Detection of hidden hazelnut protein in food by IgY-based indirect competitive enzyme-immunoassay

Drs, Elisabeth; Baumgartner, Sabine; Bremer, M.G.E.G.; Kemmers-Voncken, A.; Smits, Nathalie G. E.; Haasnoot, W.; Banks, J. N.; Reece, Paul; Danks, C.; Tomkies, Victoria; Immer, Ulrike; Schmitt, Kerstin; Krska, Rudolf

doi:10.1016/j.aca.2004.04.054

Cited by 26 publications

(10 citation statements)

References 10 publications

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“…Goat anti-mouse IgG in PBS (pH 7.6) (1.2 mg/mL; AffiniPure F(ab’) 2 Fragment GAM IgG Fcγ) used for spraying LFIA control lines was purchased from Jackson Immunoresearch Laboratories Inc (Sanbio, Uden, The Netherlands). All other antibodies were developed by RIKILT, Wageningen University & Research (Wageningen, The Netherlands), according to the procedure described in [26,27]. In short, the antibody panel listed in Table 1 was produced by immunizing mice with 50 µg extracted hazelnut (mixed) protein, with booster immunizations containing 25 µg extracted hazelnut protein.…”

Section: Methodsmentioning

confidence: 99%

Rapid Antibody Selection Using Surface Plasmon Resonance for High-Speed and Sensitive Hazelnut Lateral Flow Prototypes

et al. 2018

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Lateral Flow Immunoassays (LFIAs) allow for rapid, low-cost, screening of many biomolecules such as food allergens. Despite being classified as rapid tests, many LFIAs take 10–20 min to complete. For a really high-speed LFIA, it is necessary to assess antibody association kinetics. By using a label-free optical technique such as Surface Plasmon Resonance (SPR), it is possible to screen crude monoclonal antibody (mAb) preparations for their association rates against a target. Herein, we describe an SPR-based method for screening and selecting crude anti-hazelnut antibodies based on their relative association rates, cross reactivity and sandwich pairing capabilities, for subsequent application in a rapid ligand binding assay. Thanks to the SPR selection process, only the fast mAb (F-50-6B12) and the slow (S-50-5H9) mAb needed purification for labelling with carbon nanoparticles to exploit high-speed LFIA prototypes. The kinetics observed in SPR were reflected in LFIA, with the test line appearing within 30 s, almost two times faster when F-50-6B12 was used, compared with S-50-5H9. Additionally, the LFIAs have demonstrated their future applicability to real life samples by detecting hazelnut in the sub-ppm range in a cookie matrix. Finally, these LFIAs not only provide a qualitative result when read visually, but also generate semi-quantitative data when exploiting freely downloadable smartphone apps.

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Section: Methodsmentioning

confidence: 99%

Rapid Antibody Selection Using Surface Plasmon Resonance for High-Speed and Sensitive Hazelnut Lateral Flow Prototypes

et al. 2018

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“…The pollen were obtained from Allergon AB (Ängelholm, Sweden); all other products were obtained from the local market. Extractions were performed as described elsewhere [ 10 ].…”

Section: Methodsmentioning

confidence: 99%

“…The relative responses measured 10 s before the regeneration started were used for calculations. For quantitative analysis, a calibration graph was prepared by spiking extract of pure extra virgin olive oil with aqueous hazelnut protein extracts [ 10 ] to give final hazelnut protein concentrations of 0–5 μg/mL. Extracts of pure hazelnut oils were diluted in extract of pure extra virgin olive oil when needed.…”

