Detection of HCV by PCR in serum and PBMC of patients with hepatitis C after treatment

Cavalheiro, Norma de Paula; Filgueiras, Thelma Cristina; Melo, Carlos Eduardo de; Morimitsu, Suzana Rie; Araújo, Evaldo Stanislau Affonso de; Tengan, Fátima Mitiko; Barone, Antonio

doi:10.1590/s1413-86702007000500006

Cited by 20 publications

(23 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The 100% sensitivity and 96.7% specificity found for the PBMCs PCR validated this test as a potential diagnostic tool for HCV infection. These results are in full agreement with previous reports 15. The 3.3% of negative subjects who tested positive by PBMCs PCR might be considered cryptogenic OCI as previously reported by other investigators 17–19.…”

Section: Discussionsupporting

confidence: 93%

See 1 more Smart Citation

Hepatitis C Virus RNA Strands Detection in Peripheral Blood Mononuclear Cells Legitimizes Virus Eradication in Negative Serum PCR Naïve and Post-treatment Patients

Alla¹,

Awady²

2017

Journal of Clinical and Translational Hepatology

View full text Add to dashboard Cite

Background and Aims: Hepatitis C virus (HCV) hepatotropism is associated with intra-peripheral blood mononuclear cell (PBMC) infection that causes post-treatment relapse in RNA seronegative patients. Our understanding of the association of non-viremic hepatic fibrosis with positive anti-HCV IgG antibodies and active hepatocellular damage might be increased by PBMCs screening for intracellular infection. Thus, the goals of this study included evaluation of PBMCs PCR for diagnosing HCV infection, addressing PBMCs plus serum real-time (SRT) PCR benefits over SRT-PCR alone, studying intra-PBMCs distribution of RNA sense and antisense strands, and identifying treatment feasibility in solitary intracellular infection.Methods: Enzyme-linked immunosorbent assay, SRT-PCR and PBMCs PCR were used to evaluate HCV infection in 401 subjects. The patients were classified into groups of negative controls (n = 30), positive controls (n = 63), non-viremia post-treatment (experienced; n = 166) and naïve (n = 49) cases, and non-viremia positive PBMCs PCR naïve (n = 35) and experienced (n = 58) patients.Results: The diagnosis of true positive and negative by PBMCs PCR and SRT-PCR had 100% and 96.7% compatibility respectively. PBMCs PCR detected intracellular HCV infection in 49 out of 215 non-viremia patients; among them, naïve cirrhotics had significantly higher number of intracellular infection than the naïve non-cirrhotic (p < 0.001) and experienced patients (p < 0.0001). Antisense and sense strands were respectively recognized in naïve and experienced cases (p = 0.01218). Intracellular HCV strands were detected in 18.02% of experienced patients. Recognition of intracellular RNA strands showed significant decline in experienced compared to naïve patients (p < 0.05).Conclusion: PBMCs PCR is a valid diagnostic test that can diagnose intracellular HCV when SRT-PCR is negative. Antisense and sense strands are respectively recognized more often in naïve and experienced patients. The expected overall relapsing rate in our cohort was 18.02%. Intra-PBMC infections are associated with liver cirrhosis in naïve non-viremia patients. Eradication of intracellular strands is recommended to avoid RNA seroconversion.Ethical approval certificate: Registration number 10231.

show abstract

Section: Discussionsupporting

confidence: 93%

“…All patients with positive HCV SRT-PCR are also positive on PBMCs PCR. However, some patients with positive PBMCs PCR may have negative SRT-PCR 15. Follow-up of treatment-experienced HCV patients who presented with negative SRT-PCR and positive intra-PBMCs strand infection revealed HCV RNA seroconversion within 1 year 2.…”

Section: Introductionmentioning

confidence: 99%

Hepatitis C Virus RNA Strands Detection in Peripheral Blood Mononuclear Cells Legitimizes Virus Eradication in Negative Serum PCR Naïve and Post-treatment Patients

Alla¹,

Awady²

2017

Journal of Clinical and Translational Hepatology

View full text Add to dashboard Cite

show abstract

“…In fact, extrahepatic reservoirs have important implications for transmission, disease progression, and effective treatment of HCV-infected patients. Clearance of HCV following antiviral treatment does not mean eradication of the virus from whole reservoirs (22). The presence of HCV RNA in PBMCs may lead to HCV reactivation in patients with SVR under special circumstances, such as immunosuppression (23).…”

