Detection of human papillomavirus DNA in oral inverted ductal papillomas

Abstract

Aims: To determine the presence or absence of human papillomavirus (HPV) DNA in oral inverted ductal papillomas (IDPs) using in situ hybridisation (ISH), and to analyse all cases for histological features of HPV infection. Methods: Six cases were retrieved from archival material and paraffin wax blocks were submitted for the detection of HPV DNA by means of ISH. A wide spectrum probe for HPV subtypes 6,11,16,18, 31, 33, 45, 51, and 52 was used initially. Cases that were positive using this wide spectrum probe were further subtyped using HPV type specific probes (6/11, 16/18, and 31/33). The histological features of all tumours were analysed using routine microscopy. Results: Of the six cases of oral IDP identified, three were positive for HPV subtypes 6/11. All positive cases showed histological features of HPV infection (koilocytosis, papillomatosis, binucleated keratinocytes, and abnormal mitosis) in both the surface and the inverted epithelium. The three cases that tested negative for HPV DNA also exhibited focal histological features of HPV infection (two in the surface epithelium and one in the endophytic epithelium). Conclusions: These are the first documented cases of oral IDP to demonstrate positivity for HPV DNA and also to show histological features of HPV infection.

Oral inverted ductal papilloma associated with condyloma acuminata and HPV in an HIV+ patient

“…Two previous studies were unable to confirm the presence of HPV DNA using ISH and PCR 6,8 . HABERLAND-CARRODEGUAS et al 5 published the first report documenting the presence of HPV DNA in oral IDP, detecting HPV 6/11 DNA in half of the cases studied. The present report supports the existence of HPV 11 in oral IDP and CA of a HIV+ patient.…”

“…Since human papilloma virus (HPV) has been identified in sinonasal papilloma by polymerase chain reaction (PCR) and in situ hybridisation (ISH) techniques, many authors have searched for the presence of HPV in oral IDP because of the histological similarity between oral IDP and inverted papilloma of the nose and paranasal sinuses. In 2003, HABERLAND-CARRODEGUAS et al 5 documented the first cases of oral IDP demonstrating positivity for HPV DNA 6/11 using ISH in three cases and showing histological features of HPV infection in six cases. The authors report, for the first time, the simultaneous appearance of an IDP and two condyloma acuminata (CA) associated with HPV 11 in the lips of a middle-aged HIV+ woman, and compare the histopathologic findings of the samples using routine light microscope and PCR for HPV DNA.…”

“…In 2005, the World Health Organization's (WHO) histological classification of salivary gland tumours, classified IDP as a ductal papilloma, along with intraductal papilloma and sialadenoma papilliferum 2 . The incidence of IDP is unknown, almost 50 cases of IDPs have been published in the English-language literature [3][4][5] . Clinically, all lesions present as a soft submucosal nodule of either smooth or papillary surface, with or without a central punctum.…”

Koilocytes indicate a role for human papilloma virus in breast cancer

“…Putative koilocytes have been identified in HPV 6/11-associated benign laryngeal papillomas (Martins et al, 2008), high-risk HPV 16-associated malignant oesophageal lesions (Miller et al, 1997), high-risk HPV 11-associated conjunctival papilloma (Minchiotti et al, 2006), HPV 6/11-associated benign ductal papillomas of the salivary glands (Haberland-Carrodeguas et al, 2003), and high-risk HPV 16 in bladder cancers (Aggarwal et al, 2009). These associations between the presence of HPVs and koilocytosis are in addition to their well-established associations in anogenital cancers (Boon and Kok, 1985).…”

Análise morfológica microscópica de Mucoceles na mucosa bucal e inter-relação com sua etiopatogenia

“…Haberland-Carrodeguas et al (2003) demonstraram pela primeira vez a presença de infecção por HPV no Papiloma Ductal Invertido em 3 de 6 casos relatados.…”