Section: Methodsmentioning

confidence: 99%

Biosensor immunoassay for traces of hazelnut protein in olive oil

Bremer¹,

Smits²,

Haasnoot³

2009

Anal Bioanal Chem

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The fraudulent addition of hazelnut oil to more expensive olive oil not only causes economical loss but may also result in problems for allergic individuals as they may inadvertently be exposed to potentially allergenic hazelnut proteins. To improve consumer safety, a rapid and sensitive direct biosensor immunoassay, based on a highly specific monoclonal antibody, was developed to detect the presence of hazelnut proteins in olive oils. The sample preparation was easy (extraction with buffer); the assay time was fast (4.5 min only) and the limit of detection was low (0.08 μg/g of hazelnut proteins in olive oil). Recoveries obtained with an olive oil mixed with different amounts of a hazelnut protein containing hazelnut oil varied between 93% and 109%.Electronic supplementary materialThe online version of this article (doi:10.1007/s00216-009-2720-1) contains supplementary material, which is available to authorized users.

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“…k-Casein from bovine milk (MW approximately 19000 Da), gliadin from wheat, bovine serum albumin (BSA), rabbit polyclonal antigliadin antibody (G9144), goat antimouse IgG antibody, goat antirabbit IgG antibody, goat antimouse IgG antibody Horse Radish peroxidase (HRP) labeled, goat antirabbit IgG antibody HRP labeled, and 3-(aminopropyl)triethoxysilane (APTES) were obtained from Sigma-Aldrich (Munich, Germany). Monoclonal antibodies against bovine k-casein (clones 33−4G10 and 33−6A10), soy protein (clones 26−6C9 and 26−5G4), peanut protein (clones 51−5G9 and 44−4G2) prepared according to a published procedure, 47 as well as soy and peanut protein extracts prepared as reported previously, 48 were provided by RIKILT Wageningen UR (Wageningen, The Netherlands). Microtiter plates were from Greiner Diagnostic GmbH (Bahlingen, Germany).…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Ultrafast Multiplexed-Allergen Detection through Advanced Fluidic Design and Monolithic Interferometric Silicon Chips

et al. 2018

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A silicon-based miniaturized sensor chip combined with an advanced microfluidic module for the simultaneous, label-free immunochemical determination of four allergens, bovine milk protein, peanut protein, soy protein, and gliadin, is presented. The sensor chip consists of an array of 10 broad-band Mach-Zehnder interferometers (BB-MZIs) monolithically integrated on silicon, along with their respective broad-band light sources. The BB-MZIs were biofunctionalized with the targeted allergens and their responses during immunoreaction were monitored by multiplexing their transmission spectra through an external miniaturized spectrometer. The assay is performed by running mixtures of calibrators or samples with the antibodies against the four allergens followed by an antispecies specific antibodies solution. Employing a fluidic module of nearly one-dimensional geometry, that provided for uniform delivery of the reagents, CV values <6% were achieved for the responses of the 10 BB-MZIs, allowing for reliable multianalyte determinations. The analysis is completed in 6.5 min, and the detection limits were 0.04 μg/mL for bovine k-casein, 1.0 μg/mL for peanut protein, 0.80 μg/mL for soy protein, and 0.10 μg/mL for gliadin. The assays were accurate (recoveries 88-118%) and repeatable (intra- and interassay CVs <7% for all four allergens). Finally, the sensor was evaluated by analyzing samples from a cleaning in place system (CIP) of a dairy industry and the results obtained were in good agreement with those received by the respective ELISAs. The analytical characteristics of the sensor combined with the short analysis time and the small chip size make the proposed system an ideal tool for on-site multianalyte determinations.

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Detection of hidden hazelnut protein in food by IgY-based indirect competitive enzyme-immunoassay

Cited by 26 publications

References 10 publications

Rapid Antibody Selection Using Surface Plasmon Resonance for High-Speed and Sensitive Hazelnut Lateral Flow Prototypes

Rapid Antibody Selection Using Surface Plasmon Resonance for High-Speed and Sensitive Hazelnut Lateral Flow Prototypes

Biosensor immunoassay for traces of hazelnut protein in olive oil

Ultrafast Multiplexed-Allergen Detection through Advanced Fluidic Design and Monolithic Interferometric Silicon Chips

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