Detection of Helicobacter pylori specific DNA in human atheromatous coronary arteries and its association to prior myocardial infarction and unstable angina

Kowalski, Marek; Rees, W.; Konturek, Peter Christopher; Grove, R.; Scheffold, Thomas; Meixner, H; Brunec, M.; Franz, Norbert; Konturek, J. W.; Pienia̦żek, Piotr; Hahn, Eckhart G.; Konturek, S J; Thale, J.; Warnecke, H.

doi:10.1016/s1590-8658(02)80036-6

Cited by 51 publications

(39 citation statements)

References 27 publications

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“…Other examples of inconsistent NAAT-based associations are atherosclerosis linked to Helicobacter pylori (22), herpes simplex virus, cytomegalovirus and Epstein-Barr virus (14,21), and periodontal microorganisms (6,10,11). A number of "syndromes of yet unknown etiology," including chronic fatigue syndrome, fibromyalgia, and sarcoidosis, have been also linked to Mycoplasma (9, 26), Borna disease virus (10, 31), HHV-8 (4, 7, 12), and Propionibacterium spp.…”

Section: Wanted: Etiologic Agentsmentioning

confidence: 99%

In-House Nucleic Acid Amplification Assays in Research: How Much Quality Control Is Needed before One Can Rely upon the Results?

2005

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3Over the last 20 years, nucleic acid amplification tests (NAATs) have become a major tool for detection of microorganisms, for diagnostic testing, and for research purposes in the field of infectious diseases. NAATs offer significant sensitivity and speed compared to culture and do not require viable organisms. However, validated, commercially available, U.S. Food and Drug Administration-cleared assays exist for the following microorganisms: Mycobacterium tuberculosis, Chlamydia trachomatis, Neisseria gonorrhoeae, methicillin-resistant Staphylococcus aureus, group B streptococcus, Legionella pneumophila, human immunodeficiency virus, hepatitis B virus, and hepatitis C virus. Some of these tests are for very limited indications, for example, the methicillin-resistant Staphylococcus aureus assay is intended only for use with nasopharyngeal swabs as an infection control tool.There are also a number of so-called analyte-specific reagents commercially available for clinically relevant pathogens and pathogenicity factors like herpes simplex virus, EpsteinBarr virus, cytomegalovirus, Streptococcus pyogenes, and Bordetella pertussis and the genes for vanA/vanB and mecA, respectively. Next to these relatively closed and standardized kit concepts, the use of NAATs for research purposes has expanded dramatically. These assays range from those that are well validated to not validated at all, yet these assays are frequently used and cited in the literature. A review of the current literature on the association of a particular microorganism and a particular disease frequently reveals inconsistent results, even apparently when the same methods are used.Although NAATs offer the promise of exquisite sensitivity, theoretically allowing for detection of a single organism in a clinical sample, both false-negative and -positive results can and do occur. There can be problems with sensitivity, specificity, and contamination, which can be secondary to a very large number of technical issues, as listed in Tables 1 to 6. Some of the more common problems in context with NAAT-based studies of microorganisms and disease associations are described below. FALSE-POSITIVE RESULTS DUE TO CONTAMINATIONThe possible association between human herpesvirus 8 (HHV-8) and a direct role in Kaposi's sarcoma pathogenesis is very important. The presence of this potentially oncogenic virus in semen donated for use in artificial insemination would be critically important to know. However, the prevalences of HHV-8 DNA in prostate and semen as measured by NAATs range from 0 to over 90% (32). A recent study (32) assessed interlaboratory sensitivity and reproducibility in the analysis of blinded, identical experimental panels, each consisting of 48 samples composed of semen specimens from artificial insemination donors, human immunodeficiency virus-infected patients, and positive and negative controls. As no commercial, validated assay is available, each laboratory followed its standard procedures for HHV-8 PCR; thus, procedures varied from laboratory to labora...

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Section: Wanted: Etiologic Agentsmentioning

confidence: 99%

In-House Nucleic Acid Amplification Assays in Research: How Much Quality Control Is Needed before One Can Rely upon the Results?

2005

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“…After Mendall et al 25) first demonstrated an increased prevalence of H. pylori infection in patients with documented coronary heart disease compared with that observed in healthy controls, many other researchers investigated this association, and additional supporting data have emerged from groups in England 26) , Croatia 27) , Turkey 28) and Iran 29) . H. pylori, which was previously thought to be restricted to the gastric mucosa, has since been found in human atherosclerotic plaques on polymerase chain reaction and immunohistochemistry 13,30,31) . This finding provides perhaps the most persuasive evidence supporting the direct involvement of this bacterium in pathologies underlying plaque modification.…”

Section: Introductionmentioning

confidence: 99%

Helicobacter pylori—An Infectious Risk Factor for Atherosclerosis?

Yang

Lü

2014

JAT

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Accumulating evidence implicates Helicobacter pylori (H. pylori) infection in the pathogenesis of certain diseases localized outside the stomach, particularly those characterized by persistent and lowgrade systematic inflammation. Recently, the role of H. pylori infection in the development of atherosclerosis and its clinical complications has received attention. Atherosclerosis is a high-cost disease, and acute events resulting from this condition rank first among morbidity and mortality statistics in most industrialized countries. Atherosclerosis is a multifactorial disorder, and traditional risk factors explain only 50% of its etiology. Therefore, identifying new risk factors for atherosclerosis is necessary. Serological studies indicate that chronic H. pylori infection, especially that with more virulent strains, may predispose patients to the onset of atherosclerosis and related adverse clinical events, and PCR studies have detected H. pylori DNA in atherosclerotic plaques, although this finding remains controversial. If this association were to be confirmed, its importance to public health would be substantial, as the eradication of H. pylori is more straightforward and less costly than the longterm treatment of other risk factors. This review investigates the potential relationship between H. pylori infection and atherosclerosis from both epidemiological and pathogenic perspectives and characterizes the potential mechanisms underlying this correlation.

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“…Several studies support pathogenic link between H. pylori infection and coronary atherosclerosis. H. pylori is probably directly involved in pathogenesis of coronary heart disease because the DNA of H. pylori has been found in coronary arteries of individuals with coronary artery disease 11 . Infectious agents like H. pylori are likely involved not only in the initiation of the atherosclerosis, but also in the progression of the atherosclerosis as well.…”

Section: Discussionmentioning

confidence: 99%

Early prognosis of unstable angina patients with positive Helicobacter pylori IgG values

Kaulgud

Kamath

2013

Int Jour of Biomed Res

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Detection of Helicobacter pylori specific DNA in human atheromatous coronary arteries and its association to prior myocardial infarction and unstable angina

Cited by 51 publications

References 27 publications

In-House Nucleic Acid Amplification Assays in Research: How Much Quality Control Is Needed before One Can Rely upon the Results?

In-House Nucleic Acid Amplification Assays in Research: How Much Quality Control Is Needed before One Can Rely upon the Results?

Helicobacter pylori—An Infectious Risk Factor for Atherosclerosis?

Early prognosis of unstable angina patients with positive Helicobacter pylori IgG values

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