Detection of high mass‐to‐charge ions by Fourier transform mass spectrometry

Holliman, Christopher L.; Rempel, Don L.; Gross, Michael L.

doi:10.1002/mas.1280130202

Cited by 47 publications

(21 citation statements)

References 71 publications

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“…The earlier results for C 60 at 1.2 T and substance P with quadrupolar excitation at 3.0 T [37,39] anticipate the results reported here. After incorporating the RF-only mode into the 3-T system and replacing quadrupolar excitation, we obtained the spectra shown in Figure 3 of ϩ ions of substance P during the first 63.6 s after the laser desorption event.…”

Section: Resultssupporting

confidence: 87%

“…We found a low resolving power signal with just the RF-only mode operating, a weak, but highly resolved signal with compensation, and finally, an intense high resolving-power signal with both the RFonly-mode event and compensation turned on [36]. We reproduced the trend with C 60 evaporated from a heated solids probe [37]. In a larger 4.76-cm trap in a higher 3.0 T magnetic field, the resolving power not only improved from 25,000 to 1,000,000 via compensation of laser-desorbed C 60 , but could be sustained from shot to shot with a precision of Ϯ 19% [38].…”

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confidence: 58%

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Evaluation of different combinations of gated trapping, RF-only mode and trap compensation for in-field MALDI Fourier transform mass spectrometry

Gooden

Rempel

Gross

2004

J. Am. Soc. Mass Spectrom.

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MALDI, while providing advantages such as the ability to do in-depth and repeated exploration of the sample, challenges the existing performance capabilities of Fourier transform mass spectrometry (FTMS). The challenge arises because MALDI-produced ions have high mass-to-charge ratios and uncertain kinetic-energy distributions. We demonstrate that a combination of a gated trapping event, a RF-only mode pressure focusing event, and an electrically compensated trap provides a compelling advantage in meeting these challenges. Removal of any of the above combination elements significantly degrades the detection performance of substance P from 850 K resolving power at 34.9 kHz and of melittin from 278 K resolving power at 16.5 kHz when using a 3-Tesla magnet-based spectrometer. atrix-assisted laser desorption ionization (MALDI), when interfaced to Fourier transform mass spectrometry (FTMS) in principle has advantages with respect to electrospray ionization (ESI). First, MALDI places a lower vacuum load than does ESI on the instrument, permitting a simplified high vacuum system. Second, the low charge-state ions that are produced by MALDI allow more ions in the trap for a given charge capacity and give better utilization of the limited dynamic range of FTMS. Third, when the sample probe is located at the trap, a simple instrument results, negating the use of an external ion source, transmission ion optics, and associated pumping. The combination of random access and retrieval, a general characteristic of MALDI, and the versatility of FTMS (e.g., accurate mass, ion manipulation and activation) facilitate in-depth and repeated exploration of unknown samples.The shortcomings of FTMS, however, are more apparent with MALDI-produced than ESI-produced ions. The principal problem is that the former ions have higher m/z, lower frequency than do corresponding ESI ions. The former ions often have a lower charge state, causing poor performance because magnetic forces decrease as m/z increases. Use of higher B fields reduces the problem while shifting it to higher m/z. Furthermore, distributions of initially formed MALDI ions are variable and broad with high velocities (up to ϳ 660 m/s) [1], resulting in inefficient "catching" and unreliable and often poor detection.An effective and logical plan to improve MALDI/ FTMS could include three general approaches. The first is to modify the MALDI desorption to attenuate the translational velocities and internal energies of the desorbed ions. Examples of these efforts include the use of a comatrix [2] or a "waiting room," [3] which in the limit becomes high-pressure MALDI [4]. An alternative is the use of an external source [5], which permits the use of quadrupole and higher order traps or ion guides to focus and thermalize the MALDI-produced ions before introducing them into the FTMS trap. The second approach is to introduce efficiently the MALDI ions, whether produced externally or in-field, into the trap. The third is to improve the trap for detection of the high-m/z ions that are oft...

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Section: Resultssupporting

confidence: 87%

supporting

confidence: 58%

Evaluation of different combinations of gated trapping, RF-only mode and trap compensation for in-field MALDI Fourier transform mass spectrometry

Gooden

Rempel

Gross

2004

J. Am. Soc. Mass Spectrom.

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“…Significant advances in FTICR-MS technology have been reported over the last decade (Belov, et al, 2000b, Marshall, et al, 1998, Marshall, 2000, Zubarev, et al, 1998. In addition, there are a number of excellent reviews available on FTICR-MS (Holliman, et al, 1994, Marshall, 1996, Marshall & Guan, 1996, Marshall, et al, 1998, McLafferty, 1994 and its use in proteomics (Bergquist, 2003, Page, et al, 2004. Interested readers are directed to these reviews for additional information.…”

Section: Historical Overview Of Nanolc-esi-fticr-msmentioning

confidence: 99%

Advances in proteomics data analysis and display using an accurate mass and time tag approach

Zimmer

Monroe

Qian

et al. 2006

Mass Spectrometry Reviews

293

360

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Proteomics has recently demonstrated utility in understanding cellular processes on the molecular level as a component of systems biology approaches and for identifying potential biomarkers of various disease states. The large amount of data generated by utilizing high efficiency (e.g., chromatographic) separations coupled to high mass accuracy mass spectrometry for high-throughput proteomics analyses presents challenges related to data processing, analysis, and display. This review focuses on recent advances in nanoLC-FTICR-MS-based proteomics approaches and the accompanying data processing tools that have been developed to display and interpret the large volumes of data being produced.

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“…F ourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) [1,2] is recognized as being a useful analytical instrument because it provides the highest broadband mass spectral resolving power and mass accuracy currently available [3,4]. For example, in order to derive the unique elemental composition from the monoisotopic mass for masses 9500 Da, one needs accuracy at the sub-ppm level or better [5,6], which can currently only be achieved by FT-ICR MS.…”

Section: Introductionmentioning

confidence: 99%

Phase Correction of Fourier Transform Ion Cyclotron Resonance Mass Spectra Using MatLab

Thompson

Orden

et al. 2011

J. Am. Soc. Mass Spectrom.

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FT-ICR mass spectrometry has been limited to magnitude mode for almost 40 years due to the data processing methods used. However, it is well known that phase correction of the data can theoretically produce an absorption-mode spectrum with a mass-resolving power that is as much as twice as high as conventional magnitude mode, and that it also improves the quality of the peak shape. Temporally dispersed frequency sweep excitation followed by a time delay before detection results in a steep quadratic variation in the signal phase with frequency. Viewing this, it is possible to find the correct phase function by performing a quadratic least squares fit, modified by iterating through phase cycles until the correct quadratic function is found. Here, we present a robust manual method to rotate these signals mathematically and generate a "phased" absorption-mode spectrum. The method can, in principle, be automated. Baseline correction is also included to eliminate the accompanying baseline drift. The resulting experimental FT-ICR absorption-mode spectra exhibit a resolving power that is at least 50% higher than that of the magnitude mode.

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Detection of high mass‐to‐charge ions by Fourier transform mass spectrometry

Cited by 47 publications

References 71 publications

Evaluation of different combinations of gated trapping, RF-only mode and trap compensation for in-field MALDI Fourier transform mass spectrometry

Evaluation of different combinations of gated trapping, RF-only mode and trap compensation for in-field MALDI Fourier transform mass spectrometry

Advances in proteomics data analysis and display using an accurate mass and time tag approach

Phase Correction of Fourier Transform Ion Cyclotron Resonance Mass Spectra Using MatLab

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