Detection of human papillomavirus infection by molecular tests and its relation to colonic polyps and colorectal cancer

Abstract

Objectives:To prospectively examine the association between human papilloma virus (HPV) colonization of the colonic mucosa and the development of colorectal polyps (CRPs), and colorectal cancer (CRC) in Saudi Arabia.Methods:A case control study was performed between January 2013 and December 2014. All eligible patients underwent standard diagnostic colonoscopy. Patients with polyps or colorectal cancer were considered cases, while those with any other endoscopic findings were controls. Biopsy samples from polyps and tumors, and/or from normal colonic mucosa were acquired. Human papilloma virus colonization was detected using a hybrid capture technique of samples taken from both normal tissue, and CRPs and CRC. The association between HPV and CRPs/CRC was evaluated.Results:A total of 132 patients were recruited. The mean age was 53 (±15.9) years. Sixty patients had endoscopically detectable CRPs/CRC, and 72 had either inflammation or normal endoscopic evaluations. Only 4 (0.8%) of the 132 samples that were collected and analyzed were positive for the HPV gene. Statistical analysis did not identify any significant association between HPV colonization and the presence of CRPs/CRC. The only significant predictor of detecting CRPs/CRC on colonoscopy was symptomatic presentation (odds ratio=11.072, 95% confidence interval 4.7-26.2, p<0.001).Conclusion:Human papilloma virus colonic colonization is rare in Saudi Arabia. An association between HPV colonization and CRP/CRC development could not be identified in this cohort of patients.

HPV and the Development of Colorectal Cancer

“…Our outcomes are consistent with other studies, which could not reveal the DNA of HPV in colorectal tumors and controls (Gazzaz et al, 2016;Aghakhani et al, 2014;Taherian et al, 2014;Yavuzer et al, 2011;Gornick et al, 2010;Urisman et al, 2006;Perez et al, 2005;Boguszakova et al, 1988;Shah et al, 1992). In one of these studies conducted by Aghakhani et al (2014), the team examined the incidence of HPV genome in 70 samples of colorectal neoplasms, and 30 patients with adenoma and 30 tumor adjacent tissues (as control).…”

“…Later in 1995, Cheng et al (1995) were the first to report HPV involvement in colorectal carcinoma. However, HPV has continued to be a debatable subject as a carcinogenic agent of colorectal cancer (Gazzaz et al, 2016;Aghakhani et al, 2014;Taherian et al, 2014;Burnett-Hartman et al, 2011;Gornick et al, 2010;Damin et al, 2007;Perez, Abba, Laguens, & Golijow, 2005;Shah, Daniel, Simons, & Vogelstein, 1992;Shroyer et al, 1992). Furthermore, the exact role of HPV in colorectal pathogenesis is somehow not clear.…”

“…A relationship between the development of malignant colorectal tumors and human papilloma virus infection was described (Yavuzer et al, 2011;Burnett-Hartman et al, 2008;Damin et al, 2007). However, HPV aetiology of colorectal cancer is still an intriguing subject (Gazzaz, Mosli, Jawa, & Sibiany 2016;Aghakhani et al, 2014;Taherian, Tafvizi, Fard & Abdirad, 2014;Gornick et al, 2010).…”

Detection of Human papillomavirus and the role of p16INK4a in colorectal carcinomas

“…Genotyping was performed by reverse hybridization using the INNO-LiPA HPV Genotyping Extra II Kit (Fujirebio Europe 1 ). The assay allows the identification of 32 HPV genotypes, currently known as high risk genotypes [16,18,31,33,35,39,45,51,52,56,58,59,68], probable high risk [26,53,66,70,73,82] and low risk [6,11,40,42,43,44,54,61,62,67,81,83,89].…”

Büyük adenomatöz kolorektal poliplerde insan papilloma virüs varlığının araştırılması

“…Mehmet Ali ERDOĞAN 146 mainly to cervix cancer as well as other mucosal tumors. 3 Given the fact that HPV is associated to anogenital or oropharyngeal cancers, studies have been conducted to investigate relationship between HPV and CRC; however, such a relationship is unclear. Besides studies showing relationship between HPV and CRCs, there are also studies proposing vice versa.…”