Detection of human papillomavirus infection in squamous tumours of the conjunctiva and lacrimal sac by immunohistochemistry, in situ hybridisation, and polymerase chain reaction

Nakamura, Yu; Mashima, Yukihiko; Kameyama, Kaori; Mukai, Makio; Oguchi, Yoshihisa

doi:10.1136/bjo.81.4.308

Cited by 105 publications

(56 citation statements)

References 32 publications

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“…Comparing methods, PCR analysis showed to be similar to immunohistochemistry and superior to the in situ hybridization for detecting HPV in squamous tumors of the conjunctiva and lacrimal sac (13) . Using the same method as our, HPV DNA by nested PCR was also negative in all samples studied by others in pterygia, pingueculae and limbal tumors (4) .…”

Section: Discussionmentioning

confidence: 99%

Lack of papillomavirus (HPV) in pterygia of a Brazilian sample

Schellini¹,

Hoyama²,

Shiratori³

et al. 2006

Arq. Bras. Oftalmol.

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Section: Discussionmentioning

confidence: 99%

Lack of papillomavirus (HPV) in pterygia of a Brazilian sample

Schellini¹,

Hoyama²,

Shiratori³

et al. 2006

Arq. Bras. Oftalmol.

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“…[8][9][10][11][12] However, benign conjunctival lesions have been shown to contain the infection as well. [13][14][15][16] Tuppurainen et al 17 failed to demonstrate HPV DNA in conjunctival malignancy by using in situ hybridisation and polymerase chain reaction (PCR).…”

Section: -12mentioning

confidence: 99%

“…There have been a number of investigations on the relation between the tumour to several factors, including solar exposure [5][6][7] and human papillomavirus (HPV). [8][9][10][11][12] Solar exposure has been observed to cause the epithelial malignancy. Newton et al 5 related the prevalence of this malignancy to tropical location of the patient dwellings.…”

mentioning

confidence: 99%

Risk factors for conjunctival squamous cell neoplasia: a matched case-control study

Tulvatana

Bhattarakosol²,

Sansopha³

et al. 2003

British Journal of Ophthalmology

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Aims: To identify roles of human papillomavirus (HPV) infection and solar elastosis as the risk factors for conjunctival squamous cell neoplasia (CSCN). Methods: 30 consecutive pathological specimens, ranging from conjunctival intraepithelial neoplasia, carcinoma in situ, to invasive squamous cell carcinoma were retrieved from tissue archives. 30 controls were disease free conjunctiva from age and sex matched patients undergoing extracapsular cataract extraction. Two masked pathologists studied haematoxylin and eosin stains on paraffin embedded conjunctival tissues. Elastic stain for solar elastosis was blindly interpreted in comparison with negative and positive controls. HPV infection was studied by polymerase chain reaction and dot hybridisation. Results: The mean age of CSCN patients was 54.9 years. The male to female ratio was 1:1. Solar elastosis was seen in 53.3% of CSCN and in 3.3% of controls with an odds ratio of 16.0 (95% CI, 2.49 to 670.96; p value = 0.0003). HPV DNA were not detected in any of the specimens. Conclusion: Solar elastosis is much more frequently found in CSCN cases than in their matched controls and is a risk factor for CSCN. These data are insufficient to conclude that HPV infection is a risk factor for CSCN. C onjunctival squamous cell neoplasia (CSCN) is the most common malignant tumour of the ocular surface.1 2 The disease is prevalent in tropical areas including Thailand.3 4 Early manifestations are small masses at or around the limbus mimicking pterygia, occurring in middle aged patients. The tumours then grow slowly, invading the nearby tissues including the eyeball, eyelids, and orbital tissues leading to severe visual loss, loss of the eye, and severe facial deformities.Similar to the squamous cell carcinoma of the uterine cervix, the stagings of conjunctival squamous cell carcinoma are classified by the thickness of epithelial dysplastic changes and the tumour invasion into the substantia propria. The disease severity varies from conjunctival intraepithelial neoplasia (CIN), carcinoma in situ (CIS), to invasive squamous cell carcinoma.So far the causes of the disease are not adequately understood. There have been a number of investigations on the relation between the tumour to several factors, including solar exposure 5-7 and human papillomavirus (HPV). 8-12Solar exposure has been observed to cause the epithelial malignancy. Newton et al 5 related the prevalence of this malignancy to tropical location of the patient dwellings. Sun et al 6 found an association between ultraviolet exposure and the prevalence of squamous cell neoplasia of the conjunctiva and the eyelids. However, these two studies were conducted as non-comparative studies. Lee et al 7 found a relation of sun exposure to squamous cell dysplasia in a case-control study, but did not include the pathological study on the actinic damage of the tissue.A number of studies have been successful in detecting HPV type 16 and 18 in CSCN. [8][9][10][11][12] However, benign conjunctival lesions have been shown to contain the ...

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“…Furthermore, current findings strongly support a potential active role of HPVs in the development of malignant melanoma [12][13]. As far as ocular tumours are concerned, they have also been aetiologically linked to PV infection both in humans and in cattle [14][15][16][17]. Recently, Van Doorslaer et al [18] have determined the complete nucleotide sequence of Capra hircus PV-type 1 (ChPV-1), after amplification from healthy goat skin.…”

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confidence: 99%