Detection of Heterozygosity Loss in Microdissected Fine Needle Aspiration Specimens of Breast Carcinoma

Chuaqui, Rodrigo F.; Vargas, M. Perla; Castiglioni, Teresa; Elsner, Boris; Zhuang, Zhengping; Emmert-Buck, M. R.; Merino, María J.

doi:10.1159/000333932

Cited by 15 publications

(12 citation statements)

References 23 publications

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“…The analysis of microsatellite regions provides a molecular fingerprint of the tissues examined. LOH has been applied to tumour diagnosis, including largescale analysis of multiple samples, 10 and occasionally in cytological samples, 11,12 but not as yet for the purpose of establishing different tumorigenesis in cytological samples.…”

Section: Discussionmentioning

confidence: 99%

Immunocytochemistry versus molecular fingerprinting of metastases

Salto-Tellez

Zhang

Chiu³

et al. 2003

Cytopathology

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Examination of cytological samples of cancer to suggest a possible primary site of origin is one of the commonest and most difficult tasks of diagnostic cytopathologists. Currently, both cytomorphology and immunocytochemistry are the main approaches to this diagnostic dilemma. We report the application of microsatellite analysis in cytological samples in a patient with a primary colonic tumour and two subsequent lung nodules, which were suspected on CT scans of the chest, and compared the findings with those obtained with conventional immunocytochemistry. The molecular results were in agreement with the radiological impression and conflicted with the immunocytochemistry. We conclude that immunocytochemical and molecular biology approaches to the diagnosis of tumours may give rise to contradictory results.

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Section: Discussionmentioning

confidence: 99%

Immunocytochemistry versus molecular fingerprinting of metastases

Salto-Tellez

Zhang

Chiu³

et al. 2003

Cytopathology

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“…Application of molecular techniques to cell samples is an adjunct to morphology that can be of assistance to the cytologist. Immunohistochemistry, 13,17 flow cytometry, 10,16,19,22 in situ hybridization, 11 fluorescence in situ hybridization 21 and PCR-based methods 4,7 have been applied to cytologic samples. In a recent study the utility of detecting expression of carcinoembryonic antigen by RT-PCR in peritoneal …”

Section: Discussionmentioning

confidence: 99%

Analysis of mRNA Quality in Freshly Prepared and Archival Papanicolaou Samples

Chuaqui

Cole²,

Cuello³

et al. 1999

Acta Cytologica

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“…16 The markers used contain polymorphisms in allelic lengths; thus, the different size alleles can be distinguished on a denaturing polyacrylamide gel based on their mobility. Alleles from patients who are heterozygous, with two alleles of different sizes in normal tissue, will therefore migrate to two separate bands on an electrophoresis gel.…”

Section: Pcr Amplification and Loh Analysismentioning

confidence: 99%

Detection of loss of heterozygosity at chromosome 3p25–26 in primary and metastatic ovarian clear‐cell carcinoma: Utilization of microdissection and polymerase chain reaction in archival tissues

Simsir

Palacios

Linehan

et al. 2001

Diagnostic Cytopathology

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Loss of heterozygosity (LOH) at the 3p region is found in up to 50% of epithelial ovarian neoplasms. The von Hippel-Lindau (VHL) gene at the 3p25 locus is one of the tumor-suppressor genes located at 3p. The role, if any, of the VHL gene locus is not clear in ovarian carcinogenesis. We analyzed primary and metastatic ovarian clear-cell carcinomas (OCCC) for LOH at 3p25 to determine its frequency and its diagnostic utility as an adjunctive tool in the differential diagnosis of metastatic clear-cell carcinomas. Microdissection followed by single-step DNA extraction and polymerase chain reaction (PCR) amplification, using two polymorphic markers flanking the VHL gene locus, was done on archival histology and cytology samples from 9 patients with metastatic OCCC. Of the informative cases, 43% of the metastatic and 50% of the primary OCCC showed LOH. LOH at the VHL gene locus is not uncommon in clear-cell ovarian carcinoma. LOH at 3p25 in cytologic specimens may be a valuable adjunct in the diagnosis of OCCC metastasis in cytologically equivocal cases. OCCC should enter the differential in clear-cell carcinomas of unknown primary that show LOH at 3p25. Published 2001 Wiley-Liss, Inc.

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Detection of Heterozygosity Loss in Microdissected Fine Needle Aspiration Specimens of Breast Carcinoma

Cited by 15 publications

References 23 publications

Immunocytochemistry versus molecular fingerprinting of metastases

Immunocytochemistry versus molecular fingerprinting of metastases

Analysis of mRNA Quality in Freshly Prepared and Archival Papanicolaou Samples

Detection of loss of heterozygosity at chromosome 3p25–26 in primary and metastatic ovarian clear‐cell carcinoma: Utilization of microdissection and polymerase chain reaction in archival tissues

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