Detection of human cytomegalovirus genome and gene products in central nervous system tumours

Sabatier, Jean; Uro‐Coste, Emmanuelle; Pommepuy, Isabelle; Labrousse, François; Allart, Sophie; Trémoulet, M; Mj, Delisle; Brousset, Pierre

doi:10.1038/sj.bjc.6602339

Cited by 75 publications

(64 citation statements)

References 14 publications

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“…Indeed, one group only has reported on the detection of this virus in CNS tumours and also in colonic and prostate cancers (Samanta et al, 2003). As far as the CNS tumours are concerned, we have been unable to reproduce the results of the study by Cobbs et al We have found that the virus could be detected in only a few cases of CNS tumours not in malignant cells but rather in putative stromal cells (Sabatier et al, 2005; data not shown).…”

Section: Discussioncontrasting

confidence: 51%

Detection of oncogenic virus genomes and gene products in lung carcinoma

et al. 2005

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We investigated a series of 122 cases of small cell lung carcinomas and non-small cell lung carcinomas for the presence of several viruses that are known to be oncogenic in humans. Thus, viral genomes (DNA) and/or RNA transcripts and/or proteins of human papillomaviruses (HPV) 16, 18, 31, 33, 51, Epstein -Barr virus (EBV), human herpesvirus 8 (HHV-8), human cytomegalovirus (HCMV) and simian virus 40 (SV40) were investigated on tissue sections (prepared in tissue microarrays) with different techniques of immunohistochemistry and in situ hybridisation. None of the cases displayed a single positive tumour cell for all the viruses tested whatever the technique applied. Of note, in five cases of tumours with lymphoid infiltrates, we detected scattered EBV (EBER)-positive bystander lymphocytes. In three cases, a faint nuclear staining was found with the anti-latent nuclear antigen/LANA1 (HHV-8) antibody. These cases were checked by PCR with two sets of primers (orf 26 and orf 75) and remained negative for this latter virus. Taken together, our data strongly suggest that the conventional human oncogenic viruses (HPV, EBV, HCMV, HHV-8 and SV40) are unlikely to play some role in the development of lung carcinomas.

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Section: Discussioncontrasting

confidence: 51%

Detection of oncogenic virus genomes and gene products in lung carcinoma

et al. 2005

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“…Furthermore, formalin-fixed, paraffin-embedded samples were obtained from two adult patients with HCMV reactivation. Cell lysates from fresh frozen tissues from patients #1, 3,4,8,18 in Table 1 were applied for immunoblotting. Paraffin sections from patients #41-50 and #P3 in Table 1 were applied for immunohistochemistry and FISH.…”

Section: Patientsmentioning

confidence: 99%

Lack of presence of the human cytomegalovirus in human glioblastoma

et al. 2014

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Recent reports have indicated human cytomegalovirus (HCMV) to be associated with human glioblastoma carcinogenesis. In established examples of viral carcinogenesis, viral DNA and one or more of its products have been detected in most tumor cells of biopsies in the majority of cases. To test whether HCMV is associated with human glioblastoma based on this criterion, we measured the number of viral DNA molecules per cell in both frozen and paraffin-embedded tumor biopsies from 58 patients using real-time quantitative PCR (QPCR). Immunohistochemical and fluorescence in situ hybridization (FISH) to detect HCMV proteins and genome was performed in 10 cases using formalin-fixed paraffin-embedded glioblastoma tissues. Southern blotting using DNA extracted from four glioblastoma cell lines together with immunoblotting using the four cell lines and five glioblastoma tissue samples were also performed. We further confirmed the immunoblot bands using liquid chromatography-tandem mass spectrometry assay. As a result, HCMV DNA was not detected in the tumor cells from any of the glioblastoma cases by QPCR detecting two different HCMV genes, in clear contrast to samples from patients with HCMV infection. Southern blotting and immunoblotting of cell lines and FISH using paraffin sections were all negative. However, immunoblotting and immunohistochemistry using tissue samples were partly positive, but HCMV proteins were not detected by proteomic analysis, suggesting false positivity of the analyses. As our QPCR analysis could detect 10 copies of HCMV DNA mixed with DNA extracted from 10 4 HCMV-negative cells, we conclude that HCMV is not persistent, at least in the tumor cells, of developed human glioblastoma.

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“…In contrast, Sabatier et al (2005) screened 132 CNS tumours, including 97 GBMs, by IMH (anti-IE1 monoclonal antibody) and ISH with tissue microarrays and frozen sections and found rare astrocyte-like cells staining positive for HCMV in only 10 cases of GBM and questionable staining of rare cells in one case. This result suggested that none of the CNS tumours contained HCMV genomes and/or proteins in a significant proportion of tumour cells.…”

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confidence: 99%