Detection of Human Papillomavirus in Laryngeal Squamous Dysplasia and Carcinoma. An<i>In Situ</i>Hybridization and Signal Amplification Study

Brito, Hugo Leite de Farias

doi:10.1080/000164800750046072

Cited by 17 publications

(13 citation statements)

References 19 publications

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“…For example, HPV detected in four out of 45 laryngeal tumors by PCR was limited to the superficial and intermediate epithelial layers when examined by in situ hybridization and was not present in the invasive neoplastic cords [Brito et al, 2000]. This may suggest that secondary superinfection was not related pathologically to the tumor.…”

Section: Discussionmentioning

confidence: 97%

Association between human papillomavirus infection and laryngeal squamous cell carcinoma

Morshed

2010

Journal of Medical Virology

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The aim of this study was to compare the prevalence of human papillomavirus (HPV) infection in laryngeal squamous cell carcinoma using two methods: PCR-DNA enzyme immunoassay (PCR/DEIA) and immunohistochemistry (IHC) for detection of HPV in specimens of laryngeal squamous cell carcinoma and to correlate the presence of HPV with the epidemiological and clinicopathological features of recurrence and survival. HPV DNA was amplified from 93 paraffin-embedded laryngeal squamous cell carcinoma tissue specimens by the short PCR fragment (SPF 10) primer set using PCR/DNA method. HPV detection using monoclonal anti-human papilloma virus antibodies Clone K1H8 for IHC reaction was performed on 130 specimens. HPV was identified in 35.5% of patients with laryngeal squamous cell carcinoma using PCR/DEIA and 27.7% using IHC. There was no statistically significant association between the presence of HPV and the epidemiological and clinicopathological features and recurrence. There was no statistically significant association between the presence of HPV and overall survival nor disease specific survival. Statistically significant correlation between HPV detection using PCR/DEIA technique and IHC technique was found. The presence of HPV infection in 27.7% and 38.9% of the patients suggests a possible role in the etiology of laryngeal squamous cell carcinoma. The SPF(10) PCR/DEIA technique is the most accurate method for detection of HPV in laryngeal squamous cell carcinoma.

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Section: Discussionmentioning

confidence: 97%

Association between human papillomavirus infection and laryngeal squamous cell carcinoma

Morshed

2010

Journal of Medical Virology

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“…The frequency of HPV-infections in these lesions is in accordance with previous studies. [38][39][40][41] Remarkably, all 20 samples of tonsillar papillomas were HPV-negative.…”

Section: Hpv-status Of the Head And Neck Lesions Examined In This Studymentioning

confidence: 96%

P16^INK4A immunostaining is a strong indicator for high‐risk‐HPV‐associated oropharyngeal carcinomas and dysplasias, but is unreliable to predict low‐risk‐HPV‐infection in head and neck papillomas and laryngeal dysplasias

Mooren

Gültekin

Straetmans

et al. 2013

Intl Journal of Cancer

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Human papillomavirus (HPV) is a risk factor for the development of benign and malignant mucosal head and neck lesions. P16 INK4A is often used as a surrogate marker for HPV-infection, although there is still controversy with respect its reliability. Our aim was to determine if p16 INK4A overexpression can accurately predict both high-risk and low-risk-HPV-presence in (pre)-malignant and benign head and neck lesions. P16 INK4A immunohistochemistry was performed on paraffin-embedded tissue sections of 162 oropharyngeal squamous cell carcinomas (OPSCC), 14 tonsillar and 23 laryngeal dysplasias, and 20 tonsillar and 27 laryngeal papillomas. PCR, enzyme-immunoassay and FISH analysis were used to assess HPV-presence and type. Of the 162 OPSCC and 14 tonsillar dysplasias, 51 (31%) and 10 (71%) were HPV16-positive, respectively. All tonsillar papillomas were HPV-negative and four laryngeal dysplasias and 26 laryngeal papillomas were positive for HPV6 or 211. P16 INK4A immunohistochemistry revealed a strong nuclear and cytoplasmic staining in 50 out of 51 HPV16-positive and 5 out of 111 HPVnegative OPSCC (p < 0.0001) and in all HPV16-positive tonsillar dysplasias, whereas highly variable staining patterns were detected in the papillomas and laryngeal dysplasias, irrespective of the HPV-status. In addition, the latter lesions generally showed a higher nuclear than cytoplasmic p16 INK4A immunostaining intensity. In conclusion, our data show that strong nuclear and cytoplasmic p16 INK4A overexpression is a reliable surrogate indicator for HPV16 in OPSCC and (adjacent) dysplasias. For HPV6 or 211-positive and HPV-negative benign and premalignant lesions of the tonsil and larynx, however, p16 INK4A immunostaining is highly variable and cannot be recommended to predict HPV-presence.

