Semen parameters and fertility potency of a cloned water buffalo (Bubalus bubalis) bull produced from a semen-derived epithelial cell

Abstract

Semen contains epithelial cells that can be cultured in vitro . For somatic cell nuclear transfer applications, it is essential to know whether clone(s) produced from semen-derived epithelial cells (SedECs) are healthy and reproductively competent. In this study, the semen and fertility profile of a cloned bull (C1) that was produced from a SedEC were compared with its donor (D1) and with two cloned bulls (C2, C3) that were produced from commonly used skin-derived fibroblast cells (SkdFCs). We observed variations in some fresh semen parameters (ejaculated volume and mass motility), frozen-thawed sperm parameters (plasma membrane integrity, and computer-assisted semen analysis (CASA) indices), but values are within the normal expected range. There was no difference in sperm concentration of ejaculated semen and frozen-thawed semen parameters which include sperm motility, percentage of live and normal morphology sperm, and distance traveled through oestrus mucus. Following in vitro fertilization (IVF) experiments, zygotes from C1 had higher ( P < 0 . 05 ) cleavage rates (81%) than C2, C3, and D1 (71%, 67%, and 75%, respectively); however, blastocyst development per cleaved embryo and quality of produced blastocysts did not differ. The conception rate of C1 was 46% (7/15) and C2 was 50% (8/15) following artificial insemination with frozen-thawed semen. Established pregnancies resulted in births of 7 and 6 progenies sired by C1 and C2, respectively, and all calves show no signs of phenotypical abnormalities. These results showed that semen from a cloned bull derived from SedECs is equivalent to semen from its donor bull and bulls cloned from SkdFCs.

Development, Quality, and Production Parameters of In Vitro Embryo in Anatolian Water Buffaloes

“…Many researchers have reported that the cleavage rate in the first 24 hours can be considered as the fertilization rate (Beck-Fruchter et al, 2014;Saini et al, 2020) in scientific reports. It was also reported that the IVF rate is lower in buffaloes (45%-50%) compared to cattle (70%) (Sales et al, 2015) (Table 3).…”

“…Moreover, fresh semen, as well as frozen and thawed semen, can be used in the IVF process (Duran et al, 2017;Rubessa et al, 2019). Numerous methods are available for preparing sperm for IVF (Parrish, 2014;Romar et al, 2016;Saini et al, 2020;Volpes et al, 2016). One of the most common mediums used for the processing of semen is the solution of Brackett & Oliphant (BO) (Akter et al, 2023;Smetanina et al, 2019).…”

Sperm quality and fertility of Murrah and Nili-Ravi bulls compared with indigenous buffalo bulls in Bangladesh

“…The overall average pregnancy (%) in buffaloes was found 43.55±4.90 when AI was conducted in estrous synchronized buffalo (kumar et al 2016). The non-return rate following AI using frozen-thawed semen from cloned buffalo bull was found 46-50% (Saini et al 2020). Conception is the nal destination of ejaculated sperm and ovulated oocytes that largely deepens on semen quality, insemination technique, proper timing of AI and other management and environmental (Dalton et al 2010).…”

Multi-OMICS and Molecular Biology Perspective in Buffalo Genome

“…Another group of researchers conducted research using antioxidant micronutrient supplementation in Murrah buffalo, enhancing udder health and milk production [21]. Semen parameter and fertility potency were performed using cloned water buffalo (Bubalus bubalis) in a study [22]. Moreover, various researches were done in India about the effect of progesterone on water buffalo, identification and characterization of traitspecific SNPs using ddRAD sequencing in water buffalo, nanotechnological application in buffalo, etc.…”