Detection of Group A Streptococci in Throat Cultures by Immunofluorescence: Development and Application of a Specific Reagent

Redys, John J.; Parzick, Arline B.; Borman, Earle K.

doi:10.2307/4591759

Cited by 15 publications

(4 citation statements)

References 6 publications

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“…These organisms contain antigenic components closely related to group A. The cross-reactions in the FA technique were eliminated by the adsorption of the fluorescein-'lPresented in part at the 66th Annual Meeting of the American Society for Microbiology, Los Angeles, Calif., 1-5 May 1966. conjugated group A antiserum with group C cells, followed by the addition of a small amount of unconjugated group C antiserum to the fluorescein-conjugated group A antiserum (15).…”

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Differentiation of Cariogenic Streptococci by Fluorescent Antibody

Jablon

Zinner

1966

J Bacteriol

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Eight strains of streptococci were isolated from human carious lesions by the fluorescent-antibody (FA) technique. Seven of these strains produced experimental caries in hamsters or rats maintained on a high sucrose diet. The eighth strain was noncariogenic in animals but possessed some antigenic components in common with the cariogenic strains. On the basis of antigen-antibody reactions by microprecipitin and agar-gel diffusion patterns, the strains were divided into four groups; these groups differed with regard to their cariogenic activity in hamsters. Fluorescein-conjugated antisera, prepared against the human strains, showed some cross-reactions which interfered with the efficacy of the FA technique in differentiating between the related streptococcal groups. To eliminate these cross-reactions, a small amount of related-strain antisera was added to the fluorescein-conjugated antisera to the cariogenic strains. This technique is effective in blocking crossreactions and should be tried wherever cross-reactions are encountered in the FA technique.

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Differentiation of Cariogenic Streptococci by Fluorescent Antibody

Jablon

Zinner

1966

J Bacteriol

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“…Since that time, several investigators have called attention to the frequent occurrence in fluorescein-conjugated animal sera of antibodies directed against staphylococci (8,9,59,60,63,64). Control of this factor has been an important aspect of the specificity of the FA procedure for grouping of streptococci.…”

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confidence: 99%

Fluorescent-Antibody Techniques in Diagnostic Bacteriology1

Cherry¹,

Moody²

1965

Bacteriological Reviews

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“…When traditional techniques for the detection of streptococci are used, at least 18 h elapse between the receipt of a specimen and the issuance of a culture report; however, there are some circumstances such as major burns for which the early detection of Streptococcus pyogenes may be important. Various methods for the earlier detection of S. pyogenes and other streptococci have been described, including short incubation of swabs in selective enrichment broths followed by fluorescent-antibody staining (1,9), staphylococcal coagglutination (10,11), latex agglutination (7,9), or counterimmunoelectrophoresis (4) to detect streptococcal antigens in the broth. Methods involving the direct examination of the specimen include the use of fluorescentantibody staining smears (6, 8), the extraction of throat scrapings with nitrous acid and precipitation detection of antigen (5), and the detection of streptococcal antigen in throat gargles with latex agglutination (3).…”

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confidence: 99%