Detection of human Betaherpesvirinae in saliva and urine from immunocompromised and immunocompetent subjects

Gautheret‐Dejean, Agnès; Aubin, Jean-Thierry; Poirel, Laurent; Huraux, Jean‐Marie; Nicolas, J C; Rozenbaum, Willy; Agut, Henri

doi:10.1128/jcm.35.6.1600-1603.1997

Cited by 44 publications

(20 citation statements)

References 25 publications

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“…These findings were consistent with previous data based on the analysis of an adult population [Yoshikawa et al, 2005]. Additionally, other studies demonstrated that the HHV-7 DNA load in saliva was relatively high in comparison to the other two bherpesviruses [Gautheret-Dejean et al, 1997;Fujiwara et al, 2000]. In addition to the kinetic data, only HHV-7 DNA load in saliva was different among the three different renal diseases (Fig.…”

Section: Discussionsupporting

confidence: 92%

Analysis of the shedding of three β‐herpesviruses in urine and saliva of children with renal disease

Yamamoto

Morooka

Hashimoto

et al. 2013

Journal of Medical Virology

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Cytomegalovirus (CMV), human herpesvirus 6 (HHV-6) and 7 (HHV-7) are important pathogens in immunocompromised patients. To elucidate the kinetics of the three β-herpesviruses in saliva and urine samples were collected serially from children with renal diseases. Twenty children with renal diseases were enrolled in this study. A total of 240 saliva and urine samples were collected monthly from the patients over a 1-year period. Viral DNAs loads were measured by real-time PCR. In 10 CMV seropositive patients CMV DNA was detected rarely in saliva and CMV DNA load was lower than the other two β-herpesviruses DNA loads. All patients were seropositive for HHV-6B and the virus was detected frequently in saliva. Two of 20 patients were HHV-7 seronegative. High copies of viral DNA were detected continuously in saliva of the HHV-7 seropositive patients. Although neither CMV nor HHV-6B DNA load was different among the three renal diseases, HHV-7 DNA load was different among the diseases (P = 0.039). HHV-6B DNA loads were significantly higher in patients with immunosuppressive treatment compared to those without treatment (P = 0.013). Although CMV DNA was detected in urine samples collected from 5 of 10 CMV seropositive patients, HHV-6B and HHV-7 DNA were detected at relatively low frequencies in urine. No remarkable temporal associations between viral DNA excretion and proteinuria or immunosuppressive treatment were demonstrated. The pattern of viral DNA excretion in saliva and urine were different among the three viruses. No temporal correlation was observed between viral infection and renal diseases.

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Section: Discussionsupporting

confidence: 92%

Analysis of the shedding of three β‐herpesviruses in urine and saliva of children with renal disease

Yamamoto

Morooka

Hashimoto

et al. 2013

Journal of Medical Virology

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“…In the latters, the prevalence of HHV-6 was higher at remission and relapse than at diagnosis and aplasia, confirming the reactivation of HHV-6 after chemotherapy [Seror et al, 2008;Faten et al, 2012]. In the saliva samples, the frequencies of HHV-6 DNA detection obtained by other authors for healthy subjects were lower with 3% [Di Luca et al, 1995], higher with 90% and 97% [Cone et al, 1993;Miller et al, 2005] or similar with 62% [Gautheret- Dejean et al, 1997], in comparison with the present study (62%). This may be due to the volume of saliva analyzed, inter-laboratory technical discrepancies or differences of PCR sensitivity.…”

Section: Discussionsupporting

confidence: 82%

“…This may be due to the volume of saliva analyzed, inter-laboratory technical discrepancies or differences of PCR sensitivity. The results observed for HIV-infected patients and multiple sclerosis patients were lower with 38% of detection [Gautheret-Dejean et al, 1997] and similar, with 65% of detection, respectively [Höllsberg et al, 2005]. HHV-6 median load (11,950 copies/ml) was higher than that observed for healthy subjects (5,000 and 1,917 copies/ ml) [Cone et al, 1993;Miller et al, 2005] and was similar to that found in children with renal disease [Yamamoto et al, 2013].…”

Section: Discussionsupporting

confidence: 70%

Quantitative analysis of human herpesvirus‐6 and human cytomegalovirus in blood and saliva from patients with acute leukemia

Nefzi

Abid

Khélif

et al. 2014

Journal of Medical Virology

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Human herpesvirus-6 (HHV-6) and human cytomegalovirus (HCMV) DNAs were quantified by real-time PCR assays in blood and saliva obtained from 50 patients with acute leukemia at the time of diagnosis (50 of each matrix), aplasia (65 of each matrix), remission (55 of each matrix), and relapse (20 of each matrix) to evaluate which biological matrix was more suitable to identify a viral reactivation, search for a possible link between HHV-6 and HCMV reactivations, and evaluate the relations between viral loads and count of different leukocyte types in blood. The median HHV-6 loads were 136; 219; 226, and 75 copies/million cells in blood at diagnosis, aplasia, remission and relapse, respectively. The HCMV loads were 193 and 317 copies/million cells in blood at diagnosis and remission. In the saliva samples, the HHV-6 loads were 22,165; 15,238; 30,214, and 17,454 copies/million cells at diagnosis, aplasia, remission, and relapse, respectively. The HCMV loads were 8,991; 1,461; 2,980, and 4,283 copies/million cells at diagnosis, aplasia, remission, and relapse, respectively. The HHV-6 load in the blood was correlated to the counts of polymorphonuclear leukocytes (R(2) = 0.5; P < 0.0001) and lymphocytes (R(2) = 0.4; P = 0.001) and was not correlated to the monocyte counts (R(2) = 0.07; P = 0.7). Saliva appears to be a more sensitive biological matrix than whole blood in the detection of HHV-6 or HCMV reactivations. The HHV-6 and HCMV reactivations were linked only in saliva.

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“…Human herpesvirus 7 (HHV-7) was originally isolated from the stimulated CD4 ϩ T cells of a healthy individual (9) and was subsequently characterized as a ubiquitous virus, infecting most human beings (5,10,17,25,27). This virus was classified in the Betaherpesvirinae subfamily on the basis of its genetic organization (3,6,15,16).…”

mentioning

confidence: 99%

Preferential Associations of Alleles of Three Distinct Genes Argue for the Existence of Two Prototype Variants of Human Herpesvirus 7

Franti

Aubin

Gautheret‐Dejean

et al. 1999

J Virol

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We had previously described six distinct alleles of the glycoprotein B (gB) gene of human herpesvirus 7 (HHV-7). The genetic changes corresponding to these alleles did not affect gB gene transcription or translation in in vitro assays. The study of distinct HHV-7-positive human samples showed preferential associations of some gB alleles with some alleles of two other genes, distantly located on the HHV-7 genome, coding for the phosphoprotein p100 (p100) and the major capsid protein (MCP). Two allele combinations, corresponding to 44 and 31% of the samples studied, respectively, were interpreted as the genetic signatures of two major prototype HHV-7 variants.

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Detection of human Betaherpesvirinae in saliva and urine from immunocompromised and immunocompetent subjects

Cited by 44 publications

References 25 publications

Analysis of the shedding of three β‐herpesviruses in urine and saliva of children with renal disease

Analysis of the shedding of three β‐herpesviruses in urine and saliva of children with renal disease

Quantitative analysis of human herpesvirus‐6 and human cytomegalovirus in blood and saliva from patients with acute leukemia

Preferential Associations of Alleles of Three Distinct Genes Argue for the Existence of Two Prototype Variants of Human Herpesvirus 7

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