Detection of Hepatitis B Virus (HBV) DNA at femtomolar concentrations using a silica nanoparticle-enhanced microcantilever sensor

Hak, Byung; Lee, Sang Myung; Park, Jae Chan; Hwang, Kyo Seon; Kim, Sang Kyung; Lee, Yoon Sik; Ju, Byeong Kwon; Kim, Tae Song

doi:10.1016/j.bios.2009.06.015

Cited by 86 publications

(48 citation statements)

References 23 publications

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“…Бы ло продемонс т рирова но де тек т ирова ние неамплифицированной матричной РНК в общей массе РН К к летк и с пре делом обнару жени я 10 пмоль. С использованием микрокантилевер ного сенсора и усиления с помощью кремниевых наночастиц удалось достичь определения ДНК вируса гепатита В на фемтомолярном уровне [24]. Фрагмент ДНК вируса длиной 243 нук леотида был детектирован с помощью зонда на уровне пикомолей.…”

Section: Eexamples Of Biosensors Based On Microcantilevers For Detectunclassified

“…It was demonstrated detection of not amplified template RNA in the total mass of RNA of the cell with a detec tion limit of 10 pmole. With the use of microcantilever sensor and gain by means of silicon nanoparticles it was succeeded to achieve detection of DNA of the hepatitis b virus at femto molar level [24]. The DNA fragment of the virus with a length of 243 nucleo tide was detected using a probe at the level of picomoles.…”

Section: #5 / 67 / 2016mentioning

confidence: 99%

“…Разработаны способы усиления сигнала, такие как использо вание наночастиц [24] или создание специаль ного матрикса на поверхности кантилевера [22]. Важным лимитирующим фактором для достиже ния максимальной чувствительности является неспецифическое связывание.…”

Section: преимущества и недостатки биосенсоров на основе микрокантилеunclassified

“…Methods of signal ampli fication, such as using nanoparti cles [24] or the creation of a special matrix on the surface of the canti lever [22] are developed. An impor tant limiting factor for maximum sensitivity is non specific binding.…”

Section: Advantages and Disadvantages Of Biosensors Based On Microcanmentioning

confidence: 99%

See 3 more Smart Citations

Cantilever biosensors for detection of viruses and bacteria

Kolesov¹,

Yaminsky²,

Ahmetova³

et al. 2016

NanoRus

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Section: Eexamples Of Biosensors Based On Microcantilevers For Detectunclassified

Section: #5 / 67 / 2016mentioning

confidence: 99%

Section: преимущества и недостатки биосенсоров на основе микрокантилеunclassified

Section: Advantages and Disadvantages Of Biosensors Based On Microcanmentioning

confidence: 99%

See 2 more Smart Citations

Cantilever biosensors for detection of viruses and bacteria

Kolesov¹,

Yaminsky²,

Ahmetova³

et al. 2016

NanoRus

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“…[1][2][3][4][5][6] A piezoresistive microcantilever is a new kind of electrical cantilever, with characteristics of simple read-out way, easy integration, low cost, and small volume. Compared with an optical readout and dynamic-mode cantilever, it is more promising in field detection.…”

Section: Introductionmentioning

confidence: 99%

Piezoresistive microcantilever aptasensor for ricin detection and kinetic analysis

Liu¹,

Tong²,

Li³

et al. 2015

AIP Advances

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Up to now, there has been no report on target molecules detection by a piezoresistive microcantilever aptasensor. In order to evaluate the test performance and investigate the response dynamic characteristics of a piezoresistive microcantilever aptasensor, a novel method for ricin detection and kinetic analysis based on a piezoresistive microcantilever aptasensor was proposed, where ricin aptamer was immobilised on the microcantilever surface by biotin-avidin binding system. Results showed that the detection limit of ricin was 0.04μg L−1 (S/N ≥ 3). A linear relationship between the response voltage and the concentration of ricin in the range of 0.2μg L−1-40μg L−1 was obtained, with the linear regression equation of ΔUe = 0.904C + 5.852 (n = 5, R = 0.991, p < 0.001). The sensor showed no response for abrin, BSA, and could overcome the influence of complex environmental disruptors, indicating high specificity and good selectivity. Recovery and reproducibility in the result of simulated samples (simulated water, soil, and flour sample) determination met the analysis requirements, which was 90.5∼95.5% and 7.85%∼9.39%, respectively. On this basis, a reaction kinetic model based on ligand-receptor binding and the relationship with response voltage was established. The model could well reflect the dynamic response of the sensor. The correlation coefficient (R) was greater than or equal to 0.9456 (p < 0.001). Response voltage (ΔUe) and response time (t0) obtained from the fitting equation on different concentrations of ricin fitted well with the measured values.

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2015

DNA Engineered Noble Metal Nanoparticles

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Detection of Hepatitis B Virus (HBV) DNA at femtomolar concentrations using a silica nanoparticle-enhanced microcantilever sensor

Cited by 86 publications

References 23 publications

Cantilever biosensors for detection of viruses and bacteria

Cantilever biosensors for detection of viruses and bacteria

Piezoresistive microcantilever aptasensor for ricin detection and kinetic analysis

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