Detection of herpes simplex virus DNA sequences in corneal transplant recipients by polymerase chain reaction assays

Cantin, Edouard M.; Chen, Jian; McNeill, James I.; Willey, Dru E.; Openshaw, Harry

doi:10.3109/02713689109020353

Cited by 49 publications

(33 citation statements)

References 18 publications

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“…In different studies this rate was documented as 36% (Cantin et al 1991), and 22% (Kaye et al 2000). In our study we also demonstrated that six out of twenty eight (21%) corneal transplant patient without clinically obvious herpetic keratitis were carrying latent HSV virus in their corneas.…”

Section: Discussionsupporting

confidence: 72%

“…HSV virus can also undergo latency and reactivation in the cornea as an extraneuronal site after HSK disease (Pavan-Langston et al 1989;Rong et al 1991). In previous studies HSV detection rate by PCR ranged from 32% to 82% in donor cornea buttons of subjects with a past history of herpes keratitis (Cantin et al 1991;van Gelderen et al 2000;Kaye et al 2000).…”

Section: Discussionmentioning

confidence: 92%

“…In corneas of clinically unsuspected herpetic keratitis patients HSV DNA was also isolated by PCR (Cantin et al 1991;Kaye et al 2000). In different studies this rate was documented as 36% (Cantin et al 1991), and 22% (Kaye et al 2000).…”

Section: Discussionmentioning

confidence: 99%

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The relationship of graft survival and herpes simplex virus latency in recipient corneal buttons

Aydemir

Türkçüoğlu²,

Bulut³

et al. 2007

OPTH

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Purpose: To demonstrate relationship between herpes simplex virus (HSV) corneal latency and graft survival. Methods: Prospective case control study. 28 recipient corneal buttons and donor cornea-scleral remnants were examined for HSV DNA with polymerase chain reaction (PCR). None of the recipient had a history of HSV infection. Serum samples of graft recipients were analyzed for the presence of anti-HSV IgG and IgM with enzyme-linked immunosorbent assay technique. All corneas were free of stromal scarring or epithelial defect before sampling and had an endothelial cell density of >2000 cells/mm 2 . Results: In twenty three patients (82%) anti-HSV IgG was detected in serum. In none of the recipients anti-HSV IgM was positive. HSV DNA was positive in six out of twenty eight (21%) of the recipient corneal buttons and none of the donor cornea-scleral remnants. In eighteenmonths follow-up period three out of six (50%) HSV DNA positive and one out of twenty-two (4.5%) HSV DNA negative patients experienced late endothelial failure that was statistically signifi cantly different (p = 0.022). Conclusion: Even without a history of HSV keratitis, presence of latent HSV virus in recipient cornea is an important risk factor for subsequent graft survival.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 92%

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The relationship of graft survival and herpes simplex virus latency in recipient corneal buttons

Aydemir

Türkçüoğlu²,

Bulut³

et al. 2007

OPTH

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“…Vários métodos incluindo a cultura viral (14)(15)(16)(17)(18)(19) , reação da cadeia da polimerase (19)(20)(21)(22)(23)(24)(25) , imuno-histoquímica (25)(26) , e hibridização in situ (23) têm sido usados para o diagnóstico do HSV tipo 1 em córneas humanas. O trabalho de El-Aal AM (3) mostrou que a cultura do vírus herpes simples foi positiva em 10 (20,8%) de 48 casos suspeitos de ceratite herpética epitelial dendrítica com infiltrado subendotelial.…”

Section: Discussionunclassified

Detecção do vírus herpes simples por reação em cadeia da polimerase em pacientes com ceratite herpética típica ou atípica

Kamimura

Takata

Fernandes

et al. 2008

Arq. Bras. Oftalmol.

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“…Furthermore, It seems that the prevalence of this virus in the general population of Iran is less than 1% [11], which is lower than the reports of the neighboring countries [12]. For this reason, the current study was designed to report the epidemiologic features of HBV and HCV in Iranian peoples with positive hepatitis screening test [13][14][15][16]. Iran is located in the Middle-East in a position like a bridge between Indian subcontinent, Arab peninsula, Middle Asia, and Europe.…”

mentioning

confidence: 99%

Prevalence and epidemiology of hepatitis C virus (HCV) infection in Rafsanjan

Fatemipour¹,

Fatemipour

Vahedi

et al. 2016

Iran J Virol

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Background and Aims: Many individuals with chronic hepatitis C virus (HCV) infection are asymptomatic, population-based serologic studies are needed to estimate the prevalence of infection which will help to take necessary procedures for prevention and control the disease. This study was conducted to find out the prevalence, of HCV infection among patients referring to the hospital care in Rafsanjan, Iran. Materials and Methods: A total of 940 blood samples (430 males and 510 females) were received and screened for hepatitis C infection during December 2015 to December 2016. After separation of serum from blood samples in local laboratory, all samples were tested for HCV Ag by ELISA tests. Liver enzymes [Alkalin Phosphatase (ALP), Alanine aminotrans¬ferase (ALT) and Aspartate aminotransferase (AST)] were determined using biochemical procedures. Results: Amongst 940 collected samples, 18 (1.91%) were positive for HCV Antibody. Among the positive ones, HCV hepatitis was more prevalent in male patients than the females. The prevalence rate of HCV in male was 3.72% and 0.39% for female. Results related to age showed that higher rate of infection in 20-29 years old (%26.59), and the lowest was found in above 80 years old group (0.31%). Of the three enzymes, only ALP was significantly higher than the control group (P=0.003). Conclusions:The prevalence of HCV in Rafsanjan was at an increasing rate. Findings from the current study will be helpful for better management and control of viral hepatitis C infection.

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Detection of herpes simplex virus DNA sequences in corneal transplant recipients by polymerase chain reaction assays

Cited by 49 publications

References 18 publications

The relationship of graft survival and herpes simplex virus latency in recipient corneal buttons

The relationship of graft survival and herpes simplex virus latency in recipient corneal buttons

Detecção do vírus herpes simples por reação em cadeia da polimerase em pacientes com ceratite herpética típica ou atípica

Prevalence and epidemiology of hepatitis C virus (HCV) infection in Rafsanjan

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