Section: Discussionmentioning

confidence: 99%

The Role of Polymorphisms Near IFNL3 Gene as Predictors of Residual HCV RNA in Buffy Coat after Successful Antiviral Therapy

et al. 2017

View full text Add to dashboard Cite

Background and Aims: The presence of the hepatitis C virus (HCV) in cells of extrahepatic organs like peripheral blood mononuclear cells (PBMCs) have important implications for transmission, disease progression, and effective treatment of HCV-infected patients. The impact of host genetics such as polymorphisms near Interferon lambda 3 (IFNL3) on clearance of HCV RNA from buffy coat (BC) following successful clearance of HCV from plasma using Pegylated-IFN (PegIFN) and Ribavirin (RBV) treatment was evaluated in our study. Methods: For detection of residual HCV RNA in BC samples, blood samples of 69 patients with sustained virologic response (SVR) after treatment with PegIFN and RBV were evaluated. Polymorphisms near IFNL3 gene including rs12979860 and rs8099917 were assessed using PCR-RFLP method. Results: The most prevalent rs12979860 and rs8099917 genotypes were CT (49.3%) and TT (62.3%), respectively. Nine (13.04%, 95%CI: 7.01% -22.96%) patients had HCV RNA in their BC samples. The favorable genotypes of the 2 polymorphisms (rs12979860 CC and rs8099917 TT) were more frequently observed in patients with undetectable HCV RNA in their BC samples than those with HCV RNA in their BC samples (rs12979860 CC, 45% vs. 22.2%, P = 0.016 and rs8099917 TT, 66.7% vs. 33.3%, P = 0.01). Conclusions:The polymorphisms of IFNL3 could play a crucial role not only in spontaneous clearance of HCV and SVR rate after PegIFN and RBV therapy, but also in the clearance of HCV from BC after PegIFN and RBV therapy.

show abstract

“…The first report about HCV was reported in 1992 by screening 45 patients from the northern part of Pakistan [9]. There are six major genotypes of HCV and more than one hundred subtypes reported throughout the world [10]. Out of these, the genotype-1, genotype-2 and genotype-3 are most popular genotypes in terms of prevalence [10].…”

Section: Introductionmentioning

confidence: 99%

“…There are six major genotypes of HCV and more than one hundred subtypes reported throughout the world [10]. Out of these, the genotype-1, genotype-2 and genotype-3 are most popular genotypes in terms of prevalence [10]. The prevalence rate varies from region to region and have variation geographically.…”

Section: Introductionmentioning

confidence: 99%

Molecular prevalence of hepatitis C virus genotypes in district Kohat, Khyber Pakhtunkhwa, Pakistan

Ullah

Ali

et al. 2017

PAB

View full text Add to dashboard Cite

Hepatitis C Virus is a causative agents of liver cirrhosis, having highly diversified genome. Genotype study has important role in clinical setup and prognosis of hepatitis C virus infection. The aim of this was to investigate the prevalence and risk factors associated with hepatitis C virus genotypes in district Kohat, Khyber Pakhtunkhwa, Pakistan. Hepatitis C virus pre-infected patients (n = 600) were analyzed to assess circulating HCV genotypes, using reverse transcriptase approach. Moreover serum ALT levels were determined followed by correlation with genotypes. In current study cohort, majority of patients (62%) were, having elevated ALT (> 50 U/L). similarly patients with mixed HCV genotypes, 3a/3b have relatively higher ALT levels (~62) compared to 2a/3a (~43) and others. We found significant correlation (p = 0.002) of genotype 2a in connection with ALT (49.48) level. Similarly 3a, 3b, mixed as well as untypeable genotypes correlations were also found highly significant (p <0.001) with their mean ALT levels (47.14, 31.58, 46.69 and 43.68) respectively. In our studied population, most prevalent genotypes were 3a (25%) followed by 3b (20%) and 2a (15%). Fifteen percent of patient's infections were untypeable while in 10% patients mixed genotype were observed. Among total 30 (5%) were blood transfusion cases, 90(15%) surgical, 540 (90%) dentistry, 360 (60%) Barber, 492 (82%) Pricks, whereas 180 (30%) cases were those having early type of HCV/HBV infection in their family. In current study, genotypes 3a and 3b were more prevalent, with two potential risk factors; dentistry and barbers. Moreover, genotypes and ALT investigations were found to be more fruitful in prognosis as well as management of HCV infection.

show abstract

Detection of HCV by PCR in serum and PBMC of patients with hepatitis C after treatment

Cited by 20 publications

References 23 publications

Hepatitis C Virus RNA Strands Detection in Peripheral Blood Mononuclear Cells Legitimizes Virus Eradication in Negative Serum PCR Naïve and Post-treatment Patients

Hepatitis C Virus RNA Strands Detection in Peripheral Blood Mononuclear Cells Legitimizes Virus Eradication in Negative Serum PCR Naïve and Post-treatment Patients

The Role of Polymorphisms Near IFNL3 Gene as Predictors of Residual HCV RNA in Buffy Coat after Successful Antiviral Therapy

Molecular prevalence of hepatitis C virus genotypes in district Kohat, Khyber Pakhtunkhwa, Pakistan

Contact Info

Product

Resources

About