Detection of hepatitis A viral RNA in sera of patients with acute hepatitis A

Kwon, Oh Sang; Byun, Kwan Soo; Yeon, Jong Eun; Park, Sang Hoon; Kim, Jae Seon; Kim, Ju Hyun; Bak, Young Tae; Kim, Jin Ho; Lee, Chang Hong

doi:10.1046/j.1440-1746.2000.02291.x

Cited by 29 publications

(22 citation statements)

References 21 publications

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“…This result differs from those of other studies, which show greater molecular marker positivity from anti-HAV-IgM positive samples (Fujiwara et al 1997, Kwon et al 2000, de Paula et al 2002, 2004a, b, Villar et al 2004, Poovorawan et al 2005. This difference could be explained by the fact that the serum samples were stored for a long time and for this reason, the viral RNA could have been degraded.…”

Section: Discussioncontrasting

confidence: 63%

“…This difference could be explained by the fact that the serum samples were stored for a long time and for this reason, the viral RNA could have been degraded. Also, the primers utilized were different from those used in some studies (Fujiwara et al 1997, Kwon et al 2000, Poovorawan et al 2005. In any case, these results confirm the HAV circulation in Goiânia, underscore the importance of HAV RNA detection in the elucidation of acute hepatitis cases of unknown etiology, and suggest the need to adopt this methodology in blood banks.…”

Section: Discussionmentioning

confidence: 50%

See 1 more Smart Citation

Prevalence of hepatitis A virus infection in Goiânia, Goiás, Brazil, by molecular and serological procedures, 1995-2002

Fiaccadori¹,

Soares

Borges³

et al. 2006

Mem. Inst. Oswaldo Cruz

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Section: Discussioncontrasting

confidence: 63%

Section: Discussionmentioning

confidence: 50%

Prevalence of hepatitis A virus infection in Goiânia, Goiás, Brazil, by molecular and serological procedures, 1995-2002

Fiaccadori¹,

Soares

Borges³

et al. 2006

Mem. Inst. Oswaldo Cruz

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“…Other studies report viraemic periods varying from 22 up to 490 days [Bower et al, 2000;Kwon et al, 2000;Ida et al, 2002;Costa-Mattioli et al, 2002b;Sagnelli et al, 2003;Normann et al, 2004].…”

Section: Discussionmentioning

confidence: 99%

High and persistent excretion of hepatitis A virus in immunocompetent patients

et al. 2006

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The duration and level of virus excretion in blood and faeces of patients with hepatitis A virus (HAV) infection were studied in relation to levels of alanine aminotransferase (ALT), disease severity and HAV genotype. Clinical data, blood and faeces were collected from 27 patients with acute hepatitis A (median age: 33 years) for a maximum of 26 weeks. Single blood donations from 55 other patients with acute HAV (median age: 32 years) were also used. Virus loads were quantified by competitive nested RT-PCR. HAV was excreted in faeces for a median period of 81 days after disease onset, with 50% of patients still excreting high levels at Day 36 (2 Â 10 6 -2 Â 10 8 copies/ml faeces suspension). Viraemia was detected, but not quantifiable, for a median period of 42 days. In the first 10 days of illness, higher ALT levels were correlated with higher viraemia levels. Comparison of patients infected with genotype 1a with those infected with type 1b did not differ significantly in terms of the duration of HAV excretion or jaundice. In conclusion, faecal excretion of HAV is at a high titre in the first month, perhaps making patients infectious for a longer period than assumed currently. Blood banks should be aware that viraemia may be present for more than 1 month, and genotype did not affect the duration of virus excretion or jaundice.

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“…Hepatitis A virus is one of the most common causative agents for acute hepatitis worldwide, which accounts for approximately one quarter of clinical hepatitis cases (Yotsuyanagi et al, 1996;Kwon et al, 2000;Hussain et al, 2011). Seroprevalence of anti-HAV is highly correlated with socioeconomic status and access to clean water and sanitation (Jacobsen and Koopman, 2004).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of hepatitis A viral RNA and antibodies among Chinese blood donors

Sun

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Like other developing countries, China was reported to have a relatively high seroprevalence of anti-hepatitis A antibodies (anti-HAV). However, no studies have evaluated the prevalence of anti-HAV and HAV RNA among voluntary blood donors with or without elevated serum alanine transaminase (ALT) levels. Anti-HAV antibodies were detected using an enzyme-linked immunosorbent assay, and reverse transcription quantitative polymerase chain reaction was carried out for detection of HAV RNA. In the current study, we analyzed a total of 450 serum samples with elevated ALT levels (≥40 U/L) and 278 serum samples with non-elevated ALT levels. Seroprevalence rates of anti-HAV were 51.6% in donors with elevated ALT and 41.4% in donors with non-elevated ALT; however, none of the samples was positive for HAV RNA. The results of our study showed lower seroprevalence rates of anti-HAV in blood donors (irrespective of ALT levels) than those in published data on Chinese populations. Although donors with elevated ALT had statistically higher prevalence rates of anti-HAV than did those with non-elevated ALT, none of the serum samples had detectable levels of the active virus. In conclusion, our results demonstrate that the transmission of hepatitis A by blood transfusion will occur rarely.

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Detection of hepatitis A viral RNA in sera of patients with acute hepatitis A

Abstract: In conclusion, HAV-RNA RT-PCR is a useful tool to detect HAV viraemia and to study the molecular epidemiology of HAV infection.

Cited by 29 publications

References 21 publications

Prevalence of hepatitis A virus infection in Goiânia, Goiás, Brazil, by molecular and serological procedures, 1995-2002

Prevalence of hepatitis A virus infection in Goiânia, Goiás, Brazil, by molecular and serological procedures, 1995-2002

High and persistent excretion of hepatitis A virus in immunocompetent patients

Prevalence of hepatitis A viral RNA and antibodies among Chinese blood donors

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