The present study was conducted to analyze the fresh and post-thaw semen quality and fertility from native bulls of Red Chittagong Cattle (RCC), BLRI Cattle Breed 1 (BCB1), and Munshiganj Cattle of Bangladesh. One hundred and seventy-two ejaculates were collected by artificial vagina set and semen analysis was performed using Computer Assisted Sperm Analyzer (CASA) at Bangladesh Livestock Research Institute. Commercial extender (AndroMed) was used to dilute the fresh semen. After equilibration (4˚C for 4 hr), freezing was done using a programmable bio-freezer. Post-thawed semen was evaluated for sperm motility and kinematics. Cryopreserved semen straws were used for artificial insemination (AI) and determined the bull fertility based on 60 days non-return rate. Motility of the sperm differs significantly (p < 0.01) among the genotypes. Total motility was higher in Munshiganj bulls and static motility was higher in BCB1 bulls. However, the semen volume and sperm concentration did not vary significantly (p > 0.05) among the bulls but the highest concentration was found in Munshiganj bull (1669.60 ± 192.07 million/ml) followed by RCC (1648.70 ± 91.07 million/ml) and BCB1 bull (1481.60 ± 167.35 million/ml). Moreover, the highest bent tail (5.89 ± 0.75%), coiled tail (1.01% ± 0.22%) and distal mid-piece reflex (2.26% ± 0.28%) were observed in BCB1 followed by Munshiganj and RCC. Amplitude of lateral head displacement (ALH) was recorded higher in post-thaw than in fresh semen. Kinematics parameters of post-thaw semen decreased than fresh semen irrespective of genotypes. More number of doses/ejaculates can be produced from Munshiganj bull (394.34 ± 127.95) followed by RCC (349.01 ± 120.91

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Buffalo semen collected from Murrah bull were cryopreserved and evaluated for different motility parameter, kinematics and plasma membrane integrity. Buffalo bulls were maintained uniform standard management and nutritional practices. Semen was collected regularly twice a week semen collection schedule from four (04) Murrah bull. Collected semen was immediately transported to laboratory and evaluated for different macroscopic parameter (color, volume and thickness). Fresh semen was then diluted with saline solution and evaluated for sperm concentration, motility, sperm kinematics and morphology. Semen samples that fill all the standard were selected for freezing and diluted with Tris-egg yolk citrate diluter. Diluted semen was equilibrated, cryopreserved and finally evaluated for post thaw sperm quality. Different motility parameter (total, progressive, static and slow motility) varied significantly (p<0.01) irrespective of different freezing stages. Significantly higher progressive sperm motility and viability of buffalo spermatozoa were observed at fresh semen whereas lower progressive sperm motility and viability was found at post thaw stage. Total and progressive motility reduced by 2.5 and 2.12% following equilibration, whereas following cryopreservation, total and progressive motility reduced by 35.7 and 28.51% and static motility increases accordingly (35.4%). Significantly higher plasma membrane integrity of sperm was observed at fresh semen followed by pre freeze and post thaw semen. Following freezing, integrity of plasma membrane reduces at the rate of 10.81% and 26.7% at pre freezing and post thaw stages. Significantly higher average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), straightness (STR), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) were found for fresh semen followed by pre-freeze and post-thaw semen. Frozen buffalo semen with higher progressive motility and motion characteristics may be produced if motility losses can be reduced during freezing stage as this stage results higher motility losses. Asian Australas. J. Biosci. Biotechnol. 2022, 7(2), 75-81

Climate change and natural disasters have unequivocal impacts on global agricultural production systems thereby its threats hampering food production and security. The current study was undertaken to identify the natural disasters affecting cattle farming along with the strength of the ability of the farmers to tackle cattle against natural disaster and knowledge and feelings on the impact of climate change in the Bangladesh perspective. A total of 719 cattle rearing households were surveyed from selected 14 upazilas of 9 districts. Data were analyzed with SPSS 20.0 software using descriptive statistics. From the study, it was found that most of the households (95.13%) were farm households depending on agricultural activities. The average herd sizes of indigenous, Red Chittagong Cattle (RCC) and crossbred cattle were 3.84, 2.67, and 7.64 respectively. The average number of bull calf/bull in the case of indigenous (2.22) and RCC (2.43) were higher indicating that farmers were mainly reared these genotypes of cattle for fattening purpose and in the case of crossbred the average number of the milch cow was higher (3.04) indicating farmers rearing crossbred cattle mainly for dairy purpose. However, irrespective of areas 52.97% of cattle rearing farmers thought that natural disaster affected their cattle rearing and the highest 62.08% of farmers thought that the cattle rearing affected by flood followed by cyclone (57.83%), heavy rainfall (54.00%), thunderstorm (44.79%), drought (24.07%) and river erosion (9.66%). Moreover, overall 43.71% of cattle farmers and the highest 88.89% cattle farmers in Sylhet district reported that they were unable to tackle cattle against natural disasters. Furthermore, overall 12.95% of cattle farmers found they were ignorant about the climate change issues. The farmers in Bandarban (100.00%), Tangail (66.67%), Jessore (94.87%), and Rajshahi (76.19%) districts were had knowledge and feeling, on the other hand in Kurigram (97.83%) and Mymensingh (45.45%) districts farmers had no knowledge and feeling over climate change issues. Cattle farmers assumed that cattle health (64.22%) and production performance (49.09%) were mostly affected by climate change issues. Climate change effect and increasing weather extremes, directly and indirectly affects cattle rearing, thus impose bigger consequences to the livestock economy, alongside national food security. There has been no direct study conducted on the effects of climate change on cattle rearing in Bangladesh. This study affords to explore the effects of climate change and natural disasters on cattle rearing and provides preliminary guidelines to policymakers and for further research in the Bangladesh perspective.