Detection of herpes simplex virus proteins in cultured cells by monoclonal antibodies and the avidin‐biotin‐immunoperoxidase complex method

Hwang, Charles B. C.; Greenspan, John S.; Shillitoe, Edward J.

doi:10.1111/j.1600-0714.1986.tb00602.x

Cited by 9 publications

(1 citation statement)

References 26 publications

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“…. B95-8 cells and Raji cells, both "normal" and TPA-induced, were stained with the same polyclotial rabbit antipapillomavirus serum using the avidinbiotin-peroxidase complex technique described by HWANG et al, (18). To con-firiTi the presence of EBV in the B95-8 cells and the TPA-induced Raji cells, parallel slides were stained with a mouse monoclonal antibody to the EBV early antigen EA-D (MAB 818, Chemicon Inc., Temecula, CA) using murine-specific reagents for the avidin-biotin-peroxidase complex detection.…”

Section: Methodsmentioning

confidence: 99%

Prevalence of Epstein‐Barr virus and human papillomavirus in oral mucosa of HIV‐Infected patients

Adler‐Storthz¹,

Ficarra

Woods

et al. 1992

J Oral Pathology Medicine

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Epstein-Barr virus (EBV) has been implicated in the genesis of oral hairy leukoplakia (OHL). Initially, OHL was also associated with human papillomavirus (HPV) as evidenced by staining with antiserum to papillomavirus common structural antigens and reports of two HPV-positive OHL as detected by in situ DNA hybridization. The aims of this study were to determine the prevalence of EBV and HPV DNA in OHL and normal oral mucosa and to explain the basis for the staining of OHL tissues with antibodies to papillomavirus common structural antigens. EBV DNA was detected by in situ hybridization in 47 of 47 cases of OHL from human immunodeficiency virus (HIV)-seropositive individuals and in 1 of 10 biopsies of clinically normal buccal mucosa from the same group of individuals. Twenty-five of 35 OHL specimens stained with antibody to papillomavirus common structural antigens. There was no staining of two EBV-containing lymphoblastoid lines, indicating that the staining with anti-papillomavirus antibody was not due to antigenic cross-reactivity with EBV antigens. HPV DNA was detected by polymerase chain reaction amplification in 10 of 18 OHL specimens and in 6 of 10 normal buccal mucosa specimens. Our results indicate that EBV and HPV are present frequently in OHL and that HPV can be found regularly in histologically normal mucosa.

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Section: Methodsmentioning

confidence: 99%