein identification and abundance changes, N‐glycopeptide analysis further identifies 281 glycosites on 199 proteins. Taken together, these results show, for the first time, the evolving oviductal milieu early upon insemination. The identified proteins are likely those that modulate in vitro processes, including spermatozoa function.

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Effect of recombinant and native buffalo OVGP1 on sperm functions and in vitro embryo development: a comparative study

Cited by 25 publications

References 48 publications

Semen parameters and fertility potency of a cloned water buffalo (Bubalus bubalis) bull produced from a semen-derived epithelial cell

Semen parameters and fertility potency of a cloned water buffalo (Bubalus bubalis) bull produced from a semen-derived epithelial cell

Identification of 56 Proteins Involved in Embryo–Maternal Interactions in the Bovine Oviduct

Identification of Rabbit Oviductal Fluid Proteins Involved in Pre‐Fertilization Processes by Quantitative Proteomics

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