Detection of herpes simplex virus DNA in the peripheral blood during acute recurrent herpes labialis

Brice, Sylvia L.; Stockert, Stephen S.; Jester, Joy D.; Huff, J. Clark; Bunker, James D.; Weston, William L.

doi:10.1016/0190-9622(92)70087-v

Cited by 47 publications

(43 citation statements)

References 23 publications

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“…the natural course of the disease is about 2-4 weeks [18]. The fact that erythema multiforme has a definite and acute onset, while vitiligo has an insidious and slow onset may account for the negative detection of HSV in vitiligo serum, as evidenced by study that HSV was no long detected in patients several weeks after clinical resolution [12]. Infection of herpes simplex virus may have already recovered in the long course of vitiligo, and converted to other mechanisms that played the subsequent roles, such as autoimmunity.…”

Section: Discussionmentioning

confidence: 95%

“…The amplification conditions were 94℃ denaturation for 30 seconds, 55℃ annealing for 30 seconds, 72℃ elongation for 1 minute. After 35 cycles of amplification, the PCR products were ran on 2% agarose gel [12][13][14]. Antibodies against herpes simplex virus in serum of patients were detected by chemiluminescence method using Dia Sorin LIAISON @ …”

Section: Methodsmentioning

confidence: 99%

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No Evidence of HSV Presence in the Serum of Vitiligo Patients

Liu¹,

Qi²

2014

Pigmentary Disorders

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Vitiligo is mostly regarded as a consequence of autoimmune response, while the triggering factors remain to be determined. We checked the presence of HSV specific DNA, and IgG and IgM antibodies against HSV, in serum of 90 vitiligo patients by PCR and chemiluminescence methods, respectively. No HSV specific DNA was detected in vitiligo of any types or stages. The seropositivity of antibodies against HSV was similar between the patients and controls. Other approaches should be tried to clarify the role of HSV in the pathogenesis of vitiligo.

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Section: Discussionmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

No Evidence of HSV Presence in the Serum of Vitiligo Patients

Liu¹,

Qi²

2014

Pigmentary Disorders

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“…The emphasis of this report is on the interaction between HIV-1 and HSV-1. One of the difficulties in studying the full range of biological interactions between HIV-1 and HSV-1 is to find an in vitro model that mimicks the environment wherein the two viruses would be likely to come into contact in vivo [Brice et al, 1992]. In the previous study, it was reported that HSV-1 infects peripheral blood lymphocytes (PBLs) activated by mitogenic treatment [Calistri et al, 1999].…”

Section: Introductionmentioning

confidence: 98%

Herpes simplex virus type 1 can either suppress or enhance human immunodeficiency virus type 1 replication in CD4‐positive T lymphocytes

Calistri

Parolin

Palù

2003

Journal of Medical Virology

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It was proposed recently that CEM CD4-positive T cells infected chronically by herpes simplex virus type 1 (HSV-1) and human immunodeficiency virus type 1 (HIV-1) (CEM(HSV/HIV)) may be used as a model for studying HIV/HSV interactions. To ascertain whether HSV-HIV coinfection of T lymphocytes has a role in promoting progression of lentiviral infection, T cells infected chronically by either HSV-1 (CEM(HSV)) or HIV-1 (CEM(HIV)) were challenged with a superinfecting dose of HIV-1 or HSV-1. The results show a positive influence on HIV growth when CEM(HIV) cells were superinfected with HSV-1 to an extent that was dependent on the multiplicity of superinfection used. In contrast, HIV superinfection of CEM(HSV) cells resulted in a delay of HIV-1 production and in a lack of HSV-mediated LTR transactivation. These effects were due to cell growth inhibition and apoptosis, resulting from persistent HSV-1 infection. Treatment of CEM(HSV) with acyclovir inhibited completely the HSV-1 cytopathic effects and allowed efficient HIV-1 replication. These data may be relevant in clarifying the role of HIV/HSV interaction in the pathogenesis of AIDS.

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“…Unfortunately, the diagnosis of HSV hepatitis is often made at autopsy, due to a lack of clinical suspicion. PCR has emerged as a rapid and sensitive diagnostic tool for verification of disseminated HSV disease and should be utilized on plasma, body fluids, and biopsy specimens when possible (2,6).…”

Section: Case Reportmentioning

confidence: 99%