Detection of group 2 Dermatophagoides pteronyssinus allergen for environmental monitoring of dust mite infestation

Liao, En-Chih; Lin, Yi-Hsueh; Tsai, Jaw‐Ji

doi:10.5582/bst.2013.v7.2.82

Cited by 8 publications

(4 citation statements)

References 19 publications

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“…The group 2 measurement reflects mite body proteins and is more heat-stable than the Der p 1 allergen associated with a faecal protein (11), and Der p 2 has been shown to correlate with mite numbers indoors (17). However, we do not know whether the vehicle results reflect the transfer of the living mites from home or only the transfer of the allergenic protein.…”

Section: Resultsmentioning

confidence: 55%

Levels of house dust mite allergen in cars / Razine alergena prašinskih grinja u automobilima

Mason

Smith

Anua

et al. 2015

Archives of Industrial Hygiene and Toxicology

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This small study investigated house dust mite (HDM) allergen levels in cars and their owners' homes in north-east Scotland. Dust samples from twelve households and cars were collected in a standardised manner. The dust samples were extracted and measured for the Dermatophagoides group 2 allergens (Der p 2 and Der f 2) and total soluble protein. Allergen levels at homes tended to be higher than in the cars, but not significantly. However, they significantly correlated with paired car dust samples expressed either per unit weight of dust or soluble protein (rho=0.657; p=0. 02 and 0.769; p=0.003, respectively). This points to house-to-car allergen transfer, with the car allergen levels largely reflecting levels in the owner's home. Car HDM allergen levels were lower than those reported in Brazil and the USA. Twenty-five percent of the houses and none of the cars had allergen levels in dust greater than 2000 ng g -1 . This value is often quoted as a threshold for the risk of sensitisation, although a number of studies report increased risk of sensitisation at lower levels. This small study does not allow for characterisation of the distribution of HDM allergen in vehicles in this geographic area, or of the likely levels in other warmer and more humid areas of the UK. Cars and other vehicles are an under-investigated micro-environment for exposure to allergenic material.

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Section: Resultsmentioning

confidence: 55%

Levels of house dust mite allergen in cars / Razine alergena prašinskih grinja u automobilima

Mason

Smith

Anua

et al. 2015

Archives of Industrial Hygiene and Toxicology

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“…Typically, the most used technique for this type of allergen quantification is an enzymatic immunoassay based on IgG monoclonal antibodies (Platts-Mills, et al, 1997;Mistrello, et al, 1992). Although the ELISA test using IgG antibodies has shown good sensitivity and specificity (Liao, et al, 2013;Chapman, et al, 2001), the use of these type of antibodies has great limitations, among them, the high production cost of the assay, which limits its use in the routine indoor, workplace, and public sites monitoring and control of mite biological contamination. Developing alternative efficient, sensitive, and reproducible low-cost methods would be highly advantageous for the primary prevention of indoor mite contamination.…”

Section: Discussionmentioning

confidence: 99%

Immunological detection in indoor environments of house dust mite allergens using avian antibodies - IgY: An innovative tool

Meza

Egea

Garavito

et al. 2021

Rev. Acad. Colomb. Cienc. Ex. Fis. Nat.

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IgY technology has emerged as an alternative for antibody production with immunodiagnostic purposes. Recently, we produced avian antibodies (IgY) against group-1 dust mite allergens of Dermatophagoides sp (Dsp) and Blomia tropicalis (Bt). These allergens are important sensitizing agents that contribute to the pathophysiology of allergy rhinitis and asthma in patients living in tropical areas. The purpose was to explore the utility of IgY for detecting dust mite allergens in indoor environments. We collected 100 samples of dust from mattresses of asthmatic individuals living in two cities on the Colombian Caribbean coast. We used an in-home indirect enzyme-linked immunosorbent assay (ELISA) to detect the mite allergens using two IgYs (IgY-PO2 and IgY-PO4). We determined the IgY detection limit using whole body extracts of Dsp and Bt; likewise, IgY specificity was probed against one crude extract of cockroach, as well as the precision and accuracy of measurements. Both antibodies recognized allergens in the mite extracts, even at the lowest tested concentration (0.05 µg/mL); none reacted with the cockroach extract demonstrating their specificity for mite allergens. On the other hand, IgY-PO4 showed the best precision (intra-assay: CV < 6.99%; inter-assay: CV ≤ 8.71%) and an accuracy: 80.62 – 100.39%. Allergen levels detected were consistent with the mite density present in the dust samples. We confirmed the usefulness of IgY-PO2 and IgYPO4 for detecting dust mite pollution in indoor environments.

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“…This is can be contributed to more than 80% of the dust samples contained house dust mites including D. pteronyssinus and D. farinae in Taiwan [ 56 ]. Most of these allergic patients were exposed to them by contact with mattresses or carpets in the household environment then sensitized to house dust mites of D. pteronyssinus and D. farinae [ 57 ]. Because of their abundance and high allergenicity of D. pteronyssinus and D. farinae , the major predominant mites that elicit positive IgE responses and provoke airway hypersensitivity in Taiwan [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Identification the Cross-Reactive or Species-Specific Allergens of Tyrophagus putrescentiae and Development Molecular Diagnostic Kits for Allergic Diseases

Tsai

Lin

et al. 2020

Diagnostics

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Mite allergens are considerable factors in the genesis of allergic diseases. The storage mite Tyrophagus putrescentiae (Tp) appears in contaminated foods and household surroundings. The current diagnostic tools for Tp allergy are mostly based on crude extracts and still contain shortcomings. This study aimed to investigate the immunoglobulin E (IgE)- responsiveness profiles of Tp-allergic patients and develop a molecular diagnostic method using recombinant allergens. Allergenic components were characterized as cross-reacting or species-specific allergens, in which the effective combinations of recombinant allergens were developed and analyzed in terms of the prediction accuracy for clinical diagnosis. Seven recombinant allergens were cloned and generated to detect the IgE responsiveness of the Tp allergy. A survey on the prevalence of mite allergy showed there were higher sensitizations with IgE responsiveness to house dust mites (HDM) (78.9–80.9%) than to storage mites Tp (35.6%). Prevalence of sensitization to Tp was higher in elderly subjects. The principal IgE-binding components of Tp were Tyr p 1, Tyr p 2 and Tyr p 3. Prediction accuracy for Tp allergy by IgE-responsiveness combination D (Tyr p 1, Tyr p 2 & Tyr p 3) was with high precision (100%). Avoiding the cross-reactivity of Dermatophagoides pteronyssinus, the prediction accuracy of IgE-responsiveness combination H+ (Tyr p 1, Tyr p 2, Tyr p 3, Tyr p 7, Tyr p 8, Tyr p 10 & Tyr p 20) was suitable for Tp-specific diagnosis. Panels of Tp allergens were generated and developed a diagnostic kit able beneficial to identify IgE-mediated Tp hypersensitivity.

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Detection of group 2 Dermatophagoides pteronyssinus allergen for environmental monitoring of dust mite infestation

Cited by 8 publications

References 19 publications

Levels of house dust mite allergen in cars / Razine alergena prašinskih grinja u automobilima

Levels of house dust mite allergen in cars / Razine alergena prašinskih grinja u automobilima

Immunological detection in indoor environments of house dust mite allergens using avian antibodies - IgY: An innovative tool

Identification the Cross-Reactive or Species-Specific Allergens of Tyrophagus putrescentiae and Development Molecular Diagnostic Kits for Allergic Diseases

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