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“…In the same fashion as laryngeal cancer, the prevalence of HPV infection in laryngeal SILs has varied widely, between 0% and 56%, with an overall prevalence of HPV infection of 12.4% 94–101 . HPV+ SILs harbour mainly high‐risk HPV genotypes, with HPV‐16 being the most frequent.…”

Section: Aetiologymentioning

confidence: 99%

Current review on squamous intraepithelial lesions of the larynx

Gale¹,

Michaels²,

Luzar³

et al. 2009

Histopathology

122

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Squamous intraepithelial lesions (SILs) of the larynx, clinically usually defined as leukoplakia and chronic laryngitis, have remained the main controversial topic in laryngeal pathology for decades as regards classification, histological diagnosis and treatment. SILs are caused by smoking and alcohol abuse. There is also mounting evidence that gastroesophageal reflux is a potential aetiological factor. Human papillomavirus infection seems to play little if any role in laryngeal carcinogenesis. Histological classification of SILs is the central disputed aspect of these lesions. There are as yet no generally accepted criteria for histological grading of laryngeal SILs. Three currently used classifications of SILs are reviewed here: the dysplasia system, the Ljubljana classification and the binary system of squamous intraepithelial neoplasia. One of the most important issues of SILs is the risk of malignant transformation. Data in the literature are controversial because of inconsistent use of morphological criteria in different classifications. It is often difficult for clinicians to agree on the most appropriate therapeutic option for a particular grade of SIL that has been diagnosed. Transition from normal epithelium to SILs and squamous cell carcinoma is related to progressive accumulation of genetic changes leading to a clonal population of transformed epithelial cells. Despite extensive research into these genetic changes in laryngeal carcinogenesis, reliable genetic markers with diagnostic and prognostic value are still lacking.

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Detection of Human Papillomavirus in Laryngeal Squamous Dysplasia and Carcinoma. AnIn SituHybridization and Signal Amplification Study

Cited by 17 publications

References 19 publications

Association between human papillomavirus infection and laryngeal squamous cell carcinoma

Association between human papillomavirus infection and laryngeal squamous cell carcinoma

P16^INK4A immunostaining is a strong indicator for high‐risk‐HPV‐associated oropharyngeal carcinomas and dysplasias, but is unreliable to predict low‐risk‐HPV‐infection in head and neck papillomas and laryngeal dysplasias

Current review on squamous intraepithelial lesions of the larynx

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Detection of Human Papillomavirus in Laryngeal Squamous Dysplasia and Carcinoma. AnIn SituHybridization and Signal Amplification Study

Cited by 17 publications

References 19 publications

Association between human papillomavirus infection and laryngeal squamous cell carcinoma

Association between human papillomavirus infection and laryngeal squamous cell carcinoma

P16INK4A immunostaining is a strong indicator for high‐risk‐HPV‐associated oropharyngeal carcinomas and dysplasias, but is unreliable to predict low‐risk‐HPV‐infection in head and neck papillomas and laryngeal dysplasias

Current review on squamous intraepithelial lesions of the larynx

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P16^INK4A immunostaining is a strong indicator for high‐risk‐HPV‐associated oropharyngeal carcinomas and dysplasias, but is unreliable to predict low‐risk‐HPV‐infection in head and neck papillomas and laryngeal dysplasias