Detection of Heterotrophic Contaminants in Cultures of Thiobacillus ferrooxidans and Their Elimination by Subculturing in Media Containing Copper Sulphate

Johnson, D. Barrie; Kelso, W. I.

doi:10.1099/00221287-129-9-2969

Cited by 28 publications

(24 citation statements)

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“…Considering these features described above, it is apparent that the isolates belong to a species of the genus Acidiphilium. The isolates and Acidiphilium species (6,9,11,18,36) are definitely distinguished from Acidobacterium capsulatum in cellular fatty acid composition, quinone system, G+C content of DNA, and genetic relatedness (13). Acidomonas methanolica (28-30) is similar to our isolates and Acidiphilium species in ubiquinone type and some other respects.…”

Section: Dna Base Composition and Dna-dna Homologymentioning

confidence: 67%

“…and coal mine drainage (20,27), and cultures of Thiobacillus ferrooxidans (5)(6)(7)(8)(9)11,18,36,37). Harrison (6) studied systematically acidophilic, mesophilic, heterotrophic bacteria derived directly from coal strip-mine refuse and also as contaminants in cultures of T. ferrooxidans, and proposed the designation of the genus Acidiphilium for these bacteria.…”

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confidence: 99%

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Acidiphilium multivorum sp. nov., an acidophilic chemoorganotrophic bacterium from pyritic acid mine drainage.

Wakao

Nagasawa

Matsuura

et al. 1994

J. Gen. Appl. Microbiol.

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Section: Dna Base Composition and Dna-dna Homologymentioning

confidence: 67%

mentioning

confidence: 99%

Acidiphilium multivorum sp. nov., an acidophilic chemoorganotrophic bacterium from pyritic acid mine drainage.

Wakao

Nagasawa

Matsuura

et al. 1994

J. Gen. Appl. Microbiol.

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“…Culture-independent rRNA approaches including environmental 16S rDNA cloning and in situ hybridization have demonstrated the occurrence of Acidiphilium species as the major populations in mine drainage (Peccia et al, 2000) and acidic geothermal springs (Burton and Norris, 2000). As several strains of Acidiphilium species were isolated or found as contaminants from cultures of the iron-oxidizing chemolithotroph Acidithiobacillus ferrooxidans (formerly Thiobacillus ferrooxidans, see Kelly and Wood (2000)) (Guay and Silver, 1975;Harrison, , 1989Harrison et al, 1980;Johnson and Kelso, 1983;Lobos et al, 1986), they may aid leaching through the consumption of organic matter inhibitory to the growth and activity of Acidithiobacillus ferrooxidans. Although most Acidiphilium species are obligately heterotrophic, their natural habitats seem to be oligotrophic environments with poor organic matter.…”

Section: Habitats and Ecologymentioning

confidence: 99%

Aerobic anoxygenic photosynthetic bacteria with zinc-bacteriochlorophyll.

Hiraishi

Shimada

2001

J. Gen. Appl. Microbiol.

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IntroductionChlorophylls (Chls) and bacteriochlorophylls (BChls) are porphyrin derivatives chelated with magnesium, and represent a class of lipophilic pigments involved in natural photosynthesis. There has been no rational explanation for why extant phototrophic organisms have used Mg exclusively as the central metal with which the chlorophyllous pigments are complexed. Apart from this question, phototrophic organisms that use Chls or BChls containing metals other than Mg were unknown for a long time. Recently, this common understanding of natural photosynthesis was unexpectedly modified by the new finding that a novel purple pigment, zinc-chelated-BChl (Zn-BChl) (for abbreviations, see Takaichi et al. (1999)), was discovered in a specific group of obligately aerobic bacteria (Hiraishi et al., 1998;Wakao et al., 1996Wakao et al., , 1999.It has been satisfactorily demonstrated that BChls are present not only in facultatively and obligately anaerobic anoxygenic phototrophic bacteria but also in large numbers of species of obligately aerobic bacteria Naturally occurring chlorophyllous pigments, which function as the cofactor in the early photochemical reaction of photosynthesis, have been proven beyond question to be magnesium-complexed porphyrin derivatives. Phototrophic organisms that use (bacterio)chlorophylls ([B]Chls)containing metals other than Mg were unknown for a long time. This common knowledge of natural photosynthesis has recently been modified by the striking finding that a novel purple pigment, zinc-chelated-BChl (Zn-BChl) a, is present as the major and functional pigment in species of the genus Acidiphilium. Acidiphilium species are obligately acidophilic chemoorganotrophic bacteria that grow and produce photopigments only under aerobic conditions. Although the mechanism of photosynthesis with Zn-BChl a in Acidiphilium species is similar to that seen in common purple bacteria, some characteristic photosynthetic features of the acidophilic bacteria are also found. The discovery of natural photosynthesis with Zn-BChl has not only provided a new insight into our understanding of bacterial photosynthesis but also raised some interesting questions to be clarified. The major questions are why the acidophilic bacteria have selected ZnBChl for their photosynthesis and how they synthesize Zn-BChl and express photosynthetic activity with it in their natural habitats. In this article we review the current knowledge of the biology of Acidiphilium as aerobic photosynthetic bacteria with Zn-BChl a and discuss the interesting topics noted above.

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“…It is not clear whether it is the high concentrations of bacteriological agars normally used to form suitable gels or the impurities present within the agars that are inhibitory to A . organovorum and other acidophilic bacteria (10,12).…”

Section: Discussionmentioning

confidence: 99%

“…Without pure-colony isolations, the acquisition of pure cultures of T. ferrooxidans was extremely difficult and often proved unreliable. Frequently, ostensibly pure cultures have contained acidophilic heterotrophs or more than one strain of T. ferrooxiduns (7,10). At least two new species of heterotrophic bacteria have been isolated from T. ferrooxiduns cultures (4, 5,29).…”

mentioning

confidence: 99%

Acidiphilium organovorum sp. nov., an Acidophilic Heterotroph Isolated from a Thiobacillus ferrooxidans Culture

Lobos

Chisolm

Bopp

et al. 1986

International Journal of Systematic Bacteriology

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Detection of Heterotrophic Contaminants in Cultures of Thiobacillus ferrooxidans and Their Elimination by Subculturing in Media Containing Copper Sulphate

Cited by 28 publications

References 0 publications

Acidiphilium multivorum sp. nov., an acidophilic chemoorganotrophic bacterium from pyritic acid mine drainage.

Acidiphilium multivorum sp. nov., an acidophilic chemoorganotrophic bacterium from pyritic acid mine drainage.

Aerobic anoxygenic photosynthetic bacteria with zinc-bacteriochlorophyll.

Acidiphilium organovorum sp. nov., an Acidophilic Heterotroph Isolated from a Thiobacillus ferrooxidans Culture

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